A5044510771- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Diamond and Carbon-based Materials Research
- Ion-surface interactions and analysis
- Fullerene Chemistry and Applications
- Nanocluster Synthesis and Applications
- Electron and X-Ray Spectroscopy Techniques
- nanoparticles nucleation surface interactions
- Advanced Electron Microscopy Techniques and Applications
- Pharmacological Effects and Toxicity Studies
- Crystallization and Solubility Studies
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Cardiomyopathy and Myosin Studies
- Advanced Nanomaterials in Catalysis
- Cellular Mechanics and Interactions
- X-ray Diffraction in Crystallography
- Force Microscopy Techniques and Applications
- Enzyme Structure and Function
- Bioactive Natural Diterpenoids Research
- Liver physiology and pathology
- CAR-T cell therapy research
- Muscle Physiology and Disorders
- Metal and Thin Film Mechanics
Wuhan University of Science and Technology
2021-2025
Xuzhou Medical College
2025
Hokkaido University
2018-2021
Florida State University
2009-2017
Chongqing University
2017
The University of Texas at Austin
2017
Henan University
2016
Kyushu University
2014
Fudan University
1999-2012
Shanghai Municipal Center For Disease Control Prevention
2009
SPHIRE (SPARX for High-Resolution Electron Microscopy) is a novel open-source, user-friendly software suite the semi-automated processing of single particle electron cryo-microscopy (cryo-EM) data. The protocol presented here describes in detail how to obtain near-atomic resolution structure starting from cryo-EM micrograph movies by guiding users through all steps determination pipeline. These are controlled new graphical user interface and require minimum intervention. Using this protocol,...
This paper reports a computational method, the quantized elastic deformational model, that can reliably describe conformational flexibility of protein in absence amino acid sequence and atomic coordinates. The essence this method lies fact that, modeling functionally important changes such as domain movements, it is possible to abandon traditional concepts structure (bonds, angles, dihedrals, etc.) treat an object. shape mass distribution object are described by electron density maps, at...
The electronic structures of the perovskite oxides $\mathrm{La}M{\mathrm{O}}_{3}(M=\mathrm{C}\mathrm{r},\mathrm{}\mathrm{M}\mathrm{n},\mathrm{}\mathrm{F}\mathrm{e},\mathrm{}\mathrm{C}\mathrm{o},\mathrm{}\mathrm{and}\mathrm{}\mathrm{Ni})$ have been studied in local-spin density approximation $\mathrm{LSDA}+U$ method with on-site Coulomb interaction parameter U varying from 0.0 to 10.5 eV, and exchange J, 0.35 0.95 eV. It is found that splitting occupiedspin-up bands corresponding unoccupied...
Molecules that facilitate targeted protein degradation (TPD) offer great promise as novel therapeutics. The human hepatic lectin asialoglycoprotein receptor (ASGR) is selectively expressed on hepatocytes. We have previously engineered an anti-ASGR1 antibody-mutant RSPO2 (RSPO2RA) fusion (called SWEETS) to drive tissue-specific of ZNRF3/RNF43 E3 ubiquitin ligases, which achieved hepatocyte-specific enhanced Wnt signaling, proliferation, and restored liver function in mouse models,...
Abstract Owing to their outstanding performances significantly superior that of the low‐ and medium‐entropy counterparts, high‐entropy diborides attracted extensive attention. Nevertheless, rising configuration entropy rendered not only better but also greater formation difficulty due strengthened sluggish‐diffusion effect. Herein, (Hf 0.167 Zr Ti Ta Nb V )B 2 , as first reported six‐principal‐component IVB–VB transition‐metal diborides, was successfully synthesized by a microwave...
The first total synthesis of the architecturally complex hetisine-type heptacyclic C20 -diterpenoid alkaloids (±)-spirasine IV and XI is reported. A/F/G/C tetracyclic skeleton with challenging N-C6 C14-C20 linkages was efficiently constructed by an intramolecular azomethine-ylide-based 1,3-dipolar cycloaddition unusual regioselectivity. SmI2 -mediated free-radical addition to arene moiety without prior dearomatization a stereoselective aldol reaction further enabled rapid access hetisine...
Titanium-based composites hold great promise in versatile functional application fields, including supercapacitors. However, conventional subtractive methods for preparing complex-shaped titanium-based generally suffer from several significant shortcomings, low efficiency, strictly simple geometry, specific surface area, and poor electrochemical performance of the products. Herein, three-dimensional Ti/TiN nanotube arrays with hierarchically porous structures were prepared using additive...
The antigen specificity and long serum half-life of monoclonal antibodies have made them a critical part modern therapeutics. These properties been coopted in number synthetic formats, such as antibody-drug conjugates, bispecific antibodies, or Fc-fusion proteins to generate novel biologic drug modalities. Historically, these new therapies generated by covalently linking multiple molecular moieties through chemical genetic methods. This irreversible fusion different components means that the...
Herein, we report the first investigation into kinetics of cationic-ligand-exchange processes through reaction Au25(SCH2CH2Ph)18 with a cationic thiol HS(CH2)11N(CH3)3+, which resulted in different populations two thiolate ligands (SR0 and SR+), that is, Au25(SR0)18–x(SR+)x. The ligand-exchange process was monitored by electrospray ionization mass spectrometry, is discussed combination 1H NMR results; these techniques reveal process, from typical neutral-thiol-to-neutral-thiol ligand...
SET domain protein lysine methyltransferases (PKMT) are a structurally unique class of enzymes that catalyze the specific methylation residues in number different substrates. Especially histone-specific PKMTs have received widespread attention because their roles regulation epigenetic gene expression and development some cancers. Rubisco large subunit methyltransferase (RLSMT) is chloroplast-localized PKMT responsible for formation trimethyl-lysine-14 Rubisco, an essential photosynthetic...
Abstract The synthesis of high‐purity and high‐yield Au 25 clusters protected by the basic pyridyl ethanethiol (HSCH 2 CH Py, 4‐PyET 2‐PyET) is presented. Single‐crystal X‐ray diffraction [Au (4‐PyET) 18 ] − ⋅Na + has revealed a structure similar to that known for phenyl ethanethiolate analogue, but with pyridyl‐N coordination Na , more relaxed ligand shell, profoundly layered arrangement in solid state. Because pendant Py moiety, (PyET) are endowed unique (de)protonation equilibria, which...
In this paper, the deposition of ${\mathrm{C}}_{20}$ fullerenes on a diamond $(001)\ensuremath{-}(2\ifmmode\times\else\texttimes\fi{}1)$ surface and fabrication thin film at 100 K were investigated by molecular dynamics (MD) simulation using many-body Brenner bond order potential. First, we found that collision dynamic single fullerene was strongly dependent its impact energy. Within energy range 10--45 eV, chemisorbed retained free cage structure. This is consistent with experimental...