A5100635866- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Plant biochemistry and biosynthesis
- Ginseng Biological Effects and Applications
- Antifungal resistance and susceptibility
- Peroxisome Proliferator-Activated Receptors
- Pharmacological Effects of Natural Compounds
- Plant tissue culture and regeneration
- Asymmetric Hydrogenation and Catalysis
- Biochemical and Structural Characterization
- Toxoplasma gondii Research Studies
- Molecular Biology Techniques and Applications
- Plant Stress Responses and Tolerance
- HIV Research and Treatment
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Urinary and Genital Oncology Studies
- ATP Synthase and ATPases Research
- Nuclear materials and radiation effects
- Asthma and respiratory diseases
- Mycotoxins in Agriculture and Food
- Phytochemical Studies and Bioactivities
- Diet, Metabolism, and Disease
- Microbial Metabolism and Applications
University of Wisconsin–Madison
2022-2024
Seoul National University
2019-2023
Korea Research Institute of Bioscience and Biotechnology
2017-2021
Busan Institute of Science and Technology
2019
Korea Advanced Institute of Science and Technology
1997-2015
Intelligent Synthetic Biology Center
2014
Yeungnam University
2009
Sogang University
1989-1990
Ginseng is a medicinal herb that requires cultivation under shade conditions, typically for 4-6 years, before harvesting. The principal components of ginseng are ginsenosides, glycosylated tetracyclic terpenes. Dammarene-type ginsenosides classified into two groups, protopanaxadiol (PPD) and protopanaxatriol (PPT), based on their hydroxylation patterns, further diverge to diverse through differential glycosylation. Three early enzymes, dammarenediol-II synthase (DS) P450 (PPDS) (PPTS), have...
Marinobazzanan (1), a new bazzanane-type sesquiterpenoid, was isolated from marine-derived fungus belonging to the genus Acremonium. The chemical structure of 1 elucidated using NMR and mass spectroscopic data, while relative configurations were established through analysis NOESY data. absolute determined by modified Mosher's method as well vibrational circular dichroism (VCD) spectra calculation it 6R, 7R, 9R, 10R. It found that compound not cytotoxic human cancer cells, including A549...
The ginsenoside Rg2(S), which is one of the pharmaceutical components ginseng, known to have neuroprotective, anti-inflammation, and anti-diabetic effects. However, usage Rg2(S) restricted owing small amounts found in white red ginseng. To enhance production as a 100 gram unit with high specificity, yield, purity, an enzymatic bioconversion method was developed adopt recombinant glycoside hydrolase (BglPC28), ginsenoside-transforming β-glucosidase from Pseudonocardia sp. strain Gsoil 1536....
Adiponectin is an adipocyte-derived cytokine having insulin-sensitizing activity. During the phenotypic screening of secondary metabolites derived from marine fungus Aspergillusterreus, a poly cyclin-dependent kinase (CDK) inhibitor butyrolactone I affecting CDK1 and CDK5 was discovered as potent adiponectin production-enhancing compound in adipogenesis model human bone marrow-derived mesenchymal stem cells (hBM-MSCs). inhibitors exhibit activities by suppressing phosphorylation peroxisome...
Foam cell formation is the most important process in atherosclerosis, and low density lipoprotein oxidation by reactive oxygen species (ROS) key step conversion of macrophages to foam cells. This study reveals control mechanism gene for NADPH oxidase 1 (Nox1), which produces ROS cells stimulating TLR4. Treatment TLR4 agonist LPS stimulated production ROS-mediated macrophage conversion. LPS-induced could be abrogated pretreatment with N-acetyl cysteine or apocynin. increased Nox1 promoter...
Filamentous fungi produce numerous uncharacterized natural products (NPs) that are often challenging to characterize because of cryptic expression in laboratory conditions. Previously, we have successfully isolated novel NPs by expressing fungal artificial chromosomes (FACs) from a variety species into
Abstract Aspergillus fumigatus is the leading causative agent of life-threatening invasive aspergillosis in immunocompromised individuals. One antifungal class used to treat infections fungistatic echinocandins, semisynthetic drugs derived from naturally occurring fungal lipopeptides. By inhibiting beta-1,3-glucan synthesis, echinocandins cause both stunting hyphal growth and repeated fungicidal lysis apical tip compartments. Here, we uncover an endogenous mechanism echinocandin tolerance A....
Four new peptides were isolated from the culture broths of marine-derived fungi Aspergillus allahabadii and A. ochraceopetaliformis. Based on results chemical spectroscopic analyses, two compounds (1 2) determined to be cyclopentapeptides, while those ochraceopetaliformis a structurally-related cyclodepsihexapeptide (3) its linear analog (4). In addition presence D-amino acid residue, almost reversed sequence in 3 4, relative 1 2, is notable. These exhibited moderate inhibition against...
Six new phenalenone derivatives (1⁻6), along with five known compounds (7⁻11) of the herqueinone class, were isolated from a marine-derived fungus Penicillium sp. The absolute configurations these assigned based on chemical modifications and their specific rotations. 4-Hydroxysclerodin (6) an acetone adduct triketone (7) exhibited moderate anti-angiogenetic anti-inflammatory activities, respectively, while ent-peniciherqueinone (1) isoherqueinone (9) abilities to induce adipogenesis without...
