A5089079849- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- DNA and Nucleic Acid Chemistry
- Advanced Nanomaterials in Catalysis
- Biosensors and Analytical Detection
- Trace Elements in Health
- Graphene and Nanomaterials Applications
- Moringa oleifera research and applications
- Nanoparticle-Based Drug Delivery
- Selenium in Biological Systems
- Carbon and Quantum Dots Applications
- Metal complexes synthesis and properties
- Quantum Dots Synthesis And Properties
- Electrochemical Analysis and Applications
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Bee Products Chemical Analysis
- Crystallization and Solubility Studies
- Microfluidic and Capillary Electrophoresis Applications
- Polyamine Metabolism and Applications
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Nanocluster Synthesis and Applications
- X-ray Diffraction in Crystallography
- Bone Tissue Engineering Materials
Mendel University in Brno
2014-2024
Central European Institute of Technology
2015-2022
Brno University of Technology
2015-2022
Masaryk University
2019
The emergence of antibiotic resistance in pathogenic bacteria has become a global threat, encouraging the adoption efficient and effective alternatives to conventional antibiotics promoting their use as replacements. Titanium dioxide nanoparticles (TiO
Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex compounds. The aim this study was to observe the PtNPs in vitro amplification DNA fragment phage λ, on bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In synthesized were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry atomic absorption...
A highly porous scaffold is a desirable outcome in the field of tissue engineering. The structure mediates water-retaining properties that ensure good nutrient transportation as well creates suitable environment for cells. In this study, antibacterial collagenous scaffolds containing chitosan and selenium nanoparticles (SeNPs) agents were studied. addition increased application potential material infected chronic wounds. morphology, swelling, biodegradation, activity collagen-based...
Abstract Many studies correlate changes in human gut microbiome with the onset of various diseases, mostly by 16S rRNA gene sequencing. Setting up optimal sampling and DNA isolation procedures is crucial for robustness reproducibility results. We performed a systematic comparison several kits, quantified their effect on bacterial gDNA quality composition estimates at all taxonomic levels. Sixteen volunteers tested three kits. All samples were consequently processed two found that choice both...
Nanocomposite films that were based on furcellaran (FUR) and nanofillers (carbon quantum dots (CQDs), maghemite nanoparticles (MAN), graphene oxide (GO)) obtained by the casting method. The microstructure, as well structural, physical, mechanical, antimicrobial, antioxidant properties of was investigated. incorporation MAN GO remarkably increased tensile strength films. However, water content, solubility, elongation at break significantly reduced addition nanofillers. Moreover, containing...
The high doses of zinc oxide (ZnO) administered orally to piglets for the prevention diarrhea and increase growth rate can contaminate pig farms surrounding environment. Therefore, there is a need find replacement dietary ZnO with an equally effective alternative. In present study, effect two formulations phosphate-based nanoparticles (ZnA ZnC NPs) on performance, intestinal microbiota, antioxidant status, liver morphology was evaluated. A total 100 weaned were randomly divided into 10 equal...
Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent promising alternative antibiotics may lower antibiotic use consequently spread resistance traits among bacteria, including pathogens.Four formulations zinc (ZnA, ZnB, ZnC, ZnD) based on phosphates with...
Abstract In a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on nanoparticle surface influence both nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge biocompatibility risk exposure to nanomaterials is limited. Here, in vitro ex ovo naturally based crosslinked freeze-dried 3D porous collagen/chitosan scaffolds, modified thermostable fibroblast growth factor 2 (FGF2-STAB ® ), enhance healing...
Abstract Background Bovine mastitis is one of the main causes reduced production in dairy cows. The infection mammary gland mainly caused by bacterium Staphylococcus aureus, whose resistant strains make treatment with conventional antibiotics very difficult and result high losses. Therefore, it important to develop novel therapeutic agents overcome resistance mastitis-causing strains. In this study, selenium-tellurium based nanoparticles (SeTeNPs) were synthesized characterized. Their...
Enhanced antibacterial properties of nanomaterials such as TiO2 nanotubes (TNTs) and silver nanoparticles (AgNPs) have attracted much attention in biomedicine industry. The depend, among others, on the functionalization layer nanoparticles. However, more complex information about influence different layers nanoparticle decorated surfaces is still missing. Here we show array ∼50 nm diameter TNTs with AgNPs having polyvinylpyrrolidone, branched polyethyleneimine, citrate, lipoic acid,...
A voltammetric detection of doxorubicin and encapsulated in apoferritin structure at a carbon paste electrode is the main aim this study. The samples were measured by differential pulse voltammetry phosphate buffer (pH 5.5). Complex apoferritin-doxorubicin can be formed "opening"/"closing" due to changing pH value. value electrolyte was decreased sample done adding hydrochloric acid. We optimized experimental conditions as time accumulation deposition potential obtain limit for 1.0 μg/ml doxorubicin.
Using of platinum based cytostatic drugs in tumour diseases treatment results the need to detect these biological samples and determine study Pt-DNA adducts, which is highly important for investigation cisplatin-resistance developed during anti-tumour treatment. The aim this was not only electrochemical detection cisplatin with use formazone method but also preparation platinum-DNA complex content DNA. Primarily, we optimized manual using differential pulse voltammetry (DPV) PtCl2 cisplatin....
Rapid and sensitive detection is a key step in the effective early response to global hazard of various viral diseases. In this study, an integrated isolation hepatitis B virus (HBV)-specific DNA fragment by magnetic nanoparticles (MNPs) its immediate analysis microchip CGE was performed. Microfluidic CE chip used accommodate complete process MNPs including hybridization thermal denaturation followed separation. Beforehand, calibration curves HBV fragments were constructed. For MNPs,...
Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one the most serious health complications during a medical treatment. Therefore, this study is focused on isolation, characterization, and identification microorganisms prevalent in superficial wounds patients (n=50) presenting bacterial infection.After successful cultivation, bacteria were processed analyzed. Initially strains was performed through matrix-assisted laser...
Transformation of nutrients to their nano-form, such as selenium (Se) engineered nanonutrients (Se-ENNs), is expected enhance the absorption into fish and increase efficiency feed.