A5102997153- Ginseng Biological Effects and Applications
- Pharmacological Effects of Natural Compounds
- Plant tissue culture and regeneration
- Natural product bioactivities and synthesis
- Polysaccharides and Plant Cell Walls
- Genomics and Phylogenetic Studies
- Telomeres, Telomerase, and Senescence
- Dermatology and Skin Diseases
- Toxin Mechanisms and Immunotoxins
- Mycobacterium research and diagnosis
- Plant Pathogenic Bacteria Studies
- Phytochemical compounds biological activities
- Tendon Structure and Treatment
- Plant Disease Resistance and Genetics
- Plant biochemistry and biosynthesis
- Microbial infections and disease research
- Immune Cell Function and Interaction
- Microbial Metabolites in Food Biotechnology
- Wound Healing and Treatments
- Sphingolipid Metabolism and Signaling
- Plant Molecular Biology Research
- Cytomegalovirus and herpesvirus research
- Neuroinflammation and Neurodegeneration Mechanisms
- Dermatologic Treatments and Research
- Phytochemistry and Bioactivity Studies
Intelligent Synthetic Biology Center
2016-2022
Korea Advanced Institute of Science and Technology
2010-2022
A new beta-glucosidase from a novel strain of Terrabacter ginsenosidimutans (Gsoil 3082(T)) obtained the soil ginseng farm was characterized, and gene, bgpA (1,947 bp), cloned in Escherichia coli. The enzyme catalyzed conversion ginsenoside Rb1 {3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol} to more pharmacologically active rare ginsenosides gypenoside XVII...
A novel β-glucosidase (BglPm) was identified from Paenibacillus mucilaginosus KCTC 3870T which has ginsenoside converting activity. The gene, termed bglPm, consists of 1,260 bp and belongs to glycoside hydrolase family 1 (GH1). After being overexpressed purified Escherichia coli, the enzymatic properties BglPm were investigated. enzyme exhibited an optimal activity at 45°C pH 7.5 showed high bioconversion ability for major Rb1 Rd into F2. Thus, it used mass production relatively pure F2...
Here, we isolated and characterized a new ginsenoside-transforming β-glucosidase (BglQM) from Mucilaginibacter sp. strain QM49 that shows biotransformation activity for various major ginsenosides. The gene responsible this activity, bglQM, consists of 2,346 bp is predicted to encode 781 amino acid residues. This enzyme has molecular mass 85.6 kDa. Sequence analysis BglQM revealed it could be classified into glycoside hydrolase family 3. was overexpressed in Escherichia coli BL21(DE3) using...
Minor ginsenosides, such as compound K, Rg3(S), which can be produced by deglycosylation of ginsenosides Rb1, showed strong anti-cancer effects. However, the anticancer effects gypenoside LXXV, is one deglycosylated shapes ginsenoside still unknown due to rarity its content in plants. Here, we cloned and characterized a novel ginsenoside-transforming β-glucosidase (BglG167b) derived from Microbacterium sp. Gsoil 167 efficiently hydrolyze XVII into applied it production LXXV at gram-scale...
Use of recombinant glycosidases is a promising approach for the production minor ginsenosides, e.g., Compound K (CK) and F1, which have potential applications in food industry. However, application these enzymes food-grade preparation ginsenosides are limited by lack suitable expression hosts low productivity. In this study, Corynebacterium glutamicum ATCC13032, GRAS strain that has been used extensively industrial-grade additives foodstuffs, was employed to express novel β-glucosidase...
Ginseng has been shown to produce a cognitive improvement effect. The key molecular components in ginseng that pharmacological effects are ginsenosides. Previous studies reported memory effect of few major However, the identity specific minor ginsenosides mediating such function remains unknown. Here, we report ginsenoside F1 improves APPswe/PSEN1dE9 (APP/PS1) double-transgenic Alzheimer's disease (AD) model mice. After 8-wk oral administration jelly, observed spatial working memory, but not...
