A5088302363- Gut microbiota and health
- Colorectal Cancer Screening and Detection
- Infective Endocarditis Diagnosis and Management
- Gastric Cancer Management and Outcomes
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Oral microbiology and periodontitis research
- Enhanced Recovery After Surgery
- Cancer-related molecular mechanisms research
- Urinary Tract Infections Management
- Infant Nutrition and Health
- Central Venous Catheters and Hemodialysis
- Immune Cell Function and Interaction
- Forensic Anthropology and Bioarchaeology Studies
- Digestive system and related health
- Prenatal Screening and Diagnostics
- MicroRNA in disease regulation
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Immune cells in cancer
- Chemokine receptors and signaling
- Circular RNAs in diseases
- Actinomycetales infections and treatment
- Colorectal Cancer Surgical Treatments
- Gastrointestinal motility and disorders
Masaryk University
2019-2025
RECETOX
2019-2021
Masaryk Memorial Cancer Institute
2021
Abstract Many studies correlate changes in human gut microbiome with the onset of various diseases, mostly by 16S rRNA gene sequencing. Setting up optimal sampling and DNA isolation procedures is crucial for robustness reproducibility results. We performed a systematic comparison several kits, quantified their effect on bacterial gDNA quality composition estimates at all taxonomic levels. Sixteen volunteers tested three kits. All samples were consequently processed two found that choice both...
Early detection of colorectal cancer (CRC) significantly improves its management and patients' survival. Circular RNAs (circRNAs) are peculiar covalently closed transcripts involved in gene expression modulation whose dysregulation has been extensively reported CRC cells. However, little is known about their alterations the early phases carcinogenesis. In this study, we performed an integrative analysis circRNA profiles RNA-sequencing (RNA-Seq) data 96 cancers, 27 adenomas, matched adjacent...
Urinary or ureteral catheter insertion remains one of the most common urological procedures, yet is considered a predisposing factor for urinary tract infection. Diverse bacterial consortia adhere to foreign body surfaces and create various difficult treat biofilm structures. We analyzed 347 catheter- stent-related samples, treated with sonication, using both routine culture broad-range 16S rDNA PCR followed by Denaturing Gradient Gel Electrophoresis Sanger sequencing (PCR-DGGE-S). In 29...
Long-term dysbiosis of the gut microbiome has a significant impact on colorectal cancer (CRC) progression and explains part observed heterogeneity disease. Even though shifts in normal-adenoma-carcinoma sequence were described, landscape within CRC its associations with clinical variables remain under-explored. We performed 16S rRNA gene sequencing paired tumour tissue, adjacent visually normal mucosa stool swabs 178 patients stage 0-IV to describe association variables. identified new...
Background: Myeloid-derived suppressor cells (MDSCs) contribute to tumor escape from host immune surveillance and progression by producing tumor-promoting factors.We focused on clinical analytical MDSCs-related issues as potential bio markers regulators involved in progression.Patients Methods: We analyzed 10 patients with advanced colorectal carcinoma (CRC) (M1 subgroup) or without (M0 distant metastases at dia gnosis.Peripheral blood was collected gnosis prior treatment subsequently 12...
Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases conditions, such as prostate or bladder cancer, chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral urinary catheter samples from 128 patients by Polymerase Chain Reaction followed Denaturing Gradient Gel Electrophoresis Sequencing (PCR-DGGE-S), culture, 29 of these also 16S rRNA Illumina sequencing, establish A. schaalii’s prevalence in tract-related samples,...
<title>Abstract</title> Background Previous studies described the impact of various early life events on developing gut environment infants. Nevertheless, a comprehensive understanding time frame response healthy microbiome to specific factors, such as vaccination, introduction solid foods etc., and duration these changes is currently lacking. Hence, determining optimal sampling frequency effectively monitor complex development remains challenge. Methods To address gaps, we monitored...
Abstract Background Long-term dysbiosis of the gut microbiome has a significant impact on development, progression and aggressiveness colorectal cancer (CRC) may explain part observed heterogeneity disease from phenotypic, prognostic response to treatment perspectives. Although shifts in normal-adenoma-carcinoma sequence have been described, landscape within CRC its associations with clinical variables remain under-explored. Results We performed 16S rRNA gene sequencing paired tumour tissue,...