A5005346506- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Macrophage Migration Inhibitory Factor
- Urinary Bladder and Prostate Research
- Nanoparticles: synthesis and applications
- Kidney Stones and Urolithiasis Treatments
- Building Energy and Comfort Optimization
- Epigenetics and DNA Methylation
- Chronic Myeloid Leukemia Treatments
- Advancements in Transdermal Drug Delivery
- Skin Protection and Aging
- Synthesis and Biological Evaluation
- Receptor Mechanisms and Signaling
- Graphene and Nanomaterials Applications
- Pelvic floor disorders treatments
- Blood disorders and treatments
- Youth Development and Social Support
- Aldose Reductase and Taurine
- Polydiacetylene-based materials and applications
- Protein Kinase Regulation and GTPase Signaling
- Cannabis and Cannabinoid Research
- RNA regulation and disease
- Nuclear Receptors and Signaling
- Conservation Techniques and Studies
- Biochemical effects in animals
Fundação Oswaldo Cruz
2025
National Human Genome Research Institute
2023-2025
Centro Hospitalar do Porto
2023-2024
Universidade do Vale do Rio dos Sinos
2023
Universidade do Porto
2023
Hospital da Senhora da Oliveira Guimarães
2023
Hospital Prof. Dr. Fernando Fonseca
2023
Hospital de Santo António
2023
Cardiff University
2019-2022
University of Aveiro
2017-2022
Small power is a substantial energy end-use in office buildings its own right, but also significantly contributes to internal heat gains. Technological advancements have allowed for higher efficiency computers, yet current working practices are demanding more out of digital equipment. Designers often rely on benchmarks inform predictions small consumption, demand and These date fail account the variability equipment speciation usage patterns different offices. This paper details two models...
The tailor-made character of deep eutectic solvents (DES) turns them very attractive to be used in several applications, including health-related areas such as pharmaceutical, nutraceutical, and cosmetic industries. However, although DES has been touted "green" solvents, works proved that their potential toxicity should not neglected. Using the premise applicability pharmaceutical sectors, we chose two cell lines work a skin model (keratinocytes HaCaT tumor melanocytes MNT-1), assess...
Nonenveloped viruses, such as hepatitis A virus (HAV) and parvovirus B19 (B19V), are not inactivated by detergents solvents commonly used to manufacture plasma derivatives. Cases of transfusion-transmitted HAV B19V have already been described in several countries. This study aimed determine the incidence asymptomatic infections blood donors from Rio de Janeiro evaluate residual risk transmission derivative recipients. From 2018 2019, 1,204 samples were tested PCR real-time PCR. genotypes...
Subversion of transcription factor (TF) activity in hematopoietic stem/progenitor cells (HSPCs) leads to the development therapy-resistant leukemic stem (LSCs) that drive fulminant acute myeloid leukemia (AML). Using a conditional mouse model where zinc-finger TF Gata2 was deleted specifically cells, we show knockout rapid and complete cell-autonomous loss adult cells. By using short hairpin RNAi target GATA2, also identify requirement for GATA2 human HSPCs. In Meis1a/Hoxa9-driven AML,...
Familial platelet disorder (FPD) is associated with germline RUNX1 mutations, establishing a preleukemic state and increasing the risk of developing leukemia. Currently, there are no intervention strategies to prevent leukemia progression. Single-cell RNA sequencing (n = 10) combined functional analysis samples from patients RUNX1-FPD > 75) revealed that FPD hematopoietic stem progenitor cells (HSPCs) displayed increased myeloid differentiation suppressed megakaryopoiesis because activation...
The involvement of cAMP- and Ca 2+ -mediated pathways in the activation tyrosine hydroxylase (TH) gene expression by nicotine was examined PC-12 cells. Extracellular elevations intracellular concentration ([Ca ] i ) were required for to increase TH mRNA. nicotine-elicited rapid rise [Ca inhibited blockers either L-type or N-type, a lesser extent P/Q-, but not T-type, voltage-gated channels. With continual treatment, returned basal levels within 3–4 min. After lag ∼5–10 min, there smaller...
RUNX3 is a transcription factor dysregulated in acute myeloid leukemia (AML). However, its role normal development and poorly understood. Here we investigate expression both settings the impact of dysregulation on myelopoiesis. We found that mRNA was stable during hematopoiesis but decreased with granulocytic differentiation. In AML, overexpressed many disease subtypes, downregulated AML core binding abnormalities, such as RUNX1::ETO. Overexpression human hematopoietic stem progenitor cells...
The protein kinase C (PKC) family of serine/threonine kinases are pleiotropic signaling regulators and implicated in hematopoietic development. Only one isoform however, PKCϵ, has oncogenic properties solid cancers where it is associated with poor outcomes. Here we show that PKCϵ significantly overexpressed acute myeloid leukemia (AML; 37% patients). In addition, expression AML was a significant reduction complete remission induction disease-free survival. Examination the functional...
Abstract: The relationship between elevations in intracellular free Ca 2+ concentration ([Ca ] i ) by different mechanisms and tyrosine hydroxylase (TH) gene expression was examined. Depolarization an elevated K + triggered rapid sustained increases [Ca from a basal level of ∼50 to 110–150 n M three‐ fourfold TH mRNA levels, requiring extracellular calcium but not inositol 1,4,5‐trisphosphate (IP 3 ). On the other hand, bradykinin or thapsigargin, both which induce release stores via IP...
Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, use green and faster methods that allow control their properties is prime importance. Thus, present study reports a one-minute microwave-assisted synthesis fucoidan-coated AuNPs controllable size high antitumoral activity. The NPs were synthesized using fucoidan-enriched fraction extracted from...
Background and objective Patients with neurogenic bladder (NB) are at a higher risk of developing chronic kidney disease (CKD). Due to their lower muscle mass, the estimated glomerular filtration rate (eGFR) based on creatinine (Cr) may be overestimated delay diagnosis renal failure. This study compared eGFR calculated different equations Cr and/or cystatin C (CysC) in children NB, differences between patients mass (underdeveloped limbs) those independent gait (less depletion). Methods We...
Graphene-related two-dimensional nanomaterials possess very technically promising characteristics, but gaps exist regarding their potential adverse health effects. Based on nano-thickness and lateral micron dimensions, nanoplates exhibit particular aerodynamic properties, including respirability. To develop a lung-focused, in vitro/in vivo screening approach for toxicological hazard assessment, various graphene-related nanoplates, i.e., single-layer graphene (SLG), nanoplatelets (GNP),...
RUNX proteins belong to a family of transcription factors essential for cellular proliferation, differentiation, and apoptosis with emerging data implicating RUNX3 in haematopoiesis haematological malignancies. Here we show that plays an important regulatory role normal human erythropoiesis. The impact altering expression on erythropoiesis was determined by transducing CD34+ cells overexpression or shRNA knockdown vectors. Analysis mRNA showed levels decreased during Functionally, had modest...
Adrenoleukodystrophy, a rare genetic disease associated with the X chromosome (X-ALD - X-linked adrenoleukodystrophy), predominantly affects males and stems from mutations in