A5078507945- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- DNA and Nucleic Acid Chemistry
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Trace Elements in Health
- Nanoparticle-Based Drug Delivery
- Quantum Dots Synthesis And Properties
- Carbon and Quantum Dots Applications
- SARS-CoV-2 detection and testing
- Heavy Metal Exposure and Toxicity
- RNA Interference and Gene Delivery
- Metal complexes synthesis and properties
- Nanoparticles: synthesis and applications
- Carbon Nanotubes in Composites
- Advanced Chemical Sensor Technologies
- Graphene research and applications
- Nanopore and Nanochannel Transport Studies
- Bacterial biofilms and quorum sensing
- Pharmacological Effects and Assays
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
Mendel University in Brno
2014-2024
Institut Català de Nanociència i Nanotecnologia
2020
Central European Institute of Technology
2013-2019
Brno University of Technology
2013-2019
European Union
2015-2016
Charles University
2015
Interactions of silver phosphate nanoparticles (SPNPs) and selenium (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular protein level. Significant antibacterial effects both SPNPs SeNPs on S. were observed. At a concentration 300 μM, caused 37.5% inhibition bacterial growth totally inhibited growth. As these might performed due to interactions DNA proteins, interaction or amplified zntR gene was studied. The presence decreased melting temperatures...
Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex compounds. The aim this study was to observe the PtNPs in vitro amplification DNA fragment phage λ, on bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In synthesized were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry atomic absorption...
In this study, carbon quantum dots were used for enhancement of the electrochemical signals etoposide.
Abstract This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The integrates the extraction purification of nucleic acids, followed by amplification via either reverse transcription-quantitative polymerase chain reaction (RT–qPCR) or transcription loop-mediated isothermal (RT–LAMP). By meeting diverse diagnostic reagent needs, platform yields testing results that closely align with those commercial RT-LAMP RT‒qPCR systems. Notable advantages...
There is an arising and concerning issue in the field of bacterial resistance, which confirmed by number deaths associated with drug-resistant infections. The aim this study was to compare effects antibiotics on Staphylococcus aureus non-resistant strain strains resistant cadmium or lead ions. Metal were created gradual addition 2 mM solution metal ions (cadmium lead) S. culture. An increasing antimicrobial effect ampicillin, streptomycin, penicillin tetracycline (0, 10, 25, 50, 75, 150, 225...
In this study a device for automatic electrochemical analysis was designed. A three electrodes detection system attached to positioning device, which enabled us move the electrode from one well another of microtitre plate. Disposable carbon tip were used Cd(II), Cu(II) and Pb(II) ion quantification, while Zn(II) did not give signal in configuration. order detect all mentioned heavy metals simultaneously, thin-film mercury (TFME) fabricated by electrodeposition on surface tips. comparison...
Increasing urbanization and industrialization lead to the release of metals into biosphere, which has become a serious issue for public health. In this paper, direct electrochemical reduction zinc ions is studied using electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE). The (GO) was fabricated Hummers method on surface GCE by performing cyclic voltammograms from 0 −1.5 V. modification optimized properties electrodes were determined impedance spectroscopy...
In this study, the enhancement of electrochemical signals Cr(iii) and Cr(vi) by using an activated glassy carbon electrode (GCE) measured differential pulse voltammetry (DPV) is demonstrated. The GCE exhibited higher sensitivity for detection compared with bare GCE. By GCE, limit decreased 15.3 times (from 0.230 to 0.015 μM) in case determination 75 9.000 0.120 determination. Moreover, a simultaneous speciation analysis was presented. Scanning electron microscopy, elemental mapping,...
Doxorubicin is a commonly used antineoplastic agent in the treatment of many types cancer. Little known about interactions doxorubicin with cardiac biomolecules. Serious cardiotoxicity including dilated cardiomyopathy often resulting fatal congestive heart failure may occur as consequence chemotherapy doxorubicin. The purpose this study was to determine effect exposure on changes major amino acids tissue muscle (proline, taurine, glutamic acid, arginine, aspartic leucine, glycine, valine,...
Remote‐controlled robotic systems are being used for analysis of various types analytes in hostile environment including those called extraterrestrial. The aim our study was to develop a remote‐controlled platform (ORPHEUS‐HOPE) bacterial detection. For the ORPHEUS‐HOPE 3D printed flow chip designed and created with culture chamber volume 600 μL. rate optimized 500 μL/min. tested primarily detection 1‐naphthol by differential pulse voltammetry limit (S/N = 3) as 20 nM. Further, way how...
The introduced work represents an implementation of the automatic benchtop electrochemical station (BES) as effective tool for possibilities high-throughput preparation modified sensor/biosensors, speeding up development analytical method, and automation procedure determination paracetamol (PAR) dopamine (DOP) target analytes. Within gold nanoparticles screen-printed carbon electrode (AuNPs-SPCE) by electrodeposition, deposition potential EDEP, time tDEP, concentration HAuCl4 were optimized...
In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl2, Zn(his)Cl2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy use) instrumentation variable setting flows. Reduction signals under strictly defined controlled conditions (pH, temperature, flow rate, accumulation time applied potential) were studied. We showed that increasing concentration led a decrease in signal significant shift potential...
Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice targeted anchoring liposomes (with and without cholesterol) with encapsulated anticancer drugs antisense N-myc gene oligonucleotide attached its surface. To meet main aim, doxorubicin, ellipticine etoposide were prepared. They further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency estimated be 16%. hybridization process...
The aim of our study was to equip a remote-controlled robotic platform (ORPHEUS-HOPE) with electrochemical system for fast detection heavy metal ions in water. contains two cameras – one pan/tilt color camera both manual and automatic parameter settings, rigid high resolution precise vision zooms screen printed electrodes at the end manipulator. maximum payload manipulator is 0.85 kg. Screen consist working electrode (WE), reference (RE) auxiliary (AE).WE (carbon paste) designed be as large...