The anthracycline antibiotic doxorubicin is commonly used antineoplastic drug in breast cancer treatment. Like most chemotherapy, does not selectively target tumorigenic cells with high proliferation rate and often causes serve side effects. In the present study, we demonstrated cellular senescence associated secretory phenotype (SASP) of both tumor cell MDA-MB-231 normal epithelial MCF-10A induced by clinical dose (100 nM). Senescence was confirmed flattened morphology, increased level beta...
A gram-reaction-positive, rod-shaped, spore-forming bacterium, designated Gsoil 1105(T), was isolated from soil of a ginseng field in Pocheon Province South Korea and characterized order to determine its taxonomic position. Comparative analysis the 16S rRNA gene sequence showed that isolate belongs Bacillales, showing highest level similarity with respect Tumebacillus permanentifrigoris Eur1 9.5(T) (94.6 %). The phylogenetic distances other described species validly published names within...
The ginsenoside Rg2(S), which is one of the pharmaceutical components ginseng, known to have neuroprotective, anti-inflammation, and anti-diabetic effects. However, usage Rg2(S) restricted owing small amounts found in white red ginseng. To enhance production as a 100 gram unit with high specificity, yield, purity, an enzymatic bioconversion method was developed adopt recombinant glycoside hydrolase (BglPC28), ginsenoside-transforming β-glucosidase from Pseudonocardia sp. strain Gsoil 1536....
Ginsenosides are known to have various highly pharmacological activities, such as anti-cancer and anti-inflammatory effects. However, the search for most effective ginsenosides against pathogenesis of atopic dermatitis (AD) study effects on specific cytokines involved in AD remain unclear. In this study, ginsenoside Rh2 was shown exert action thymic stromal lymphopoietin (TSLP) interleukin 8 tumor necrosis factor-alpha polyinosinic: polycytidylic acid induced normal human keratinocytes by...
The ginsenoside Rh2, a pharmaceutically active component of ginseng, is known to have anticancer and antitumor effects. However, white ginseng red extremely low concentrations Rh2 or Rh2-Mix [20(S)-Rh2, 20(R)-Rh2, Rk2, Rh3]. To enhance the production food-grade an edible enzymatic bioconversion technique was developed adopting GRAS host strains. A β-glucosidase (BglPm), which has conversion ability, expressed in three strains (Corynebacterium glutamicum, Saccharomyces cerevisiae Lactococus...
A Gram-positive, spore-forming, aerobic actinomycete, strain Gsoil 519T, was isolated from soil of a ginseng field Pocheon province in South Korea. The closest phylogenetic relatives were Streptomyces marinus Sp080513GE-26T (97.94 % 16S rRNA gene sequence similarity), albiaxialis NRRL B-24327T (97.84 %), albus subsp. DSM 40313T almquistii NBRC 13015T (97.81 gibsonii 15415T rangoonensis 13078T sodiiphilus YIM 80305T (97.77 %) and flocculus 13041T (97.67 %). G+C content the genomic DNA 71.8...
Cutaneous wound healing is a well-orchestrated event in which many types of cells and growth factors are involved restoring the barrier function skin. In order to identify whether ginsenosides, main active components Panax ginseng, promote healing, proliferation migration activities 15 different ginsenosides were tested by MTT assay scratched closure assay. Among gypenoside LXXV (G75) showed most potent effects. Thus, this study aimed investigate effects G75 on vivo characterize associated...
Background: Ginsenosides, triterpene saponins of Panax species, are considered the main active ingredients responsible for various pharmacological activities. Herein, a new protopanaxatriol-type ginsenoside called “ginsenoside MT1” is described; it was accidentally found among enzymatic conversion products Re. Method: We analyzed mechanism and that recombinant β-glucosidase (MT619) transglycosylated outer rhamnopyranoside Re at C-6 position to glucopyranoside C-20. The production MT1 by...
Cascade reaction systems, such as protein fusion and synthetic scaffold can both spatially control the metabolic flux boost productivity of multistep enzymatic reactions. Despite many efforts to generate proteins, this task remains challenging due limited expression complex enzymes. Therefore, we developed a novel system that bypasses enzymes via post-translational linkage. Here, report split intein-mediated cascade wherein orthogonal inteins serve adapters for ligation. A genetically...