The reconstitution of glucose oxidase on a flavin-adenine-dinucleotide (FAD)-functionalized single-walled carbon nanotube (SWCNT) associated with an Au electrode yields electrically contacted biocatalyst (see picture). efficiency the electrical contact is controlled by length SWCNT.

Recent advances in RNA research have posed new directives biology and chemistry to uncover the complex roles of ribonucleic acids cellular processes. Innovative techniques visualize native RNAs, particularly, short, low-abundance RNAs live cells, can dramatically impact current on biology. Herein, we report a novel method for real-time, microRNA imaging inside cells based programmable oligonucleotide probes, which self-assemble through Cascade Hybridization Reaction (CHR).

Metal-organic frameworks (MOFs) are a class of porous materials known for their large surface areas. Thus, over the past few decades development MOFs and applications has been major topic interest throughout scientific community. However, many current conventional syntheses lengthy solvothermal processes carried out at elevated temperatures. Herein, we developed rapid light-induced synthesis by harnessing plasmonic photothermal abilities bipyramidal gold nanoparticles (AuBPs). The generality...

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTLighting-Up the Dynamics of Telomerization and DNA Replication by CdSe−ZnS Quantum DotsFernando Patolsky, Ron Gill, Yossi Weizmann, Taleb Mokari, Uri Banin, Itamar WillnerView Author Information Institute Chemistry Farkas Center for Light Induced Processes, The Hebrew University Jerusalem, Jerusalem 91904, Israel Cite this: J. Am. Chem. Soc. 2003, 125, 46, 13918–13919Publication Date (Web):October 24, 2003Publication History Received29 April...

Highly sensitive: Analysis of viral DNA (M13 phage) is possible by using a DNA-based machine that fueled dNTPs and synthesizes DNAzyme reporter units. The DNAzymes mimic peroxidase lead to colorimetric or chemiluminescent readout the analyzed DNA.

An aptamer-based machine is used for the amplified detection of low-molecular-weight analyte, cocaine. The aptamer sequence recognizing cocaine, 1, blocked to an inactive structure through its hybridization with 1a. In presence 2, folded form cocaine-aptamer complex 3, while releasing nucleotide mixture, dNTPs, polymerase, and nicking enzyme Nt.BbvC I, a polymerization-nicking strand displacement initiated on cocaine−aptamer that acts as "track". displaced 4 hybridizes hairpin nucleic 5...

A crop of gold circles and lines: Psoralen-functionalized Au nanoparticles incorporated into DNA, Au-nanoparticle-functionalized poly-L-lysine, yield linear circular nanowires, respectively, on mica surfaces (see atomic force microscopy images).

A new concept for the amplified electrochemical detection of 7229-base viral DNA M13φ is developed. thiolated 27-base nucleic acid (1) assembled on an Au-electrode. Hybridization between sensing interface and followed by polymerase-induced replication analyte in presence dCTP, dGTP, dATP, ferrocene-tethered-dUTP (2). The generated redox-active replica mediates electron transfer enzyme glucose oxidase (GOx) electrode activates bioelectrocatalyzed oxidation glucose. provides a biocatalytic...

Circular DNA is used as a template for the amplified detection of M13 phage ssDNA by rolling circle amplification (RCA) process that synthesizes DNAzyme chains, thus enabling colorimetric or chemiluminescent analyte.

Much of the interest in noble metal nanoparticles is due to their plasmonic resonance responses and local field enhancement, both which can be tuned through size shape particles. However, properties suffer from loss monodispersity that frequently associated with various morphologies nanoparticles. Here we show a method generate diverse monodisperse anisotropic gold nanoparticle shapes tip geometries as well highly tunable augmentations either oxidative etching or seed-mediated growth...

As crucial pieces in the puzzle of cancer and human aging, telomeres telomerase are indispensable modern biology. Here we describe a novel exponential isothermal amplification telomere repeat (EXPIATR) assay—a sensitive, simple, reliable vitro method for measuring activity cell extracts. Through strategically designed path nucleic acid amplifications, EXPIATR abandons expensive thermal cycling protocol achieves ultrafast detection: equivalent to single HeLa can be detected ∼25 min.

We report the construction of periodic DNA nanoribbons (DNRs) by a modified origami method. Unlike conventional origami, DNR scaffold is long, single-stranded tandem repeats, originating from rolling circular amplification (RCA). Consequently, number folding staple strands tremendously decreases hundreds to few, which makes production scalable and cost-effective, thus potentially removing barrier for practical applications nanostructures. Moreover, co-replicational synthesis RCA-based...

Nucleic acid amplification techniques have been among the most powerful tools for biological and biomedical research, vast majority of bioassays rely on thermocycling that uses time-consuming expensive Peltier-block heating. Here, we introduce a plasmonic photothermal method quantitative real-time PCR, using gold bipyramids light to achieve ultrafast thermocycling. Moreover, successfully extend our system other assays, such as isothermal nucleic restriction enzyme digestion.

Abstract Chemiresistive sensor arrays for cyclohexanone and nitromethane are fabricated using single‐walled carbon nanotubes (SWCNTs) that covalently functionalized with urea, thiourea, squaramide containing selector units. Based on initial sensing results 1 H NMR binding studies, the most promising selectors chosen further optimized. These optimized attached to SWCNTs simultaneously tested in a array. The sensors show very high level of reproducibility between measurements same across...

Programmed self-assembly of nucleic acids is a powerful approach for nano-constructions. The assembled nanostructures have been explored various applications. However, acid assembly often requires chemical or in vitro enzymatical synthesis DNA RNA, which not cost-effective production method on large scale. In addition, the difficulty cellular delivery limits vivo Herein we report strategy that mimics protein production. Gene-encoded duplexes are transcribed into single-stranded RNAs,...

A novel amplification route for DNA detection based on the deposition of gold a 10 nm Au-colloid/avidin conjugate label acting as 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce catalyzed piezoelectric crystals. Three different schemes described: (i) analysis 27-base nucleic acid fragment; (ii) entire M13ϕ (7229 bases); and (iii) single-base mismatch in DNA. Ultrasensitive accomplished by gold, limit ∼1 × 10−15 M.

DNA strands consisting of programmed sequence-specific domains were synthesized by the rolling circle amplification (RCA) process. The spatial positioning glucose oxidase (GOx) and horseradish peroxidase (HRP) on RCA-synthesized template via hybridization enabled activation bienzyme cascade. GOx-catalyzed oxidation yielded gluconic acid H2O2, resulting H2O2 oxidized 2,2′-azino-bis[3-ethylbenzthiazoline-6-sulfonic-acid] (ABTS2−) in presence HRP. enzyme cascade could not be activated absence...

The biomachinery of rolling-circle amplification relying on protein–aptamer interactions DNA scaffolds was used to create one-dimensional DNA–protein/DNA–protein–nanoparticle hierarchical assemblies. These conjugates can serve as further templates for the immobilization gold nanoparticles.

A unique DNA scaffold was prepared for the one-step self-assembly of hierarchical nanostructures onto which multiple proteins or nanoparticles are positioned on a single template with precise relative spatial orientation. The architecture is topologically complex ladder-shaped polycatenane in "rungs" ladder used to bring together individual rings mechanically interlocked structure, and "rails" available assembly, whose effectiveness has been demonstrated proteins, complementary DNA, gold...