Lipases have been utilized industrially to produce biodiesel, oleochemicals, and pharmaceuticals. Many efforts such as metagenomics, directed evolution, enzyme immobilization devoted enhance the lipase activity. Here, we designed a recombinant lipase, NKC-M37-MAT, that was generated by incorporating an N-terminal amphipathic peptide (NKC) C-terminal coiled-coil (MAT) into Photobacterium lipolyticum M37 lipase. The hydrophobic face of NKC improve accessibility (Km), catalytic efficiency...
Six new bis(indole) alkaloids (1–6) along with eight known ones of the topsentin class were isolated from a Spongosorites sp. sponge Korea. Based on results combined spectroscopic analyses, structures spongosoritins A–D (1–4) determined to possess 2-methoxy-1-imidazole-5-one core connecting indole moieties, and these linked by linear urea bridge for spongocarbamides A (5) B (6). The absolute configurations assigned electronic circular dichroism (ECD) computation. compounds exhibited moderate...
The oat (Avena sativa L.) is a grain of the Poaceae grass family and contains many powerful anti-oxidants, including avenanthramides as phenolic alkaloids with anti-inflammatory, anti-oxidant, anti-itch, anti-irritant, anti-atherogenic activities. Here, treatment germinating oats methyl jasmonate (MeJA) or abscisic acid (ABA) resulted in 2.5-fold (582.9 mg/kg FW) 2.8-fold (642.9 increase avenanthramide content, respectively, relative to untreated controls (232.6 FW). Moreover, MeJA ABA...
Alcohol oxidases (AOXs) are ecologically important enzymes that facilitate a number of plant-fungal interactions. Within Ascomycota they primarily associated with methylotrophy, as peroxisomal AOX catalysing the conversion methanol to formaldehyde in methylotrophic yeast. In this study we demonstrate orthologues phylogenetically conserved proteins common genomes nonmethylotrophic, plant-associating fungi. Additionally, highly expressed during infection range diverse pathosystems. To role...
Three new bianthraquinones, alterporriol Z1-Z3 (1-3), along with three known compounds of the same structural class, were isolated from culture broth a marine-derived Stemphylium sp. fungus. Based upon results spectroscopic analyses and ECD measurements, structures determined to be 6-6'- (1 2) 1-5'- (3) C-C connected pseudo-dimeric anthraquinones, respectively. meroterpenoids, tricycloalterfurenes E-G (7-9), together bianthraquinones fungal broth, structurally elucidated by combined methods....
Two nitrogenous metabolites, bacillimide (1) and bacillapyrrole (2), were isolated from the culture broth of marine-derived actinomycete Streptomyces bacillaris. Based on results combined spectroscopic chemical analyses, structure was determined to be a new cyclopenta[c]pyrrole-1,3-dione bearing methylsulfide group, while previously reported (2) fully characterized for first time as pyrrole-carboxamide an alkyl sulfoxide side chain. Bacillimide exerted moderate (IC50 = 44.24 μM) weak 190.45...
Seven alkaloidal compounds (2–8) and one polyketide (1) were isolated from a semisolid rice culture of the marine-derived fungus Aspergillus sp. F452. Structures elucidated based on spectroscopic data comparisons with previously reported data. The displayed weak to moderate inhibitory activities against Staphylococcus aureus-derived sortase A (SrtA) without affecting cell viability. Aspermytin strongly inhibited SrtA activity, an IC50 value 146.0 μM, significantly reduced bacterial adherence...
Colorectal cancer (CRC) is a common and intractable malignancy with high mortality risk. Conventional chemotherapeutics are effective for patients early stage CRC, but the majority of deaths CRC linked to acquired drug resistance or metastasis occurrence. Asperphenin B (1), lipopeptidyl benzophenone isolated from marine-derived Aspergillus sp. fungus, reportedly possesses antiproliferative activity against cells. However, its antitumor underlying molecular mechanisms remain unexplored. In...
Abstract The obligate intracellular parasite Toxoplasma gondii can infect and replicate in any warm-blooded cell tested to date, but much of our knowledge about T. biology comes from just one host type: human foreskin fibroblasts (HFFs). To expand host-parasite lipid interactions, we studied intestinal epithelial cells, the first site contact following oral infection exclusive sexual development feline hosts. We found that highly metabolic Caco-2 cells are permissive growth even when treated...
The obligate intracellular parasite
Four epipolythiodioxopiperazine fungal metabolites (1-4) isolated from the sponge-derived Aspergillus quadrilineatus FJJ093 were evaluated for their capacity to inhibit isocitrate lyase (ICL) in glyoxylate cycle of Candida albicans. The structures these compounds elucidated using spectroscopic techniques and comparisons with previously reported data. We found secoemestrin C (1) (an epitetrathiodioxopiperazine derivative) be a potent ICL inhibitor, an inhibitory concentration 4.77 ± 0.08 μM....
The poisonous European mushroom Amanita phalloides (the "death cap") is invading California. Whether the death caps' toxic secondary metabolites are evolving as it invades unknown. We developed a bioinformatic pipeline to identify MSDIN genes underpinning toxicity and probed 88 cap genomes from an invasive Californian population range, discovering previously unsuspected diversity of MSDINs made up both core accessory elements. Each individual possesses unique suite MSDINs, toxin...