A5090420456- Plant Genetic and Mutation Studies
- Light effects on plants
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- GABA and Rice Research
- Agriculture and Biological Studies
- Mollusks and Parasites Studies
- Magnetic and Electromagnetic Effects
- Botany and Plant Ecology Studies
- Aquatic Invertebrate Ecology and Behavior
- Agricultural Productivity and Crop Improvement
- Prion Diseases and Protein Misfolding
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
Comenius University Bratislava
2023-2025
Institute of Cell Biology
2018-2024
Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences
2024
Slovak Academy of Sciences
2024
National Academy of Sciences of Ukraine
2018-2024
Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female tissues, we develop validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, explicitly quantifies intratumoral heterogeneity. Here show mFISHseq has 93% accuracy compared...
Chronic and acute irradiations have drastic effects on flowering stage that plays an important role in further seed development can determine yield. The expression of the key genes, AP1, CO, GI, FT, FLC, LFY, sensitive to irradiation repair gene RAD51 proliferation PCNA2 were studied wild-type Arabidopsis thaliana (Columbia ecotype) under chronic irradiations.Chronic was performed using radioactive isotope 137СsCl two total doses 3 cGy 17 cGy, with dose rate 10-7 cGy/s 6.8 10-6 cGy/s,...
Abstract On a retrospective cohort of 1,082 FFPE breast tumors, we demonstrated the analytical validity test using multiplexed RNA-FISH-guided laser capture microdissection (LCM) coupled with RNA-sequencing (mFISHseq), which showed 93% accuracy compared to immunohistochemistry. The combination these technologies makes strides in i) precisely assessing tumor heterogeneity, ii) obtaining pure samples LCM ensure accurate biomarker expression and multigene testing, iii) providing thorough...
Ionizing radiation is a harsh environmental factor that could induce plant senescence. We hypothesized radiation-related senescence remodels proteome, particularly by triggering the accumulation of prion-like proteins in tissues. The object this study, pea (
Aim. This study aimed to characterize the role of jasmonate signaling pathway in flowering genes response acute and chronic ionizing irradiation plants. Methods. We used wild-type Arabidopsis thaliana jasmonic defective jin mutant Col0 ecotype our experiments. The was provided by 137СsCl with a total dose 17 cGy rate 6.8×10-6 cGy/s. experiment performed on 21 days old plants at 5.0 stage (Boyes 2001) X-rays 5 Gy 89 length stems leaves measured post-irradiation period. molecular genetic...
ВПЛИВ НИЗЬКИХ ДОЗ РЕНТГЕНІВСЬКОГО ВИПРОМІНЮВАННЯ НА ГЕНИ ФОТОПЕРІОДИЧНОГО МЕХАНІЗМУ РЕГУЛЯЦІЇ ЦВІТІННЯ РОСЛИННаведено результати вивчення впливу низьких доз іонізуючого випромінювання на активність генів фотоперіодичного механізму детермінації цвітіння.Опромінення рослин рентгенівськими променями здійснювалося четвертому тижні вегетації (стадія стрілки 5,0 згідно з класифікацією Boyes, 2001) дозами 3, 6, 9 та 15 Гр енергією фотона 6 МеВ потужністю 89 сГр/с.Дослідження було проведено ключових...