A5101524708- Cellular transport and secretion
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lipid Membrane Structure and Behavior
- Telomeres, Telomerase, and Senescence
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neuropharmacology Research
- Mesenchymal stem cell research
- Hemoglobinopathies and Related Disorders
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Digital Imaging for Blood Diseases
- AI in cancer detection
- Cellular Mechanics and Interactions
- Sarcoma Diagnosis and Treatment
- Copper Interconnects and Reliability
- Alzheimer's disease research and treatments
- Advanced Software Engineering Methodologies
- Genetics and Neurodevelopmental Disorders
- Hematopoietic Stem Cell Transplantation
- Congenital Anomalies and Fetal Surgery
- Electrodeposition and Electroless Coatings
- Autism Spectrum Disorder Research
- Prenatal Screening and Diagnostics
- Bacteriophages and microbial interactions
Seoul National University
2012-2022
Seoul National University Hospital
2018
The white blood cell differential count of the bone marrow provides information concerning distribution immature and mature cells within maturation stages. results such examinations are important for diagnosis various diseases follow-up care after chemotherapy. However, manual, labor-intensive methods to determine lead inter- intra-variations among obtained by hematologists. Therefore, an automated system conduct is highly desirable, but several difficulties hinder progress. There variations...
The prefibrillar form of soluble amyloid-β (sAβ1-42) impairs synaptic function and is associated with the early phase Alzheimer's disease (AD). We investigated how sAβ1-42 led to presynaptic defects using a quantum dot-based, single particle-tracking method monitor vesicle (SV) trafficking along axons. found that prevented new synapse formation induced by chemical long-term potentiation (cLTP). In cultured rat hippocampal neurons, nanomolar amounts impaired Ca2+ clearance from terminals...
The type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K) family members and their lipid product, 4,5-bisphosphate (PIP2) are important regulators of actin cytoskeleton. PIP5Kγ 90 kDa (PIP5Kγ90), an isoform PIP5K, localizes to focal adhesions (FAs) is activated via its interaction with the cytoskeletal protein, talin. Currently, regulatory signaling pathways talin–PIP5Kγ90 related FA dynamics cell motility not well understood. Considering presence Akt consensus motifs in PIP5Kγ90, we...
Despite being a highly enriched synaptic vesicle (SV) protein and candidate gene for autism, the physiological function of SCAMP5 remains mostly enigmatic. Here, using optical imaging electrophysiological experiments, we demonstrate that plays critical role in release site clearance at active zone. Truncation analysis revealed 2/3 loop domain directly interacts with adaptor 2, this interaction is its clearance. Knockdown (KD) exhibited pronounced depression accompanied by slower recovery SV...
Telomere erosion can lead to genomic instability and cancer progression. It has been suggested that the shortest telomere, not average telomere length (TL), is critical for cell viability. Some studies have shown shorter TL in myelodysplastic syndrome (MDS) patients but critically short telomeres, variability of within individual patient evaluated. Thus, we aimed investigate MDS assessed association with recurrent genetic mutations MDS. We measured bone marrow nucleated cells diagnostic...
Whether synaptic vesicles (SVs) are recovered via endosome-mediated pathways is a matter of debate; however, recent evidence suggests that clathrin-independent bulk endocytosis (CIE) endosomes functional and preferentially replenishes SV pools during strong stimulation. Here, using brefeldin-A (BFA) to block CIE, we found CIE retrieved minority SVs at developing CNS synapses stimulation, but its contribution increased up 61% mature synapses. Contrary previous views, BFA not only blocked...
The cytogenetic analysis of mesenchymal stromal cells (MSCs) is essential for verifying the safety and stability MSCs. An in situ technique, which uses grown on coverslips karyotyping minimizes cell manipulation, standard protocol chromosome amniotic fluids. Therefore, we applied technique MSCs compared quality metaphases results with classical G-banding chromosomal abnormalities fluorescence hybridization (FISH). Human adipose- umbilical cord-derived MSC lines (American Type Culture...
컴포넌트 기반 소프트웨어 설계는 개발된 컴포넌트의 조립을 농한 재사용으로 소프트웨어를 생성하는 것을 목표로 하고 있다. 이때 재사용 되는 컴포넌트들은 용도에 맞게 개조되어야 한다. 본 연구는 이러한 개조 방법을 트랜잭션 어댑터(TA : Transaction Adaptor)라는 컴포넌트를 이용하여 기존의 또는 레거시 시스템을 하는 것에 대한 연구이다. TA를 이용한 개조방법은 클라이언트와 호스트시스템 사이에 TA 사용하여, XML데이터를 스트림 형태로 변환하여 전송함으로서 또한 응용 프로그램의 개발에 XML 데이터에서 필요로 정보로 가공하는데 룰서버(RS ： Rule Server)를 이용함으로서 개발과 유지보수에 많은 효과를 얻을 수 있나. TA와 RS를 방법은 클라이언트 플랫폼이나 호스트의 종류에 관계없이 TA가 데이터 변환처리하고, RS가 데이터의 처리를 수행하여 할 The purpose of CBSD(Component Based Software Development) if...