A5102006333- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Mast cells and histamine
- Traditional Chinese Medicine Analysis
- Smart Grid and Power Systems
- Plant-based Medicinal Research
- Circular RNAs in diseases
- Calcium signaling and nucleotide metabolism
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Geoscience and Mining Technology
- Allergic Rhinitis and Sensitization
- Power Systems and Renewable Energy
- Power Systems and Technologies
- Coal Properties and Utilization
- Geomechanics and Mining Engineering
- Receptor Mechanisms and Signaling
- Cancer Genomics and Diagnostics
- Helicobacter pylori-related gastroenterology studies
- Ion channel regulation and function
- Histone Deacetylase Inhibitors Research
- Biomarkers in Disease Mechanisms
- Advanced Statistical Process Monitoring
- Phytochemical Studies and Bioactivities
- Food Allergy and Anaphylaxis Research
North University of China
2024
Xi'an Jiaotong University
2018-2021
Wenzhou Medical University
2021
Jiangsu Province Hospital
2020
First Affiliated Hospital of Xi'an Jiaotong University
2020
Quanzhou Normal University
2020
Nanjing Medical University
2020
Yangzhou University
2020
The University of Texas Health Science Center at Houston
2006-2010
Tianjin University
2004-2006
Altering the number of surface receptors can rapidly modulate cellular responses to extracellular signals. Some receptors, like transferrin receptor (TfR), are constitutively internalized and recycled plasma membrane. Other epidermal growth factor (EGFR), after ligand binding then ultimately degraded in lysosome. Routing different destinations suggests that distinct molecular mechanisms may direct their movement. Here, we report endosome-associated protein hrs is a subunit complex containing...
Movement through the endocytic pathway occurs principally via a series of membrane fusion and fission reactions that allow sorting molecules to be recycled from those degraded. Endosome is dependent on SNARE proteins, although nature proteins involved their regulation has not been fully elucidated. We found endosome-associated hepatocyte responsive serum phosphoprotein (Hrs) inhibited homotypic early endosomes. A region Hrs predicted form coiled coil required for binding Q-SNARE, SNAP-25,...
Saposhnikoviae Radix, the dried root of divaricata, is commonly used in traditional Chinese anti-allergic preparations, like Bofutsusho-san and Yupingfeng granules. A high-expression Mas-related G protein-coupled receptor X2 cell membrane chromatography coupled online with high-performance liquid combined an ion trap time-of-flight multistage mass spectrometry system was established for screening identifying components Radix. The validated excellent specificity suitability using appropriate...
Although endocrine therapies targeting estrogen receptor α (ERα) are effective in managing ER positive (+) breast cancer, many patients have primary resistance or develop to therapies. In addition, ER+ cancer with PIK3CA activating mutations and 11q13-14 amplification poor survival unclear mechanism. We uncovered that higher expression of deubiquitinase USP35, located 11q14.1, was associated survival. Estrogen enhanced USP35 protein levels by downregulating USP35-targeting miRNA-140-3p...
Abstract Adverse drug reactions of Danshen injection mainly manifested as pseudoallergic reactions. In the present study, salvianolic acid A and a pair geometric isomers (isosalvianolic C C) were identified components in by high‐expression Mas‐related G protein coupled receptor X2 cell membrane chromatography online with high‐performance liquid electrospray ionization tandem mass spectrometry. Their activities evaluated vitro assay, which consistent retention times on column. Salvianolic C,...
Abstract Mas‐related G protein‐coupled receptor X2 was a mast cell–specific mediating anaphylactoid reactions by activating cells degranulation, and it also identified as target for modulating cell–mediated inflammatory diseases. The anti‐anaphylactoid drugs used clinically disturb the partial effect of mediators released cells. small molecule specific antagonists may provide therapeutic action diseases in early stage. In this study, high expression cell membrane chromatography coupled...
The endosome-associated protein Hrs inhibits the homotypic fusion of early endosomes. A helical region containing a Q-SNARE motif mediates this effect as well its endosomal membrane association via SNAP-25, an receptor for Hrs. formation SNARE complex by displacing VAMP-2 from complex, suggesting mechanism which endosome fusion. We examined regulation complexes to probe how may function negative regulator. show that although NSF dissociates VAMP-2.SNAP-25.syntaxin 13 it has no on...
The number of surface membrane proteins and their residence time on the plasma are critical determinants cellular responses to cues that can control plasticity, growth differentiation. After internalization, ultimate fate many is dependent whether they sorted for internalization into lumenal vesicles multivesicular bodies (MVBs), an obligate step prior lysosomal degradation. To help elucidate mechanisms underlying MVB sorting, we have developed a novel cell-free assay reconstitutes sorting...
Adverse drug reactions of traditional Chinese medicine injection mainly manifested as pseudo-allergic reactions. In the present study, ginsenoside Rd, Ro, and Rg3 were identified components in Shengmai by a high-expression Mas-related G protein-coupled receptor X2 cell membrane chromatography coupled online with high-performance liquid mass spectrometry. Their activities evaluated vitro vivo assay. The three compounds further found to induce reaction through X2. Therefore, we concluded that...
Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by gastrointestinal respiratory systems. Conventional diagnosis based on positive studies evaluation of parameters including IgE IgG1 levels, acute skin response anaphylactic shock reactions. We developed a cell membrane chromatographic (CMC) method human mast cells (HMC-1) for screening potential allergens infant formula powders (IFMP). HMC-1 membranes were extracted mixed...
To address the high complexity and low accuracy issues of traditional methods in mixed coal vitrinite identification, this paper proposes a method based on an improved DeepLabv3+ network. First, MobileNetV2 is used as backbone network to reduce number parameters. Second, atrous convolution layer with dilation rate 24 added ASPP (atrous spatial pyramid pooling) module further increase receptive field. Meanwhile, CBAM (convolutional block attention module) mechanism channel multiplier 8...
AP1000 reactor is the typical ``third-generation'' nuclear power plant in world at present. The primary system of was modeled using RELAP5/MOD 3.3 code, and transient thermal hydraulic characteristics were analyzed
Abstract Background: CircRNA and miRNA, as classes of non-coding RNA, have been found to play pivotal roles in sheep reproduction. There are many reports circRNA miRNA ovary uterus, but few oviduct. In this study, RNA-Seq was performed analyze the expression profile oviduct between follicular phase luteal FecB BB (MM) ++ (WW) sheep. Results: The result showed that 15 circRNAs were differentially expressed genotype (MF VS. ML), no (WF WL). 9 1 miRNAs identified MF ML, WF WL, respectively. GO...
In order to solve the problems of low production efficiency, poor quality blanks, high labour intensity and inability meet market demand with manual gypsum mould, a novel design mould be applied into automatic planks was developed for ceramic handicrafts. This inherits advantages tenon–mortise connection adopts six-piece combination. According spherical radial movement, new opens closes, mostly avoiding movement interference significantly improving service life. The technique ‘six-point...