A5057181220- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Plant and animal studies
- Autism Spectrum Disorder Research
- Insect-Plant Interactions and Control
- Nuclear Receptors and Signaling
- Environmental DNA in Biodiversity Studies
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Ovarian function and disorders
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Forensic and Genetic Research
- Chromatin Remodeling and Cancer
- Adipose Tissue and Metabolism
- DNA and Nucleic Acid Chemistry
University of Nottingham
2013-2024
Loughborough University
2018
University of Washington
2003-2011
University of Tübingen
2010
University of Chicago
2010
University of Cambridge
2010
World Institute on Disability
2008
Guy's Hospital
2002-2004
King's College London
2004
University of Denver
2003
Obesity and type 2 diabetes arise from a set of complex gene-environment interactions. Explanations for the heritability these syndromes environmental contribution to disease susceptibility are addressed by "thrifty genotype" phenotype" hypotheses. Here, merits both models discussed elements them used synthesize epigenotype" hypothesis. I propose that: (1) metabolic thrift, capacity efficient acquisition, storage use energy, is an ancient, trait, (2) environmentally responsive gene network...
Epigenetic inheritance, the transmission of gene expression states from parent to daughter cells, often involves methylation DNA. In eukaryotes, cytosine is a frequent component epigenetic mechanisms. Failure transmit faithfully methylated or an unmethylated state can lead altered phenotypes in plants and animals. A central unresolved question epigenetics concerns mechanisms by which locus maintains, changes, its methylation. We developed “hairpin-bisulfite PCR” analyze these This method...
Bisulfite treatment can be used to ascertain the methylation states of individual cytosines in DNA. Ideally, bisulfite deaminates unmethylated uracils, and leaves 5-methylcytosines unchanged. Two types bisulfite-conversion error occur: inappropriate conversion 5-methylcytosine thymine, failure convert cytosine uracil. Conventional requires hours exposure low-molarity, low-temperature (‘LowMT’) and, sometimes, thermal denaturation. An alternate, high-molarity, high-temperature (‘HighMT’)...
The survival of a species depends on its capacity to adjust changing environmental conditions, and new stressors. Such new, anthropogenic stressors include the neonicotinoid class crop-protecting agents, which have been implicated in population declines pollinating insects, including honeybees (Apis mellifera). low-dose effects these compounds larval development physiological responses remained largely unknown. Over period 15 days, we provided syrup tainted with low levels (2 µg/L−1)...
The concept that postnatal health and development can be influenced by events occur in utero originated from epidemiological studies humans supported numerous mechanistic (including epigenetic) a variety of model species. Referred to as the ‘developmental origins disease’ or ‘DOHaD’ hypothesis, primary focus large-animal until quite recently had been biomedical. Attention has since turned towards traits commercial importance farm animals. Herein we review evidence prenatal risk factors,...
Abstract Culture expansion of primary cells evokes highly reproducible DNA methylation (DNAm) changes. We have identified CG dinucleotides (CpGs) that become continuously hyper- or hypomethylated during long-term culture mesenchymal stem (MSCs) and other cell types. Bisulfite barcoded amplicon sequencing (BBA-seq) demonstrated DNAm patterns neighboring CpGs more complex without evidence continuous pattern development association to oligoclonal subpopulations. Circularized chromatin...
Genomic methylation patterns of mammals can vary among individuals and are subject to dynamic changes during development. In order gain a better understanding this variation, we have analyzed cytosine within 200 bp region at the CpG island human FMR1 gene from leukocyte DNA. is normally methylated inactivation X chromosome in females it also inactivated upon expansion CGG repeats fragile-X syndrome. Patterns (epigenotypes) were determined by sequencing bisulfite-treated alleles normal males...
Journal Article Conservation of a maternal-specific methylation signal at the human IGF2R locus Get access Oskar W. Smrzka, Smrzka Search for other works by this author on: Oxford Academic PubMed Google Scholar Ingrid Faé, Faé 1Department Blood Group SerologyAKH, enna, Austria Reinhard Stöger, Stöger Robert Kurzbauer, Kurzbauer Gottfried Fischer, Fischer Traudi Henn, Henn Andreas Weith, Weith Denise P. Barlow * *To whom correspondence should be addressed Human Molecular Genetics, Volume 4,...
Leptin is a fat hormone regulating energy homeostasis. Here, it reported that the promoter and CpG island of autosomal gene (LEP) tissue-specific differentially methylated region (T-DMR) subject to dynamic methylation in human mouse vivo. Highly variable densities cytosine were detected by hairpin-bisulfite PCR among cells adipose tissue peripheral blood leukocytes. Intermediate low levels characterize majority LEP epialleles. Low-density epialleles are often at specific CG site within...
Genetic alterations in the alpha-synuclein (SNCA) gene have been implicated Parkinson Disease (PD), including point mutations, multiplications, and sequence variations within promoter. Such may be involved pathology through structural changes or overexpression of protein leading to aggregation, as well impaired expression. It is, therefore, importance specify parameters that regulate SNCA expression its normal mutated state. We studied alleles a lymphoblastoid cell line blood cells patient...
Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation composition two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled high resolution mass spectrometry was used establish compare lipidomes brain skeletal muscle from young (4-11 weeks old) middle (78 healthy mice. In mitochondria had reduced levels fatty acids, particularly...
Abstract PCR amplification of limited amounts DNA template carries an increased risk product redundancy and contamination. We use molecular barcoding to label each genomic with individual sequence tag prior amplification. In addition, we include ‘batch-stamps’ that effectively a sample ID analysis date. This highly sensitive method identifies redundant contaminant sequences serves as reliable for positive identification desired sequences; can therefore capture accurately the diversity in...
5-methylcytosine (5mC) is a key epigenetic mark which influences gene expression and phenotype. In vertebrates, this sensitive to Cd exposure, but there no information linking such an event with changes in global 5mC levels terrestrial gastropods despite their importance as excellentecotoxicological bioindicators of metal contamination. Therefore, we first evaluated total content DNA the hepatopancreas adult Cantareus aspersus aim determine whether responsive exposure. The experiment was...
Abstract Maximizing crop yields relies on the use of agrochemicals to control insect pests. One most widely used classes insecticides are neonicotinoids that interfere with signalling neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains Africanized honey bees, Apis mellifera, as adaptation altered...
The CHD family of proteins comprises ATP-dependent chromatin remodeling enzymes, which combine chromodomains, with SWI2/SNF2 ATPase/helicase motifs and DNA-binding capability. In the last few years, have drawn increased attention, because some them were found to form large multi-subunit complexes, involved in transcription-related events like gene activation, suppression, or histone modification. We previously described identification CHD6, a protein subfamily III. present study, we report...
5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of cytosine base in nuclear DNA mammalian brains. We measured levels by enzyme-linked immunosorbent assay from post-mortem cerebella individuals with Parkinson's disease age-matched controls. showed no significant differences between control sample sets. In contrast, median were almost twice as high (p < 0.001) both male female compared The distinct profile identified cerebellar patients...