A5027838732- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Fungal and yeast genetics research
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cellular transport and secretion
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Microbial Metabolic Engineering and Bioproduction
- Parkinson's Disease Mechanisms and Treatments
- Endoplasmic Reticulum Stress and Disease
- Biofuel production and bioconversion
- Signaling Pathways in Disease
University of Edinburgh
2023-2025
Wellcome Centre for Cell Biology
2023-2025
Comenius University Bratislava
2023
Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female tissues, we develop validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, explicitly quantifies intratumoral heterogeneity. Here show mFISHseq has 93% accuracy compared...
Abstract Intricate interactions between RNA-binding proteins (RBPs) and RNA play pivotal roles in cellular homeostasis, impacting a spectrum of biological processes vital for survival. UV crosslinking methods to study protein-RNA have been instrumental elucidating their but can be limited by degradation target during the process, low signal-to-noise ratios, non-specific interactions. Addressing these limitations, we describe reCRAC (reverse CRAC), novel adaptation CRAC (crosslinking analysis...
Abstract On a retrospective cohort of 1,082 FFPE breast tumors, we demonstrated the analytical validity test using multiplexed RNA-FISH-guided laser capture microdissection (LCM) coupled with RNA-sequencing (mFISHseq), which showed 93% accuracy compared to immunohistochemistry. The combination these technologies makes strides in i) precisely assessing tumor heterogeneity, ii) obtaining pure samples LCM ensure accurate biomarker expression and multigene testing, iii) providing thorough...
Multigene tests provide information that may guide the optimal treatment regimen for breast cancer (BCa) patients. However, assignment of an individual tumor to any subtype/prognostic risk group shows only moderate reproducibility depending on assay, composition, gene list and expression thresholds. This single-sample discordance impedes clinical use raises important questions about which is right test whether multiple are better than one. We used multiplexed RNA fluorescent in situ...
3069 Background: Breast cancer (BCa) is a heterogeneous disease requiring precise diagnostic tools to guide effective treatment strategies. Current assays, including various multigene often fail adequately address the complex biology of BCa subtypes. To these limitations and enhance understanding biology, we developed validated novel diagnostic, prognostic, predictive tool, called mFISHseq. Methods: Our approach, mFISHseq, integrates multiplexed fluorescent in situ hybridization (FISH) four...
ABSTRACT Adaptation to environmental change is essential in all organisms, with RNA-binding proteins (RBPs) playing critical roles rapid cellular responses. We analyzed the largely uncharacterized yeast RBP Pin4, and its involvement adaptation glucose depletion. A UV crosslinking technique identify protein-RNA interactions (reCRAC) revealed that conditions Pin4 selectively binds a specific motif 3’ UTRs of mRNAs involved glycolysis, amino acid, mitochondrial metabolism. Following withdrawal,...