Surface soil samples were collected near the Open Pit Bor (S1) and Cerovo (S2), a grassland along Borska Reka River (S3) an unpolluted garden Slatina village (reference site). Spontaneous plants (dandelion, nettle, coltsfoot, creeping buttercup) vegetables (onion, garlic, carrot, parsley, celery, potatoes, dill, sorrel) obtained from former three sites reference site, respectively. The analyzed for Zn, Cu, Fe, Mn, Pb via FAAS. Pollution indices indicated low-to-moderate contamination at S1,...

Herbicides are being used more and to increase productivity in agriculture, but their excessive has been shown lead adverse effects on the environment, especially soil. Within this study effect of herbicide S-metolachlor activity several enzymes (dehydrogenase, protease, phosphatase urease) found loamy-clayey soil analyzed. There were seven experimental variants corresponding untreated application six distinct doses samples maintained laboratory conditions. Biochemical analyzes have...

Triticonazole is a fungicide used to control diseases in numerous plants. The commercial product racemate containing (R)- and (S)-triticonazole its residues have been found vegetables, fruits, drinking water. This study considered the effects of triticonazole on soil microorganisms enzymes human health by taking into account enantiomeric structure when applicable. An experimental method was applied for assessing enzymes, stereoisomers were assessed using computational approach. There...

Land snails are highly tolerant to cadmium exposure and able accumulate soil independently of food ingestion. However, little information exists on the kinetics retention in terrestrial gastropods exposed an increase content, over time. There is also knowledge about how cadmium-polluted soils influences shell growth architecture. In this context, we examined accumulation hepatopancreas juvenile Cantareus aspersus elevating high levels soil. Also, toxicity was assessed using a range...

This study assesses the effects of herbicide oxyfluorfen on activities enzymes and populations soil microorganisms by considering experiments samples maintained in both laboratory field conditions. Furthermore, molecular docking approach was used to evaluate interactions with found soil. There a dose dependent inhibitory effect against dehydrogenase, phosphatase, protease urease. The obtained for under conditions usually reflected different trend than those conditions, emphasizing influence...

The aim of the present study was to formulate new variants synthetic and animal-source bone materials coated with different biopolymers, containing antibiotics (ampicillin oxacillin) alendronate drugs. Validation consists in establishing mixtures that do not interactions between components. These should act as a drug delivery system alongside base. Natural polymers such chitosan, alginate, kappa-carrageenan have been shown be optimal for due their intrinsic biocompatibility. Binary, ternary,...

The purpose of this study was to quantify the effect difenoconazole (DFC) on activity a few enzymes commonly found soil: dehydrogenase, urease, phosphatase and protease. Three experimental variants were established: in field conditions with variable temperature (10-21 ?C, A1-A3), under laboratory constant (30 B1-B3) untreated soil (C variant). commercial product "Score 250EC" 250 g DFC L-1 used at following concentrations: 0.037 mg g-1 (variants A1 B1), 0.075 A2 B2) 0.150 A3 B3). urease...

Sediment bacterial communities are key players in biogeochemical cycling of elements the aquatic environment. Copper mining, smelting, and processing operations located Bor area (Serbia) major environmental hot spots lower Danube Basin Western Balkans. In present study, we evaluate influence trace element (TE) concentration sediments physico-chemical properties water on sediment microbial streams adjacent to Smelter Complex (RTB Bor, Serbia). The degree which metabolic activities biota...

First-order sinking cave streams experience considerable hydrological variability, including spates and periods of base-flow during dry seasons. Early-summer flooding on a first-order stream in Ciur-Ponor Cave (Romania) represented suitable opportunity to test the response macroinvertebrate community basal food resources quantity diversity such disturbance event. The invertebrate (i.e., woody debris, leaves, fine particulate organic matter epilithon) were collected from three sampling sites,...

