A5088268744- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Malaria Research and Control
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Hepatitis Viruses Studies and Epidemiology
- Cancer Research and Treatments
- Computational Drug Discovery Methods
- Global Maternal and Child Health
- Graphene and Nanomaterials Applications
- Nerve injury and regeneration
- Telomeres, Telomerase, and Senescence
- Genetics, Bioinformatics, and Biomedical Research
- Biological Research and Disease Studies
- Parasites and Host Interactions
University of California, San Francisco
2017-2018
City of Hope
2015-2018
Beckman Research Institute
2015-2018
City Of Hope National Medical Center
2015
Significance Protozoal proteasome is a validated target for antimalarial drug development, but species selectivity of reported inhibitors suboptimal. Here we identify with improved malaria β5 subunit over each active human proteasomes. These compounds kill the parasite in stage its life cycle. They interact synergistically β2 inhibitor and artemisinin. Resistance to arose through point mutation nonproteolytic β6 subunit. The same made mutant strain more sensitive less fit withstand...
Jumonji domain-containing protein 3 (JMJD3/KDM6B) demethylates lysine 27 on histone H3 (H3K27me3), a repressive epigenetic mark controlling chromatin organization and cellular senescence. To better understand the functional consequences of JMJD3 its expression was investigated in brain tumor cells. Querying patient profile databases confirmed overexpression high-grade glioma. Immunochemical staining two glioma cell lines, U251 U87, indicated intrinsic differences levels that were reflected...
In a recent trial of intermittent preventive treatment in pregnancy (IPTp) Uganda, dihydroartemisinin-piperaquine (DP) was superior to sulfadoxine-pyrimethamine (SP) preventing maternal and placental malaria.We compared genotypes using sequencing, fluorescent microsphere, quantitative polymerase chain reaction assays at loci associated with drug resistance Plasmodium falciparum isolated from subjects receiving DP or SP.Considering aminoquinoline resistance, increased prevalences mutations...
Dihydroartemisinin-piperaquine (DHA-PQ) is under study for intermittent preventive treatment during pregnancy (IPTp), but it may accelerate selection drug resistance. Understanding the relationships between piperaquine concentration, prevention of parasitemia, and decreased sensitivity can inform control policies optimization DHA-PQ dosing. Piperaquine concentrations, measures Plasmodium falciparum genotypes associated with aminoquinoline in Africa (pfmdr1 86Y, pfcrt 76T) were obtained from...
Tumor-tropic neural stem cells (NSCs) can be engineered to localize gene therapies invasive brain tumors. However, like other cell-based therapies, survival of therapeutic NSCs after transplantation is currently suboptimal. One approach prolonging cell transiently overexpress an antiapoptotic protein within the prior transplantation. Here, we investigate utility and safety this using a clinically tested, v - myc immortalized, human NSC line contain suicide gene, cytosine deaminase (CD-NSCs)....
Controlling malaria in high transmission areas, such as much of sub-Saharan Africa, will require concerted efforts to slow the spread drug resistance and impede transmission. Understanding fitness costs associated with development resistance, particularly within context transmission, can help guide policy decisions accomplish these goals, constraints might lead decreased drug-resistant strains. To determine if Plasmodium falciparum resistance–mediating polymorphisms impact on at different...
<p>Table S2. Lists of genes defining IPA analyses "Cancer"- and "Inflammatory response"-related, those common to both.</p>
<p>Supplemental Figure Legends</p>
<p>Figure S1. Effect of JMJD3 on nuclear architecture.</p>
<p>Figure S2. (A) U251.JMJD3wt cells lack γH2AX foci. immunostained for foci (red) and counterstained with DAPI (blue). As a positive control formation, were irradiated (Cs137 source) 10 Gy γ-radiation examined after 24 hr. Note the absence of in untreated cells. Scale bar = µm. (B) Western blot U251.JMJD3 showing relative deficit non-irradiated cells.</p>
<p>Table S1. Ratios (U251.JMJD3wt vs. vector control) and p-values for 37,814 transcripts 20,661 genes (duplicate removed) affected by JMJD3 over-expression in U251 cells.</p>
<div>Abstract<p>Jumonji domain-containing protein 3 (JMJD3/KDM6B) demethylates lysine 27 on histone H3 (H3K27me3), a repressive epigenetic mark controlling chromatin organization and cellular senescence. To better understand the functional consequences of JMJD3 its expression was investigated in brain tumor cells. Querying patient profile databases confirmed overexpression high-grade glioma. Immunochemical staining two glioma cell lines, U251 U87, indicated intrinsic differences...
<div>Abstract<p>Jumonji domain-containing protein 3 (JMJD3/KDM6B) demethylates lysine 27 on histone H3 (H3K27me3), a repressive epigenetic mark controlling chromatin organization and cellular senescence. To better understand the functional consequences of JMJD3 its expression was investigated in brain tumor cells. Querying patient profile databases confirmed overexpression high-grade glioma. Immunochemical staining two glioma cell lines, U251 U87, indicated intrinsic differences...
<p>Figure S1. Effect of JMJD3 on nuclear architecture.</p>
<p>Table S1. Ratios (U251.JMJD3wt vs. vector control) and p-values for 37,814 transcripts 20,661 genes (duplicate removed) affected by JMJD3 over-expression in U251 cells.</p>
<p>Supplemental Figure Legends</p>
<p>Table S2. Lists of genes defining IPA analyses "Cancer"- and "Inflammatory response"-related, those common to both.</p>
<p>Figure S2. (A) U251.JMJD3wt cells lack γH2AX foci. immunostained for foci (red) and counterstained with DAPI (blue). As a positive control formation, were irradiated (Cs137 source) 10 Gy γ-radiation examined after 24 hr. Note the absence of in untreated cells. Scale bar = µm. (B) Western blot U251.JMJD3 showing relative deficit non-irradiated cells.</p>