In this study, we conducted a noncarcinogenic risk assessment of consuming vegetables and fruits grown in two old mining areas from the Banat area Southern Carpathians (Romania), Moldova Veche (M) Rusca Montana (R) nonpolluted reference located near village Borlova (Ref). Concentrations Fe, Mn, Zn, Cu, Ni, Cd Pb soils commonly eaten were measured used for calculating weighted estimated daily intake metals (WEDIM), target hazard quotients (THQ) total (TTHQ) normal consumption adults. Levels...

Copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) can pose serious threats to environmental health because they tend bioaccumulate in terrestrial ecosystems. We investigated under field conditions the transfer of these heavy metals a soil-plant-snail food chain Banat area, Romania. The main goal this paper was assess Roman snail (Helix pomatia) usefulness monitoring as bioindicator metal accumulation. Eight sampling sites, selected by different history (HM) exposure, were chosen be sampled...

Background and Objectives: Sodium butyrate (NaB) is a potent modulator of cancer-related gene networks. However, its precise mechanisms action effects at elevated doses remain insufficiently explored. This study investigated the impact NaB physiologically relevant on key cellular metrics (viability, confluence, cell number, morphology, nuclear integrity) comprehensive set apoptosis proliferation regulators (including underexplored genes) in colorectal cancer (CRC) cells. Materials Methods:...

(1) Background: Hibernation in Lutrasil-covered enclosures led to elevated mortality rates for Cornu aspersum on Romanian farms. This two-year study evaluated the feasibility of adapting indoor hibernation technology Italian outdoor snail farming (IOSF) system as a solution overwintering mature C. snails. (2) Methods: Body weight, survival rates, and factors during key phases (purging overwintering) were monitored three commercial farms using different scenarios. Labor efficiency was...

Background and Objectives: Knee joint injuries incur substantial healthcare socioeconomic burdens worldwide. The connection between various demographic clinical factors meniscal injury patterns in patients undergoing surgery for rupture remains underexplored, especially Eastern European cohorts. This study aimed to determine the influence of age, sex, history previous on types knee adults aged 35 years older surgery. Materials Methods: A single-site exploratory retrospective analysis was...

Background and Objectives: episodes of acute decompensation in chronic heart failure (ADHF), a common health problem for the growing elderly population, pose significant socio-economic burden on public systems. Limited knowledge is available both endothelial function cardio-metabolic profile old adults hospitalized due to ADHF. This study aimed investigate connection between asymmetric dimethylarginine (ADMA)—a potent inhibitor nitric oxide—and key biomarkers this category high-risk...

5-methylcytosine (5mC) is a key epigenetic mark which influences gene expression and phenotype. In vertebrates, this sensitive to Cd exposure, but there no information linking such an event with changes in global 5mC levels terrestrial gastropods despite their importance as excellentecotoxicological bioindicators of metal contamination. Therefore, we first evaluated total content DNA the hepatopancreas adult Cantareus aspersus aim determine whether responsive exposure. The experiment was...

Land snails are highly tolerant to cadmium exposure and able accumulate soil independently of food ingestion. However, little information exists on the kinetics retention in terrestrial gastropods exposed an increase content, over time. There is also knowledge about how cadmium-polluted soils influences shell growth architecture. In this context, we examined accumulation hepatopancreas juvenile Cantareus aspersus elevating high levels soil. Also, toxicity was assessed using a range...

Little information exists on YKL-40-a key protein in tissue remodeling-and complete blood count (CBC) parameters during acute exacerbations of advanced chronic obstructive pulmonary disease (COPD). This pilot exploratory study (August 2020-January 2021) investigated the connection between serum YKL-40 levels and CBC profile sex- age-matched individuals with severe COPD (GOLD stage III, n = 23, median age 66 years, 65.21% males) very IV, 24, 66.5 74.81% males). The measured were YKL-40,...

Sediment bacterial communities are key players in biogeochemical cycling of elements the aquatic environment. Copper mining, smelting, and processing operations located Bor area (Serbia) major environmental hot spots lower Danube Basin Western Balkans. In present study, we evaluate influence trace element (TE) concentration sediments physico-chemical properties water on sediment microbial streams adjacent to Smelter Complex (RTB Bor, Serbia). The degree which metabolic activities biota...

An extensive literature exists regarding the cellular, physiological, and genetic effects of cadmium (Cd)-A highly toxic, but commonly used trace metal in modern industry. However, limited data are available on its epigenetic effects, especially for terrestrial sentinel invertebrates. We determined Cd retention, total DNA methylation, methylation status 5' end Cd-MT gene hepatopancreas brown garden snail, Cornu aspersum, fed sulfate four weeks. Bodyweight changes survival were also measured....

This is the first study investigating clinical relevance of 5-hydroxymethylcytosine (5hmC) in genomic DNA from white blood cells (WBC) context prostate cancer (PCa) and other pathologies. Using an enzyme-linked immunosorbent assay, we identified significantly different distributions patients with low elevated 5hmC content WBC across controls (PCa), atypical small acinar proliferation (ASAP), benign prostatic hyperplasia (BPH). The measured values were within normal range for most PCa...

Adult bone marrow mesenchymal stromal cells (BMSCs) can easily be differentiated into a variety of cells. In vivo transplantation BMSCs-differentiated has had limited success, suggesting that these may not fully compatible with the they are intended to replace in vivo. We investigated structural and functional features BMSCs-derived adipocytes as compared from adipose tissue, structure functionality lipid vesicles formed during BMSCs differentiation adipocytes. Gas chromatography-mass...

Manganese is one of the most abundant metal in natural environments and serves as an essential microelement for all living systems. However, enrichment soil with manganese resulting from industrial inputs may threaten terrestrial ecosystems. Several studies have demonstrated harmful effects exposure by cutaneous contact and/or ingestion to a wide range invertebrates. The link between land snails has never been made although these invertebrates routinely come upper horizons through contact,...

Background and Objectives: Bronchial asthma (BA) chronic obstructive pulmonary disease (COPD) are not only common respiratory conditions but also major causes of morbidity mortality worldwide. There is, however, a surprising lack blood-based biomarkers for separating between these disorders. The aim this study was to assess the practical relevance using serum YKL-40, single or combined, purpose. Materials Methods: Subjects included Romanian patients with BA (n = 24) COPD 27). fibrinogen,...

Dietary ingestion is the main route of exposure to hazardous contaminants in land animals. Cadmium, a high-profile toxic metal, affects living systems at different organismal levels, including major storage organs (liver, kidneys), key for species survival (gonads), and epigenetic networks regulating gene expression. 5-methylcytosine (5mC) most common best-characterized mark among modified nucleosides DNA. This important player methylation-driven expression impacted by cadmium sentinel...

The role of DNA methylation in mollusks is just beginning to be understood. This review synthesizes current knowledge on this potent molecular hallmark epigenetic control gastropods—the largest class and ubiquitous inhabitants diverse habitats. Their machinery shows a high degree conservation CG maintenance mechanisms, driven mainly by DNMT1 homologues, the presence MBD2 MBD2/3 proteins as readers. mosaic-like landscape occurs context primarily confined gene bodies housekeeping genes....