A5101464858- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Antifungal resistance and susceptibility
- Biochemical and Structural Characterization
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Topological and Geometric Data Analysis
- Synthesis and Biological Activity
- Microbial Metabolism and Applications
- Optical Coherence Tomography Applications
- Traditional and Medicinal Uses of Annonaceae
- Fungal and yeast genetics research
- Natural product bioactivities and synthesis
- Advanced Fluorescence Microscopy Techniques
- Phytochemical Studies and Bioactivities
- Medical Imaging and Analysis
- Computer Graphics and Visualization Techniques
- Caveolin-1 and cellular processes
- Synthetic Organic Chemistry Methods
- Plant-Microbe Interactions and Immunity
- Botanical Research and Chemistry
- Neuroinflammation and Neurodegeneration Mechanisms
- Wood and Agarwood Research
Seoul National University
2015-2024
New Generation University College
2019
The logical and effective discovery of macrolactams, structurally unique natural molecules with diverse biological activities, has been limited by a lack targeted search methods. Herein, method for macrolactams was devised coupling genomic signature-based PCR screening bacterial DNA library spectroscopic early identification macrolactams. facilitated the efficient selection 43 potential macrolactam-producing strains (3.6% 1,188 screened). amplicons amine-deprotecting enzyme-coding genes were...
Serratiomycin (1) is an antibacterial cyclic depsipeptide, first discovered from a Eubacterium culture in 1998. This compound was initially reported to contain l-Leu, l-Ser, l-allo-Thr, d-Phe, d-Ile, and hydroxydecanoic acid. In the present study, 1 three new derivatives, serratiomycin D1–D3 (2–4), were isolated Serratia sp. strain exoskeleton of long-horned beetle. The planar structures 1–4 elucidated by using mass spectrometry (MS) nuclear magnetic resonance (NMR) spectroscopy. Comparison...
Six new meroterpenoids (1-6), along with arenarol (7), a known rearranged drimane sesquiterpene hydroquinone, were isolated from Dysidea sp. sponge collected the Federated States of Micronesia. On basis results combined spectroscopic analysis, compound 1 was determined to be cyclic ether derivative 7, whereas 2 and 3 assigned as corresponding quinones containing taurine-derived substituents. Compounds 4-6 possess novel tetracyclic skeleton formed by direct linkage between quinone moieties....
The prefibrillar form of soluble amyloid-β (sAβ1-42) impairs synaptic function and is associated with the early phase Alzheimer's disease (AD). We investigated how sAβ1-42 led to presynaptic defects using a quantum dot-based, single particle-tracking method monitor vesicle (SV) trafficking along axons. found that prevented new synapse formation induced by chemical long-term potentiation (cLTP). In cultured rat hippocampal neurons, nanomolar amounts impaired Ca2+ clearance from terminals...
Recently identified human FOXP3
A marine-derived actinomycete (Streptomyces sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain identified using genetic techniques. 16S rDNA sequence of isolate indicated that it most closely related to Streptomyces microflavus. Furthermore, a new chromomycin A9 (1), along with Ap (2), A2 (3), and A3 (4), were isolated from ethyl acetate extract. Their structures determined extensive spectroscopic methods including 1D 2D NMR, HRMS, as well...
Despite being a highly enriched synaptic vesicle (SV) protein and candidate gene for autism, the physiological function of SCAMP5 remains mostly enigmatic. Here, using optical imaging electrophysiological experiments, we demonstrate that plays critical role in release site clearance at active zone. Truncation analysis revealed 2/3 loop domain directly interacts with adaptor 2, this interaction is its clearance. Knockdown (KD) exhibited pronounced depression accompanied by slower recovery SV...
Abstract Various optical clearing approaches have been introduced to meet the growing demand for 3D volume imaging of biological structures. Each has its own strengths but still suffers from low transparency, long incubation time, processing complexity, tissue deformation, or fluorescence quenching, and a single solution that best satisfies all aspects yet developed. Here, we develop OptiMuS, an optimized single-step overcomes shortcomings existing aqueous-based methods provides performance...
Four 2-alkyl-4-hydroxyquinoline derivatives (1⁻4) were isolated from a semisolid rice culture of the marine-derived actinomycete Streptomyces sp. MBTG13. The structures these compounds elucidated by combination spectroscopic methods, and their data in good agreement with previous reports. Compounds 1 2 exhibited weak to moderate antibacterial activity against pathogenic bacteria. Unexpectedly, we found that compound acted as potent inhibitor hyphal growth induction dimorphic fungus Candida...
Six new bis(indole) alkaloids (1–6) along with eight known ones of the topsentin class were isolated from a Spongosorites sp. sponge Korea. Based on results combined spectroscopic analyses, structures spongosoritins A–D (1–4) determined to possess 2-methoxy-1-imidazole-5-one core connecting indole moieties, and these linked by linear urea bridge for spongocarbamides A (5) B (6). The absolute configurations assigned electronic circular dichroism (ECD) computation. compounds exhibited moderate...
Streptomyces sp. GET02.ST and Achromobacter GET02.AC were isolated together from the gut of wharf roach, Ligia exotica, inhabiting intertidal zone west coast Korea. The co-cultivation these two strains significantly induced production new metabolites, ligiamycins A (1) B (2), which barely detected in single culture GET02.ST. planar structures (2) elucidated as decalins coupled with amino-maleimides by analysis various spectroscopic data, including nuclear magnetic resonance (NMR),...