Ionic liquids (ILs) exhibit cytotoxic effects on prokaryotic and eukaryotic cells. In this study, the antibacterial antiproliferative activities of tetrahexylammonium bromide‑based ILs were investigated. order to evaluate therapeutic potential these ionic liquids, firstly microbiological assay using both Gram‑positive Gram‑negative bacteria conducted by employing Disk‑Diffusion 2,3,5‑triphenyltetrazolium chlorine (TTC) methods assess antimicrobial effects. Likewise, antitumor 2D 3D cell...

herbicides, have a broad spectrum effect on weeds, in relatively low doses and with much reduced toxicity livestock. In this study were used two herbicides: dacsulfuron the active substance chlorsulfuron (0.005 - 0.035 ?g/g soil) butoxone MCPB-Na mg/L/g soil). The samples collected from depth of 0-20 cm chernozem soil. herbicide was estimated by measuring activity catalase, actual potential dehydrogenase, urease cellulase activities. All being incubated for 10 days at 27?C using Sapp medium...

Abstract A molecular docking study was undertaken using the programs SwissDock and PatchDock to assess interactions of bacterial chitinases belonging GH18 GH19 families with two herbicides (chlorsulfuron nicosulfuron) fungicides (difenoconazole drazoxolon). Both predict that all considered pesticides bind active sites produced by soil microorganisms. There are correlations for predicted binding energy values receptor-ligand complexes obtained consolidating prediction chitinases-pesticides...

The indoor air quality in public buildings is essential for the health of employees and visitors. To investigate potential influence airborne germ loads on human health, two sampling campaigns were conducted during 2009 several Timisoara (Romania). revealed highly significant differences among different sites. Cluster analysis accurately classified investigated into three main groups most aerial microorganisms, measured values rarely fell below normal concentrations environments. Although...

The environmental impact of inorganic pollution is pronounced in water adjacent to Bor Copper Smelter Complex (RTB Bor, Serbia), with Cu, Zn, Pb, and As being the main determinants aquatic pattern. Communities microorganisms present sediments are mainly affected by heavy metal pollution. Some groups bacteria can be considered bio-indicators, due their sensibility ability bioaccumulate metals, thus contributing reducing This study investigates relationships between trace element accumulation...

Soil microorganisms play an important role in the decomposition of organic and inorganic matter soil, being capable breaking down different types xenobiotic substances soil. Application herbicides crops is commonly used agriculture to increase agricultural productivity weed destruction. Two sulfonylurea (tribenuron methyl nicosulfuron) were applied soil samples doses. The effect on enzymatic activities main groups involved nitrogen cycle (ammonifying, nitrifying denitrifying bacteria) was...

The present study quantified the effect of difenoconazole (DFC) on bacteria and mold communities grown in laboratory conditions, treated soil microcosms difenoconazole, using following concentrations: control, half dose (0.037 mg DFC / g soil, HD), normal (0.075 ND) double (0.150 DD). microbiological analysis included mesophilic involved nitrogen cycle: aerobic anaerobic nitrogen-fixing, ammonifying, nitrifying denitrifying bacteria. were incubated at 37�C identified qualitatively solid...

The interest in the antimicrobial actions of chitosan is due to its multiple properties and effects. aim study was assess potential antibacterial effects applied on 7 bacterial strains: Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Enterococcus faecalis, Clostridium perfringens, Legionella pneumophila Staphylococcus aureus. Six different concentrations were dissolved 1% acetic acid, following two working protocols (Kirby-Bauer method testing for cell viability)....

Acute and chronic exposure to lead induces oxidative damage morphologic physiological changes in tissues organs. The aims of this study were determine the concentration heavy metals like Zn, Al, Li, Cu Pb different organs identify histopathological liver kidneys laboratory animals case intoxication. In most cases, metal concentrations analyzed control group are significantly lower than values determined experimental groups. High caused increased kidney damage.

This study focusses on the investigation of potential binding fungicide difenoconazole to soil chitinases using a computational approach. Computational characterization substrate sites Serratia marcescens and Bacillus cereus Fpocket tool reflects role hydrophobic residues for high local density both sites. Molecular docking reveals that is able bind active sites, energies being comparable.