A5007597056- Dietary Effects on Health
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Cellular transport and secretion
- Diet and metabolism studies
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Gut microbiota and health
- Circadian rhythm and melatonin
- Neurological Disease Mechanisms and Treatments
- Erythrocyte Function and Pathophysiology
- interferon and immune responses
- Barrier Structure and Function Studies
- Molecular Biology Techniques and Applications
- Adenosine and Purinergic Signaling
- Circular RNAs in diseases
- Advanced Neural Network Applications
- Neurological disorders and treatments
- Glycosylation and Glycoproteins Research
- Cell Image Analysis Techniques
- Digital Radiography and Breast Imaging
- AI in cancer detection
- Heme Oxygenase-1 and Carbon Monoxide
- Folate and B Vitamins Research
University of Wisconsin–Madison
2022-2025
Neurological Surgery
2022-2025
Highland Community College - Illinois
2025
Seoul National University
2016-2021
Korea Institute of Brain Science
2019
Korea Institute of Science and Technology
2019
Preconditioning by intermittent fasting is linked to improved cognition and motor function, enhanced recovery after stroke. Although the duration of was shown elicit different levels neuroprotection ischemic stroke, impact time with respect circadian cycles remains unexplored.
FTO (fat mass and obesity-associated protein) demethylates
Intermittent fasting (IF) is known to induce significant ischemic tolerance. Diet a major proponent of gut microbiota, and microbial dysbiosis plays role in post-stroke brain damage. Hence, we currently evaluated whether IF-mediated tolerance mediated by microbiota. Additionally, circadian cycle modulate post-ischemic outcomes, thus further if microbiota would be influenced prophylactic IF during the inactive phase (fasting daytime; IIF) or active nighttime; AIF). The AIF, but not IIF,...
We previously showed that knockdown or deletion of Fos downstream transcript (FosDT; a stroke-induced brain-specific long noncoding RNA) is neuroprotective. presently tested the therapeutic potential FosDT siRNA in rodents subjected to transient middle cerebral artery occlusion (MCAO) using Stroke Treatment Academic Industry Roundtable criteria, including sex, age, species, and comorbidity. (IV) given at 30 min reperfusion significantly improved motor function recovery (rotarod test, beam...
Significance Recent studies have implicated postsynaptic scaffolding protein synapse-associated 90/postsynaptic density 95-associated 3 (SAPAP3) or Shank3 in mood disorders such as bipolar disorder, autism, and obsessive-compulsive disorders. Neural Abelson-related gene-binding 2 (nArgBP2) is a binding partner of both SAPAP3 Shank3, its genetic deletion mice leads to manic/bipolar-like behavior resembling symptoms disorder. Remarkably, nothing known about the synaptic function nArgBP2...
Proper sorting of exocytosed synaptic vesicle (SV) proteins into individual SVs during endocytosis is the utmost importance for fidelity subsequent neurotransmission. Recent studies suggest that each SV protein sorted by its own dedicated adaptors as well association between proteins. The SH3-containing GRB2-like 3-interacting 1 (SGIP1), an ortholog Fer/Cip4 homology domain-only (FCHo) proteins, contains a μ-homology domain (μHD) and binds AP-2 Eps15, thus functioning endocytic regulator...
The complex pathophysiology of post-traumatic brain damage might need a polypharmacological strategy with combination drugs that target multiple, synergistic mechanisms. We currently tested apocynin (curtails formation reactive oxygen species), tert-butylhydroquinone (promotes disposal and salubrinal (prevents endoplasmic reticulum stress) following moderate traumatic injury (TBI) induced by controlled cortical impact in adult mice. Adult mice both sexes treated the above tri-combo showed...
Quantitative analysis of cell nuclei in microscopic images is an essential yet challenging source biological and pathological information. The major challenge accurate detection segmentation densely packed acquired under a variety conditions. Mask R-CNN-based methods have achieved state-of-the-art nucleus segmentation. However, the current pipeline requires fully annotated training images, which are time consuming to create sometimes noisy. Importantly, often appear similar within same...
Src homology 3-domain growth factor receptor-bound 2-like interacting protein 1 (SGIP1), originally known as a regulator of energy homeostasis, was later found to be an ortholog Fer/Cip4 domain-only (FCHo) proteins and function during endocytosis. SGIP1α is longer splicing variant in mouse brains that contains additional regions the membrane phospholipid-binding domain (MP) C-terminal region, but functional consequences with or without between SGIP1 remain elusive. Moreover, many previous...
Fat mass and obesity-associated protein (FTO) demethylates N 6 -methyladenosine (m A), which is a critical epitranscriptomic regulator of neuronal function. We previously reported that ischemic stroke induces m A hypermethylation with simultaneous decrease in FTO expression the neurons. Currently, we evaluated functional significance restoring an adeno-associated virus 9 (AAV9), thus reducing methylation post-stroke brain damage. Adult male female C57BL/6J mice were injected AAV9...
Epigenetic mechanisms have been shown to play a major role in the progression of stroke pathophysiology. The ten eleven translocases (TETs) are enzymes involved generating DNA hydroxymethylcytosine (5hmC), brain enriched epigenetic modification that is associated with transcriptional activation and neuroprotection. We previously TET3 isoform may be promoting endogenous neuroprotective pathways following injury. In current study, we investigated activity after by examining functional...