A5074949242- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Cardiomyopathy and Myosin Studies
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Neural dynamics and brain function
- Traumatic Brain Injury and Neurovascular Disturbances
- Force Microscopy Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Morphological variations and asymmetry
- Medical Imaging and Analysis
- Topological and Geometric Data Analysis
- Advanced Vision and Imaging
- Neurogenesis and neuroplasticity mechanisms
- interferon and immune responses
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- Traumatic Brain Injury Research
- Cell Image Analysis Techniques
The University of Tokyo
2012-2022
Centre for Research in Engineering Surface Technology
2015
Center for Responsible Travel
2015
Centre de Recherche en Économie et Statistique
2015
Dendritic morphogenesis and formation of synapses at appropriate dendritic locations are essential for the establishment proper neuronal connectivity. Recent imaging studies provide evidence stabilization dynamic distal branches dendrites by addition new synapses. However, molecules involved in both growth suppression synapse maturation remain to be identified. Here we report two distinct functions doublecortin-like kinases, chimeric proteins containing a microtubule-binding domain kinase...
Abstract Dendritic spines are the postsynaptic sites that receive most of excitatory synaptic inputs, and thus provide structural basis for function. Here, we describe an accurate method measurement analysis spine morphology based on structured illumination microscopy (SIM) computational geometry in cultured neurons. Surface mesh data converted from SIM images were comparable to reconstructed electron microscopic images. Dimensional reduction machine learning applied large sets enabled...
Synaptic remodelling coordinated with dendritic growth is essential for proper development of neural connections. After establishment synaptic contacts, junctions are thought to become stationary and provide fixed anchoring points further growth. However, the possibility active translocation synapses along protrusions, guide arrangement distribution, has not yet been fully investigated. Here we show that immature dendrites γ-aminobutyric acid-positive interneurons form long protrusions these...
Ramified, polarized protoplasmic astrocytes interact with synapses via perisynaptic astrocyte processes (PAPs) to form tripartite synapses. These astrocyte-synapse interactions mutually regulate their structures and functions. However, molecular mechanisms for synapse formation remain elusive. We developed an in vitro co-culture system mouse neurons that induced ramifications PAP formation. Co-cultured were required a neuronal activity-dependent manner, synaptically-released glutamate...
Abstract Traumatic brain injury (TBI) is one of the major causes death and disability. Multiple animal models have been developed to explore therapeutic targets for TBI. However, heterogeneity pathophysiology obstructs discovery targets. To facilitate understanding TBI pathophysiology, each element neuronal glial responses should be studied separately. We focused on synapse remodeling which plays an important role in recovery from a new method, afferent elimination, analyzing after selective...
Dendritic spines are submicron scale protrusions on neuronal dendrites that form the postsynaptic sites of excitatory inputs. The morphological changes dendritic reflect alterations in physiological conditions and further indicators various neuropsychiatric conditions. However, due to highly dynamic heterogeneous nature spines, accurate measurement object analysis spine morphology is a major challenge neuroscience research. Here, we propose an interactive 3D system displays rendering plots...
Dynamic organization of postsynaptic density (PSD) and cytoskeleton in dendritic spines are thought to be crucial for excitatory synapse development [ 1 ]. Actin network has prominent roles determining spine morphology anchoring proteins. A sub-population the dynamic MTs transiently invades into 2 ], may contribute critical events 3 ACF7/MACF1 is a cross-linking protein that containing an actin filament binding domain MT within single molecule. ACF7 previously- identified as candidate...