A5045339460- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Neurological Disease Mechanisms and Treatments
- Circular RNAs in diseases
- Bacterial Identification and Susceptibility Testing
- Neuroinflammation and Neurodegeneration Mechanisms
- Dietary Effects on Health
- Streptococcal Infections and Treatments
- Respiratory viral infections research
- Clinical Laboratory Practices and Quality Control
- Cell Adhesion Molecules Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Vitamin C and Antioxidants Research
- Conducting polymers and applications
- Acute Ischemic Stroke Management
- Cystic Fibrosis Research Advances
- Diet and metabolism studies
- Vitamin K Research Studies
- COVID-19 Impact on Reproduction
- Barrier Structure and Function Studies
- Pregnancy and Medication Impact
- Artificial Intelligence in Healthcare and Education
University of Wisconsin–Madison
2018-2025
Baylor College of Medicine
2022-2025
Texas Children's Hospital
2022-2025
Neurological Surgery
2018-2025
Background and Purpose— Adenosine in many types of RNAs can be converted to m 6 A (N -methyladenosine) which is a highly dynamic epitranscriptomic modification that regulates RNA metabolism function. Of all organs, the brain shows highest abundance methylation RNAs. As recent studies showed promotes cell survival after adverse conditions, we currently evaluated effect stroke on cerebral mRNAs lncRNAs. Methods— Adult C57BL/6J mice were subjected transient middle artery occlusion. In...
Ischemic stroke, which is caused by a clot that blocks blood flow to the brain, can be severely disabling and sometimes fatal. We previously showed transient focal ischemia in rat model induces extensive temporal changes expression of cerebral microRNAs, with sustained decrease abundance miR-7a-5p (miR-7). Here, we evaluated therapeutic efficacy miR-7 mimic oligonucleotide after rodents according Stroke Treatment Academic Industry Roundtable (STAIR) criteria. Rodents were injected locally or...
Preconditioning by intermittent fasting is linked to improved cognition and motor function, enhanced recovery after stroke. Although the duration of was shown elicit different levels neuroprotection ischemic stroke, impact time with respect circadian cycles remains unexplored.
FTO (fat mass and obesity-associated protein) demethylates
Transient focal ischemia decreased microRNA-7 (miR-7) levels, leading to derepression of its major target α-synuclein (α-Syn) that promotes secondary brain damage. Circular RNA CDR1as is known regulate miR-7 abundance and function. Hence, we currently evaluated functional significance after ischemia. middle cerebral artery occlusion (MCAO) in adult mice significantly downregulated both levels the peri-infarct cortex between 3 72 h reperfusion. Interestingly, neither pri-miR-7a nor 7b was...
Intermittent fasting (IF) is known to induce significant ischemic tolerance. Diet a major proponent of gut microbiota, and microbial dysbiosis plays role in post-stroke brain damage. Hence, we currently evaluated whether IF-mediated tolerance mediated by microbiota. Additionally, circadian cycle modulate post-ischemic outcomes, thus further if microbiota would be influenced prophylactic IF during the inactive phase (fasting daytime; IIF) or active nighttime; AIF). The AIF, but not IIF,...
Similar symptoms between viral and bacterial diseases often make diagnosis difficult. This study assessed the clinical performance of newly cleared whole-blood Bacterial versus Viral Score assay in our pediatric cohort to previously validated serum emergency department physician diagnosis. shows excellent agreement (R = 0.997) with has great diagnostic accuracy when compared
The 5-hydroxymethylcytosine (5hmC) epigenetic modification is highly enriched in the CNS and a critical modulator of neuronal function development. We found that cortical 5hmC was enhanced from 5 min to three days reperfusion following focal ischemia adult mice. Blockade 5hmC-producing enzyme ten-eleven translocase 3 (TET3) increased edema, infarct volume, motor impairments. To determine mechanism by which TET3 provides ischemic neuroprotection, we assessed genomic regions where modulates...
The role of tenascin-C (TNC) in ischemic stroke pathology is not known despite its prognostic association with cerebrovascular diseases. Here, we investigated the effect TNC knockdown on post-stroke brain damage and putative mechanism action adult mice both sexes. Male female C57BL/6 were subjected to transient middle cerebral artery occlusion injected (i.v.) either siRNA or a negative (non-targeting) at 5 min after reperfusion. Motor function (beam walk rotarod tests) was assessed between...
Background and Purpose: Stroke induces the expression of several long noncoding RNAs in brain. However, their functional significance poststroke outcome is poorly understood. We recently observed that a brain-specific RNA called Fos downstream transcript (FosDT) induced rapidly rodent brain following focal ischemia. Using FosDT knockout rats, we presently evaluated role damage. Methods: rats were generated using CRISPR-Cas9 genome editing on Sprague-Dawley background. Male female −/− +/+...
Ubiquitination by Nedd4 (neuronally expressed developmentally downregulated 4) family of HECT type E3 ligases plays a key role in degrading misfolded and damaged proteins, its disruption leads to neurodegeneration. Parkinson's disease-causing protein α-Synuclein (α-Syn) is ubiquitinated the degraded endosomes. Nedd4l only homolog that showed upregulation post-stroke surviving cortical neurons where it correlated with neuroprotection. We tested after stroke subjecting −/− mice transient...
The complex pathophysiology of post-traumatic brain damage might need a polypharmacological strategy with combination drugs that target multiple, synergistic mechanisms. We currently tested apocynin (curtails formation reactive oxygen species), tert-butylhydroquinone (promotes disposal and salubrinal (prevents endoplasmic reticulum stress) following moderate traumatic injury (TBI) induced by controlled cortical impact in adult mice. Adult mice both sexes treated the above tri-combo showed...
Abstract Objective Cystic Fibrosis Foundation guidelines recommend annual diabetes screening by oral glucose tolerance test (OGTT) in pediatric patients with cystic fibrosis (CF) starting at the age of 10 years. Adherence to these proves be challenging, and nationwide rates are still considered suboptimal. The aim this study was assess improve our large center. Methods A 4-year retrospective audit OGTT completion among CF ≥10 years who not yet diagnosed conducted. collaborative working group...