A5003169178- Nitric Oxide and Endothelin Effects
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiac Ischemia and Reperfusion
- Adipokines, Inflammation, and Metabolic Diseases
- Respiratory Support and Mechanisms
- Intensive Care Unit Cognitive Disorders
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Cardiomyopathy and Myosin Studies
- Renin-Angiotensin System Studies
- Electron Spin Resonance Studies
- Electrochemical sensors and biosensors
- Muscle Physiology and Disorders
- Solar Thermal and Photovoltaic Systems
- Ion Transport and Channel Regulation
- Liver physiology and pathology
- Caveolin-1 and cellular processes
- Cardiac Fibrosis and Remodeling
- Cardiac Arrest and Resuscitation
- Antioxidant Activity and Oxidative Stress
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
- Solar-Powered Water Purification Methods
University of Talca
2015-2024
Center for Discovery
2024
Boston University
2024
Universidad Santo Tomás
2024
Universidad Autónoma de Asunción
2023
Universidade Federal de Viçosa
2023
Nesta
2023
Escuela Colombiana de Ingenieria Julio Garavito
2019
Pontificia Universidad Católica de Chile
2000-2017
Royal Victoria Hospital
2017
Although interactions between superoxide ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{O}}_{2}^{{\cdot}-}\end{equation*}\end{document} ) and nitric oxide underlie many physiologic pathophysiologic processes, regulation of this crosstalk at the enzymatic level is poorly understood. Here, we demonstrate...
Altered Ca 2+ homeostasis is a salient feature of heart disease, where the calcium release channel ryanodine receptor (RyR) plays major role. Accumulating data support notion that neuronal nitric oxide synthase (NOS1) regulates cardiac RyR via S -nitrosylation. We tested hypothesis NOS1 deficiency impairs -nitrosylation, leading to altered homeostasis. Diastolic levels are elevated in −/− and NOS1/NOS3 but not NOS3 myocytes compared with wild-type (WT), suggesting diastolic leakage. Measured...
Although protein S -nitrosylation is increasingly recognized as mediating nitric oxide (NO) signaling, roles for denitrosylation in physiology remain unknown. Here, we show that -nitrosoglutathione reductase (GSNOR), an enzyme governs levels of by promoting denitrosylation, regulates both peripheral vascular tone and β-adrenergic agonist-stimulated cardiac contractility, previously ascribed exclusively to NO/cGMP. GSNOR-deficient mice exhibited reduced depressed inotropic responses were...
Liver fibrosis is a complex process characterized by the excessive accumulation of extracellular matrix (ECM) and an alteration in liver architecture, as result most types chronic diseases such cirrhosis, hepatocellular carcinoma (HCC) failure. Maresin-1 (MaR1) derivative ω-3 docosahexaenoic acid (DHA), which has been shown to have pro-resolutive anti-inflammatory effects. We tested hypothesis that application MaR1 could prevent development animal model hepatic damage. Sprague-Dawley rats...
Both cardiac myocytes and stem cells (CSCs) express the receptor of growth hormone releasing (GHRH), activation which improves injury responses after myocardial infarction (MI). Here we show that a GHRH-agonist (GHRH-A; JI-38) reverses ventricular remodeling enhances functional recovery in setting chronic MI. This response is mediated entirely by GHRH (GHRHR), as demonstrated use highly selective antagonist (MIA-602). One month MI, animals were randomly assigned to receive: placebo, GHRH-A...
Diabetic cardiomyopathy refers to the manifestations in heart as a result of altered glucose homeostasis, reflected fibrosis, cellular hypertrophy, increased oxidative stress, and apoptosis, leading ventricular dysfunction. Since physical exercise has been indicated cardioprotective, we tested hypothesis that high-intensity training could reverse cardiac maladaptations produced by diabetes. For this, diabetes was induced rats single dose alloxan. were randomly assigned sedentary group or...
Cardiovascular disease (CVD) is the leading cause of death worldwide. Its prevention emphasizes three aspects: not smoking, physical activity and a healthy diet. Recently, we screened antithrombotic selected group fruits vegetables. Among them, tomato showed an important effect. The aim this study was to evaluate characterize platelet antiaggregatory (Solanum lycopersicum L.). For this, obtained aqueous methanolic extracts evaluated effect pH (2 10) temperature (22, 60 100°C) on activity....
Maresin-1 (MaR1) is a specialized pro-resolving mediator, derived from omega-3 fatty acids, whose functions are to decrease the pro-inflammatory and oxidative mediators, also stimulate cell division. We investigated hepatoprotective actions of MaR1 in rat model liver ischemia-reperfusion (IR) injury. (4 ng/gr body weight) was administered prior ischemia (1 h) reperfusion (3 h), controls received isovolumetric vehicle solution. To analyze function, transaminases levels tissue architecture...
Although the combined use of hydralazine and isosorbide dinitrate confers important clinical benefits in patients with heart failure, underlying mechanism action is still controversial. We used two models nitroso-redox imbalance, neuronal NO synthase-deficient (NOS1(-/-)) mice spontaneously hypertensive failure rats, to test hypothesis that (HYD) alone or combination nitroglycerin (NTG) restores Ca(2+) cycling contractile performance controls superoxide production isolated cardiomyocytes....
Duchenne muscular dystrophy may affect cardiac muscle, producing a dystrophic cardiomyopathy in humans and the mdx mouse. We tested hypothesis that oxidative stress participates disrupting calcium handling contractility mouse with established cardiomyopathy. found increased expression (fivefold) of NADPH oxidase (NOX) 2 hearts compared wild type, along superoxide production. Next, we impact NOX2 inhibition on isolated cardiomyocytes. Contractility was decreased myocytes this restored toward...
Impaired leptin signalling in obesity is increasingly implicated cardiovascular pathophysiology. To explore mechanisms for activity the heart, we hypothesized that physiological participates maintaining cardiac beta-adrenergic regulation of excitation-contraction coupling. We studied 10-week-old (before development hypertrophy) leptin-deficient (ob/ob, n=12) and C57Bl/6 (wild-type (WT), n=15) mice at baseline after recombinant infusion (0.3 mg kg-1 day-1 28 days, n=6 each group)....
S-nitrosylation of several Ca2+ regulating proteins in response to β-adrenergic stimulation was recently described the heart; however specific nitric oxide synthase (NOS) isoform and signaling pathways responsible for this modification have not been elucidated. NOS-1 activity increases inotropism, therefore, we tested whether induces NOS-1-dependent total proteins, ryanodine receptor (RyR2), SERCA2 L-Type channel (LTCC). In isolated rat heart, isoproterenol (10 nM, 3-min) increased cardiac...
<b><i>Background/Aims:</i></b> Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of membrane-bound enzyme to Golgi cytosol. <b><i>Methods:</i></b> In isolated, perfused rat arterial mesenteric beds, we evaluated effect changes flow...
The study of adipose tissue has received considerable attention due to its importance not just in maintaining body energy homeostasis but also playing a role number other physiological processes. Beyond storing energy, is important endocrine, immunological, and neuromodulatory functions, secreting hormones that participate the regulation homeostasis. An imbalance these functions will generate structural functional changes tissue, favoring secretion deleterious adipocytokines induce...
Salt-gradient solar ponds (SGSPs) are water bodies that capture and accumulate large amounts of energy. The design an SGSP field has never been analyzed in terms studying the optimal number must be built to maximize useful energy can collected field, or most convenient way connect ponds. In this paper, we use constructal find configuration field. A steady-state thermal model was constructed estimate by each SGSP, then a complementary developed determine final temperature defined mass flow...