A5029616013- Microbial Natural Products and Biosynthesis
- Biochemical and Molecular Research
- Signaling Pathways in Disease
- Laser Applications in Dentistry and Medicine
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Pharmacological Effects of Natural Compounds
- Enzyme Production and Characterization
- Phytochemical compounds biological activities
- Cancer therapeutics and mechanisms
- Bee Products Chemical Analysis
- Skin Protection and Aging
- Hormonal and reproductive studies
- Photodynamic Therapy Research Studies
- Gut microbiota and health
- Protein Kinase Regulation and GTPase Signaling
- Chemical Synthesis and Analysis
- Probiotics and Fermented Foods
- Microbial Applications in Construction Materials
- Biopolymer Synthesis and Applications
- Infrared Thermography in Medicine
- Ginseng Biological Effects and Applications
- Neurogenetic and Muscular Disorders Research
Dankook University
2013-2024
Ewha Womans University
2008-2012
Myongji University
2012
Portland State University
2008-2011
Virginia Commonwealth University
2005
The allyl moiety of the immunosuppressive agent FK506 is structurally unique among polyketides and critical for its potent biological activity. Here, we detail biosynthetic pathway to allylmalonyl-coenzyme A (CoA), from which group derived, based on a comprehensive chemical, biochemical, genetic interrogation three gene clusters. discrete polyketide synthase (PKS) with noncanonical domain architecture presumably in coordination fatty acid host catalyzes multistep enzymatic reaction...
FK506 is an important 23-member polyketide macrolide with immunosuppressant activity. Its entire biosynthetic gene cluster was previously cloned from Streptomyces sp. strain KCTC 11604BP, and sequence analysis identified three putative regulatory genes, tcs2, tcs7, fkbN, which encode proteins high similarity to the AsnC family transcriptional regulators, LysR-type LAL respectively. Overexpression in-frame deletion of tcs2 did not affect production or co-occurring FK520 compared results for...
Soybean pods, separated and enclosed from the outside environment, are considered a suitable place to find new microbes. A Gram-stain-negative, aerobic bacterium, bacterial strain (YB22T) was isolated pod of Glycine max (soybean) collected rural area in Republic Korea characterized by using polyphasic taxonomy. Cells were rod-shaped (approximately 0.4-0.6 µm wide 4.0-5.0 long), non-flagellated formed silver-yellow colonies. grew at 25-35 °C (optimum, 28-30 °C), pH 5.0-9.0 7.0) with 0-2.0%...
Clostridium butyricum is a butyric acid-producing anaerobic bacteriuma, and commonly present as gut microbiota in humans. This species has been used probiotic for the prevention of diarrhea In this study, we report draft genome C. DKU-01, which was isolated from infant feces, to better understand characteristics strain so that it can later be development products.A total 79 contigs generated by hybrid assembly sequences obtained Roche 454 Illumina Miseq sequencing systems were investigated....
A novel extracellular phospholipase C (PLC) was purified from a marine streptomycete. It had molecular mass of 28 kDa as estimated by SDS-polyacrylamide gel electrophoresis. Its enzyme activity optimal at pH 8.0 45 °C. The PLC hydrolyzed only phosphatidylcholine. enhanced 300% Na+ (200 mm), suggesting that the is typical marine-type enzyme.
Abstract We evaluated changes in cell viability and morphology response to low‐level light irradiation underlying variations the levels of heat shock proteins (HSPs). Human fibroblasts were irradiated with a light‐emitting diode (LED) array at 660 nm (50 mW for 15, 30, 60 minutes). Cell morphological via epifluorescence analysis; we also assessed length changes. The expression adenosine triphosphate (ATP) various HSPs (HSP27, 60, 70, 90) analyzed by immunohistochemical staining, Western...
Photobiomodulation (PBM) using organic light emitting diodes (OLEDs) surface sources have recently been claimed to be the next generation of PBM sources. However, differences between (LEDs) and OLED mechanisms in vitro vivo not well studied. In mouse models were used investigate effects irradiation on cellular function cutaneous wound healing compared LED irradiation. Mice LED- OLED-irradiated groups subjected with $6J/cm^2$ (630 nm), respectively, for 14 days after wounding, some mice...
ABSTRACT Clostridium butyricum DKU-11, a bacterium has been isolated from the stools of breastfed infants near Cheonan-Asan city, Republic Korea, and genome sequence 4,630,814 bp. The GC content is 28.7% total 4,137 coding sequences in two contigs.
Photobiomodulation (PBM) is attracting increased attention in the fields of dermatology and cosmetics. PBM with a variety light parameters has been used widely skin care, but can cause certain types unwanted cells to proliferate skin; this lead tumors, such as papillomas cancers. We constructed mouse model human tumors using DMBA an initiator TPA promoter, confirmed that LEDs wavelength 642 nm (red light) tumor size, epidermal thickness, systemic proinflammatory cytokine levels. These...
A FK506 analogue containing a non-natural starter unit was obtained through mutasynthesis by feeding cultures of Streptomyces sp. KCTC 11604BP fkbO deletion mutant with 3-cyclohexene-1-carboxylic acid. The structure the new compound, 32-dehydroxy-FK506, and its biological activities were determined.
RedP is proposed to initiate undecylprodiginine biosynthesis in Streptomyces coelicolor by condensing an acyl-CoA with malonyl-ACP and homologous FabH that catalyzes the same reaction for initiation of fatty acid biosynthesis. Herein, we report substrate specificities from assays using pairings two substrates (acetyl-CoA isobutyryl-CoA) (malonyl-RedQ malonyl-FabC). activity was observed only a pairing acetyl-CoA malonyl-RedQ, consistent its role initiating formation acetyl-CoA-derived...
FK506 hyper-yielding mutant, called the TCM8594 strain, was made from Streptomyces tsukubaensis NRRL 18488 by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine, ultraviolet irradiation, and sequential resistance selection. production strain improved 45.1-fold (505.4 μg/mL) compared to that of S. (11.2 μg/mL). Among five substrates, wheat bran selected as best solid substrate produce optimum quantities (382.7 μg/g substrate) under solid-state fermentation, process parameters affecting...
Inflammation is the body's physiological response to harmful agents. However, if not regulated properly, inflammation can become pathological. Macrophages are key players in inflammatory process, and modulate immune response. Due side effects of anti-inflammatory drugs, non-pharmaceutical therapies for diseases must be developed. Photobiomodulation a non-invasive therapeutic approach treating certain pathological conditions using light energy. Light-emitting diodes (LEDs) commonly used as...
We identified another tcsABCD genes encoding biosynthesis of the allylmalonyl-CoA in new FK506 producing strain, Streptomyces sp. KCCM 11116P. The functionality is confirmed by gene deletion. To validate these genes, were heterologously expressed hygroscopicus ATCC 14891 using pSE34. resulting strain produced which showed a molecular ion [M+NH4]+ at m/z 821 as verified LC/ESI–MS. address function TcsB pathway, we pursued construction two different combinations: heterologous FabH-like...