A5007164434- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Biochemical and Molecular Research
- Synthetic Organic Chemistry Methods
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- Chemical Synthesis and Analysis
- Marine Sponges and Natural Products
- Phytochemical compounds biological activities
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- Steroid Chemistry and Biochemistry
- Insect symbiosis and bacterial influences
- Glycosylation and Glycoproteins Research
- Chemical synthesis and alkaloids
- Alkaloids: synthesis and pharmacology
- Antimicrobial Peptides and Activities
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- Plant Gene Expression Analysis
- Phytochemistry and Bioactive Compounds
- Molecular spectroscopy and chirality
- Click Chemistry and Applications
- Viral Infectious Diseases and Gene Expression in Insects
Kangwon National University
2022-2024
Seoul National University
2020-2022
New Generation University College
2022
Ewha Womans University
2010-2020
Seoul Institute
2019
Government of the Republic of Korea
2014-2019
Ewha Womans University Medical Center
2011-2019
Sun Moon University
2012
Scripps Institution of Oceanography
2012
Dongduk Women's University
2012
The allyl moiety of the immunosuppressive agent FK506 is structurally unique among polyketides and critical for its potent biological activity. Here, we detail biosynthetic pathway to allylmalonyl-coenzyme A (CoA), from which group derived, based on a comprehensive chemical, biochemical, genetic interrogation three gene clusters. discrete polyketide synthase (PKS) with noncanonical domain architecture presumably in coordination fatty acid host catalyzes multistep enzymatic reaction...
Heterologous expression of the barbamide biosynthetic gene cluster, obtained from marine cyanobacterium Moorea producens, in terrestrial actinobacterium Streptomyces venezuelae, resulted production a new congener 4-O-demethylbarbamide, demonstrating potential this approach for investigating assembly and tailoring complex natural products.
Doxorubicin, one of the most widely used anticancer drugs, is composed a tetracyclic polyketide aglycone and l-daunosamine as deoxysugar moiety, which acts an important determinant its biological activity. This exemplified by fewer side effects semisynthetic epirubicin (4'-epi-doxorubicin). An efficient combinatorial biosynthetic system that can convert exogenous ε-rhodomycinone into diverse glycosylated derivatives doxorubicin or intermediates, rhodomycin D daunorubicin, was developed...
FK506 is an important 23-member polyketide macrolide with immunosuppressant activity. Its entire biosynthetic gene cluster was previously cloned from Streptomyces sp. strain KCTC 11604BP, and sequence analysis identified three putative regulatory genes, tcs2, tcs7, fkbN, which encode proteins high similarity to the AsnC family transcriptional regulators, LysR-type LAL respectively. Overexpression in-frame deletion of tcs2 did not affect production or co-occurring FK520 compared results for...
Chemical studies of gut bacteria the carpenter ant Camponotus kiusiuensis led to discovery two new alkaloids, camporidines A and B (1 2), from Streptomyces sp. STA1. The structures 1 2 were established as polyketide alkaloids bearing a piperidine–cyclopentene–epoxide 6/5/3 tricyclic system based on NMR spectroscopic mass spectrometric analysis. relative configurations determined by their 1H–1H NOESY/ROESY 1D NOE correlations. experimental ECD spectra compared with calculated assign absolute...
O-Methylated phenylpropanoids, which are generally present in small amounts plants, have improved or distinct biological activities and pharmacological properties as opposed to their unmethylated counterparts. Although microbial production could be a useful tool for the efficient environment-friendly of methylated high-yield neither tri-methylated stilbenes nor di-/tri-methylated flavonoids has been achieved date.A methyltransferase from Streptomyces avermitilis (SaOMT2), known possess...
Systematic inactivation of nonribosomal peptide synthetase (NRPS) domains and translocation the thioesterase (TE) domain revealed several unprecedented nonlinear NRPS assembly processes during biosynthesis cyclodepsipeptide WS9326A in Streptomyces sp. SNM55. First, two sets type ΙΙ TE (TEΙΙ)-like enzymes mediate shuttling activated amino acids between stand-alone adenylation (A)-thiolation (T) didomain modules an "A-less" condensation (C)-T module with distinctive specificities...
The polyketide synthase (PKS) biosynthetic code has recently expanded to include a newly recognized group of extender unit substrates derived from α,β-unsaturated acyl-CoA molecules that deliver diverse side chain chemistry backbones. Herein we report the identification three-gene operon responsible for biosynthesis PKS building block isobutyrylmalonyl-CoA associated with macrolide ansalactam A marine bacterium Streptomyces sp. CNH189. Using synthetic biology approach, engineered production...
A genomic and spectroscopic signature-based search revealed a cycloaromatized enediyne, jejucarboside (1), from marine actinomycete strain. The structure of 1 was determined as new cyclopenta[a]indene glycoside bearing carbonate functionality by nuclear magnetic resonance, high-resolution mass spectrometry (MS), MS/MS, infrared spectroscopy, modified Mosher's method. An iterative enediyne synthase pathway has been proposed for the putative biosynthesis analysis. Jejucarboside exhibited...
A new polyol polyketide, named retinestatin (1), was obtained and characterized from the culture of a Streptomyces strain, which isolated subterranean nest termite Reticulitermes speratus kyushuensis Morimoto. The planar structure 1 elucidated on basis cumulative analysis ultraviolet, infrared, mass spectrometry, nuclear magnetic resonance spectroscopic data. absolute configuration at 12 chiral centers successfully assigned by employing J-based in combination with ROESY correlations, quantum...
The post-PKS modification steps of FK506 biosynthesis include C9-oxidation and 31-O-methylation, but the sequence these reactions exact route have remained unclear. This study details pathways in through identification all intermediates vitro enzymatic cytochrome P450 hydroxylase FkbD methyltransferase FkbM. These results complete our understanding to showing substrate flexibility two enzymes involved existence parallel biosynthetic routes FK506.
Aims: This research aims to investigate the efficiency of two lipolytic enzymes – fungal cutinase and yeast esterase upon biodegradation dihexyl phthalate (DHP). Method Results: During enzymatic degradation DHP dissolved in methanol, several products were detected their time‐course changes monitored using GC/MS. The DHP‐degradation rate was surprisingly high; i.e. almost 70% initial (500 mg l−1) decomposed within 4·5 h. Although same amount employed, more than 85% remained after 3 days....
The chemical analysis of a Streptomyces strain, from Korean volcanic island, discovered new benz[a]anthracene dimers linked by thioether bond. structures donghaesulfins A and B (1 2) were elucidated spectroscopic including energy-dispersive X-ray. Their configurations determined ROESY NMR data, DP4 calculations, the modified Mosher's method, ECD calculations. Donghaesulfins (1) induced quinone reductase, whereas donghaesulfin (2) displayed antiangiogenesis activity.
Two new nonribosomal peptides, bonnevillamides D and E (1 2), have been discovered in Streptomyces sp. UTZ13 isolated from the carrion beetle, Nicrophorus concolor. Combinational analysis of UV, MS, NMR spectroscopic data revealed that their planar structures were comprised dichlorinated linear peptides containing nonproteinogenic amino acid residues, such as 4-methylazetidinecarboxylic 4-O-acetyl-5-methylproline. The configurations 2) determined based on ROESY correlations, advanced...