A5055735060- Microbial bioremediation and biosurfactants
- Enzyme Structure and Function
- Enzyme-mediated dye degradation
- Amino Acid Enzymes and Metabolism
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Animal Virus Infections Studies
- Steroid Chemistry and Biochemistry
- Historical, Literary, and Cultural Studies
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Media, Religion, Digital Communication
- Bacteriophages and microbial interactions
- Pesticide and Herbicide Environmental Studies
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Environmental Chemistry and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Polyamine Metabolism and Applications
Bielefeld University
2018-2024
Flavin-dependent halogenases catalyse halogenation of aromatic compounds. In most cases, this reaction proceeds with high regioselectivity and requires only the presence FADH2, oxygen, halide salts. Since marine habitats contain concentrations halides, organisms populating oceans might be valuable sources yet undiscovered halogenases. A new Hidden-Markov-Model (HMM) based on PFAM tryptophan halogenase model was used for analysis metagenomes. Eleven metagenomes were screened leading to...
Regulation at the RNA level by RNA-binding proteins (RBPs) and microRNAs (miRNAs) is key to coordinating eukaryotic gene expression. In plants, importance of miRNAs highlighted severe developmental defects in mutants impaired miRNA biogenesis. MiRNAs are processed from long primary-microRNAs (pri-miRNAs) with internal stem-loop structures endonucleolytic cleavage. The highly structured stem-loops constitute basis for extensive regulation biogenesis through interaction RBPs. However,...
Flavin-dependent halogenases regioselectively introduce halide substituents into electron-rich substrates under mild reaction conditions. For the enzyme Xcc4156 from Xanthomonas campestris , structure of a complex with cofactor flavin adenine dinucleotide (FAD) and bromide ion would be particular interest as this exclusively brominates model in vitro . Apo crystals diffracted to 1.6 Å resolution. The revealed an open substrate-binding site lacking loop regions that close off active...
l ‐Amino acid oxidases (LAAOs) catalyze the oxidative deamination of ‐amino acids to α‐keto acids. Recombinant production LAAOs with broad substrate spectrum remains a formidable challenge. We previously achieved this for highly active and thermostable LAAO4 Hebeloma cylindrosporum ( Hc LAAO4). Here, we crystallized proteolytically truncated surface entropy reduction variant solved its structure in substrate‐free form complex diverse substrates. The ability support aliphatic portion...
InlB, a bacterial agonist of the human receptor tyrosine kinase MET, consists an N-terminal internalin domain, central B repeat and three C-terminal GW domains. In all previous structures full-length InlB or construct lacking domains (InlB 392 ), there was no interpretable electron density for repeat. Here, crystal in which is resolved are described. These first to reveal relative orientation domain A wild-type structure two T332E variant together contain five crystallographically...