A5077883472- Congenital Heart Disease Studies
- Congenital heart defects research
- Pulmonary Hypertension Research and Treatments
- Cardiovascular Function and Risk Factors
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
- Cardiac Valve Diseases and Treatments
- Cardiovascular and Diving-Related Complications
- Vascular anomalies and interventions
- Tissue Engineering and Regenerative Medicine
- Mechanical Circulatory Support Devices
- Cardiovascular Conditions and Treatments
- Cardiovascular Issues in Pregnancy
- Coronary Artery Anomalies
- Mitochondrial Function and Pathology
- Electrospun Nanofibers in Biomedical Applications
- Tracheal and airway disorders
- Cardiac Arrhythmias and Treatments
- Congenital Diaphragmatic Hernia Studies
- Health, Environment, Cognitive Aging
- Birth, Development, and Health
- Genomic variations and chromosomal abnormalities
- Gestational Diabetes Research and Management
- Anesthesia and Neurotoxicity Research
- Cardiovascular Health and Disease Prevention
Shanghai Jiao Tong University
2015-2025
Shanghai Children's Medical Center
2015-2025
Shanghai Children's Hospital
2023-2024
Kentucky Science Center
2019
John Wiley & Sons (United States)
2019
Triangle
2019
Indianapolis Zoo
2019
Guangdong Academy of Medical Sciences
2016
Guangdong General Hospital
2016
Society of Thoracic Surgeons
2015
Total anomalous pulmonary venous connection (TAPVC) is a rare form of congenital heart disease. This study describes current surgical treatment strategies and experiences in cohort patients from 2 cardiac centers Shanghai Guangdong China.This retrospective included 768 operated on between 2005 2014. Although most (n=690) underwent conventional repair, sutureless technique was used 10% (n=78) cases. A multilevel mixed-effects parametric survival model competing-risk analysis were to analyze...
Tetralogy of Fallot (TOF) is the most common cyanotic heart defect in neonates. While there compelling evidence genetic contribution to etiology TOF, noncoding variants development remains unexplored. Potentially damaging de novo (NC DNVs) were detected from 141 Chinese nonsyndromic TOF trios (CHN-TOF) and compared those Pediatric Cardiac Genomics Consortium (PCGC). Bioinformatic analyses on previously coding DNVs performed identify developmental pathways affected TOF. but not PCGC patients...
Blood oxygen saturation (SaO2) is one of the most important environmental factors in clinical heart protection. This study used human samples and induced pluripotent stem cell-cardiomyocytes (iPSC-CMs) to assess how SaO2 affects CM cell cycle activities. The results showed that there were significantly more markers moderate hypoxia group (SaO2: 75% 85%) than other 2 groups (SaO2 <75% or >85%). In iPSC-CMs 15% 10% (O2) treatment increased markers, whereas 5% rapid change O2 decreased markers....
Background: The renewal capacity of neonate human cardiomyocytes provides an opportunity to manipulate endogenous cardiogenic mechanisms for supplementing the loss caused by myocardial infarction or other cardiac diseases. GSK-3β inhibitors have been recently shown promote cardiomyocyte proliferation in rats and mice, thus may be ideal candidates inducing proliferation. Methods: Human were isolated from right atrial specimens obtained during routine surgery ventricle septal defect cultured...
To study the effect of angulation between left pulmonary artery (LPA) and main on haemodynamics.A 3D model patient-specific (PA) was reconstructed as an original model. Four models with descendent LPA equalled to 120°, 110°, 100° 90°, were by computer-aided design for virtual simulation flow under different surgical strategies. Computational fluid dynamics introduced calculate blood in five models. Streamlines, wall shear stress, energy loss distribution ratio calculated compared determine...
Perinatal reduction in cardiomyocyte cell cycle activity is well established animal models and humans. However, infants with congenital heart disease (CHD) unknown, may provide important information to improve treatment. Human right atrial specimens were obtained from during routine surgery repair ventricular septal defects. The divided into three groups: group A (age 1-3 months); B (age, 4-6 C 7-12 months). dramatic fall the number of Ki67-positive CHD cardiac myocytes occurred after...
Hypoxia has been suggested to be both beneficial and harmful the proliferation of cardiomyocytes. This controversy remains unresolved, underlying mechanism by which hypoxia exerts its effects unclear. We here hypothesize that cardiomyocyte developmental stage may play a role.The embryonic ventricular myocyte cell line H9C2, primary isolated fetal cardiomyocytes, neonatal cardiomyocytes were cultured with normal O2 (21% O2) or under hypoxic conditions (10% for 7 days, then harvested Western...
Background Current mammalian models for heart regeneration research are limited to neonatal apex amputation and myocardial infarction, both of which controversial. RNA seq has demonstrated a very set differentially expressed genes between sham operated hearts in infarction models. Here, we investigated rats whether pressure overload the right ventricle, common phenomenon children with congenital disease, could be used as better animal model studies when considering cardiomyocyte...