Two nitrogenous metabolites, bacillimide (1) and bacillapyrrole (2), were isolated from the culture broth of marine-derived actinomycete Streptomyces bacillaris. Based on results combined spectroscopic chemical analyses, structure was determined to be a new cyclopenta[c]pyrrole-1,3-dione bearing methylsulfide group, while previously reported (2) fully characterized for first time as pyrrole-carboxamide an alkyl sulfoxide side chain. Bacillimide exerted moderate (IC50 = 44.24 μM) weak 190.45...
Seven alkaloidal compounds (2–8) and one polyketide (1) were isolated from a semisolid rice culture of the marine-derived fungus Aspergillus sp. F452. Structures elucidated based on spectroscopic data comparisons with previously reported data. The displayed weak to moderate inhibitory activities against Staphylococcus aureus-derived sortase A (SrtA) without affecting cell viability. Aspermytin strongly inhibited SrtA activity, an IC50 value 146.0 μM, significantly reduced bacterial adherence...
Dendritic spines are dynamic, submicron-scale protrusions on neuronal dendrites that receive inputs. Morphological changes in the dendritic spine often reflect alterations physiological conditions and indicators of various neuropsychiatric conditions. However, owing to highly dynamic heterogeneous nature spines, accurate measurement objective analysis morphology major challenges neuroscience research. Most conventional approaches for analyzing based two-dimensional (2D) images, which barely...
Four epipolythiodioxopiperazine fungal metabolites (1-4) isolated from the sponge-derived Aspergillus quadrilineatus FJJ093 were evaluated for their capacity to inhibit isocitrate lyase (ICL) in glyoxylate cycle of Candida albicans. The structures these compounds elucidated using spectroscopic techniques and comparisons with previously reported data. We found secoemestrin C (1) (an epitetrathiodioxopiperazine derivative) be a potent ICL inhibitor, an inhibitory concentration 4.77 ± 0.08 μM....
Isocadiolides A-H (1-8) and cadiolide N (9), new polybrominated aromatic compounds, were isolated from a Korean Synoicum sp. ascidian. On the basis of results extensive spectroscopic analyses, these compounds possessed tris-bromohydroxyphenyl moieties as common structural motif, while their cores varied [cyclopentenedione (1-5), dihydrofuran (6 7), pyranone (8), furanone (9)], reflecting different extents rearrangement oxidation. Several exhibited weak antibacterial activities moderate...
Streptococcus mutans, an important Gram-positive pathogen in dental caries, uses sortase A (SrtA) to anchor surface proteins the bacterial cell wall, thereby promoting biofilm formation and attachment tooth surface.Based on activity-guided separation, inhibitors of S. mutans SrtA were isolated from Juniperus chinensis identified through combined spectroscopic analysis. Further effects inhibitor evaluated aggregation, adherence formation.Six compounds (1-6) dried heartwood J. chinensis. novel...
Candida albicans hyphal formation is inhibited by a quorum-sensing molecule, farnesoic acid, which accumulates in the medium as cells proliferate. We recently showed that Pho81 essential for inhibition of growth acid. Here, we describe newly identified regulator, Hot1, increases expression PHO81. The binding site Hot1 PHO81 promoter region was DNase I protection assay. hot1Δ mutant grows extensively filaments. Furthermore, and repression major signaling pathway components response to acid...
Src homology 3-domain growth factor receptor-bound 2-like interacting protein 1 (SGIP1), originally known as a regulator of energy homeostasis, was later found to be an ortholog Fer/Cip4 domain-only (FCHo) proteins and function during endocytosis. SGIP1α is longer splicing variant in mouse brains that contains additional regions the membrane phospholipid-binding domain (MP) C-terminal region, but functional consequences with or without between SGIP1 remain elusive. Moreover, many previous...
nArgBP2, a candidate gene for intellectual disability, is postsynaptic protein critical dendritic spine development and morphogenesis, its knockdown (KD) in developing neurons severely impairs spine-bearing excitatory synapse formation. Surprisingly, nArgBP2 KD mature did not cause morphological defects the existing spines at rest, raising questions of how it functions neurons. We found that unlike inaction completely inhibited enlargement during chemically induced long-term potentiation...
Sortase A (SrtA) is a cysteine transpeptidase that binds to the periplasmic membrane and plays crucial role in attaching surface proteins, including staphylococcal protein (SpA), peptidoglycan cell wall. Six pentacyclic polyketides (1–6) were isolated from marine sponge Xestospongia sp., their structures elucidated using spectroscopic techniques by comparing them previously reported data. Among them, halenaquinol (2) was found be most potent SrtA inhibitor, with an IC50 of 13.94 μM (4.66...
Dendritic spines are submicron scale protrusions on neuronal dendrites that form the postsynaptic sites of excitatory inputs. The morphological changes dendritic reflect alterations in physiological conditions and further indicators various neuropsychiatric conditions. However, due to highly dynamic heterogeneous nature spines, accurate measurement object analysis spine morphology is a major challenge neuroscience research. Here, we propose an interactive 3D system displays rendering plots...