Tetralogy of Fallot (TOF) results in chronic progressive right ventricular (RV) pressure overload and shunt hypoxemia. We investigated the global changes proteome RV among infant patients with without TOF to gain an insight into early remodeling. One hundred thirty-six differentially expressed proteins were identified using label-free LC–ESI-MS/MS analysis. Western blot revealed that expression 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2) increased significantly patients;...
Background: Ischemia-reperfusion injury (I/R) strongly affects the prognosis of children with complicated congenital heart diseases (CHDs) who undergo long-term cardiac surgical processes. Recently, α2-adrenergic receptor agonist Dexmedetomidine (Dex) has been reported to protect cardiomyocytes (CMs) from I/R in cellular models and adult rodent models. However, whether how Dex may human CMs young remains largely unknown. Methods Results: Human ventricular tissue tetralogy Fallot (TOF)...
Background Current right ventricular (RV) volume overload (VO) is established in adult mice. There are no neonatal mouse VO models and how affects postnatal RV development largely unknown. Methods Results Neonatal was induced by the fistula between abdominal aorta inferior vena cava on day 7 confirmed ultrasound, echocardiography, hematoxylin eosin staining. The RNA-sequencing results showed that top 5 most enriched gene ontology terms normal were energy derivation oxidation of organic...
The most common cyanotic congenital heart disease (CHD) requiring management as a neonate is transposition of great arteries (TGA). Clinically, up to 50% TGA patients develop some form neurodevelopmental disability (NDD), thought have significant genetic component. A "ciliopathy" and links with laterality disorders been proposed. This first report whole genome sequencing in TGA, sought identify clinically relevant variants contributing heart, brain defects.Initial analyses on 100 focussed...
This study set out to design different types of total cavopulmonary connections (TCPC) with dual superior venae cavae (SVC), taking into account sites for anastomosis from pulmonary arteries (PAs), and compare haemodynamic features in these virtual operative designs. The geometries bilateral bidirectional Glenn (BBDG) connection inferior vena cava (IVC) connected extracardiac conduit were reconstructed three-dimensional configurations according the magnetic resonance images (MRIs) two...
Abstract The ostium secundum atrial septal defect (ASDII) is the most common type of congenital heart disease and characterized by a left to right shunting oxygenated blood caused incomplete closure septum secundum. We identified familial form isolated ASDII that affects four individuals in family five shows autosomal dominant inheritance. By whole genome sequencing, we discovered new mutation (c.*1784T > C) 3′-untranslated region (3′UTR) ACTC1 , which encodes predominant actin embryonic...
Abstract Objectives Pulmonary vein stenosis (PVS), one of the most challenging clinical problems in congenital heart disease, leads to secondary pulmonary arterial hypertension (PAH) and right ventricular (RV) hypertrophy. Due lack a rodent model, mechanisms underlying PVS its associated effects are largely unknown, treatments minimally successful. This study developed neonatal rat model with aim increasing our understanding developing possible for PVS. Methods was created at postnatal day 1...
Background Ventricular septal defects (VSDs) are the most common and simplest type of congenital heart diseases (CHDs). Animal studies have suggested that downregulation Yes-associated protein 1 (YAP1) during embryonic development causes VSD-associated CHDs. However, how YAP1 contributes to isolated VSD (iVSD) is unclear. Methods Results Twenty right atrial specimens were obtained from iVSD patients routine cardiac surgery we assessed expression in these specimens. For controls, six normal...
Tissue engineered vascular grafts (TEVGs) with diameters <6 mm have broad applications. However, maintaining graft post-implantation patency and function is still difficult to achieve of urgent necessity. In this study, we use an effective strategy make the large-pore electrospun polycaprolactone (PCL) cotton material (Cotton) utilized immunoreactive pre-cultivation prepare a small diameter autologous TEVG. The assessment via scanning electron microscopy revealed that Cotton has favorable...
Left ventricular (LV) volume overload (VO), commonly found in patients with chronic aortic regurgitation (AR), leads to a series of left pathological responses and eventually irreversible LV dysfunction. Recently, questions about the applicability guideline for optimal timing valvular surgery correct AR have been raised regard both adult pediatric patients. Understanding how VO regulates postnatal development may shed light on best surgical or drug intervention. Prepubertal was induced by...
Introduction:: Research regarding post-operative brain protection after deep hypothermic circulatory arrest (DHCA) has gained attracted significant attention. We previously demonstrated that hydrogen can significantly reverse DHCA-induced damage. Method:: In the current research, we have established DHCA model successfully using a modified four-vessel occlusion method and injected miR-29s compounds into hippocampal tissue of rats. Result:: were surprised to find increased expression in rat...