A5015231407- Pediatric Urology and Nephrology Studies
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Advanced MRI Techniques and Applications
- MRI in cancer diagnosis
- Renal and Vascular Pathologies
- Renal and related cancers
- Iron Metabolism and Disorders
- Acute Kidney Injury Research
- Chronic Kidney Disease and Diabetes
- Metabolism and Genetic Disorders
- Trace Elements in Health
- Functional Brain Connectivity Studies
- Health, Environment, Cognitive Aging
- Organ Transplantation Techniques and Outcomes
- Neonatal Health and Biochemistry
- Nanocluster Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Advanced Image and Video Retrieval Techniques
- Neonatal and fetal brain pathology
- Robotics and Sensor-Based Localization
- Neural dynamics and brain function
- Cancer, Hypoxia, and Metabolism
- Neonatal Respiratory Health Research
- Renal cell carcinoma treatment
Washington University in St. Louis
2019-2024
Mallinckrodt (United States)
2019-2024
OPX Biotechnologies (United States)
2024
University of Hawaiʻi at Mānoa
2013-2019
Arizona State University
2014
Nephron number (N(glom)) and size (V(glom)) are correlated with risk for chronic cardiovascular kidney disease may be predictive of renal allograft viability. Unfortunately, there no techniques to assess N(glom) V(glom) in intact kidneys. This work demonstrates the use cationized ferritin (CF) as a magnetic resonance imaging (MRI) contrast agent measure viable human kidneys donated science. The were obtained from patients varying levels disease. CF was intravenously injected into three A...
number is highly variable in humans and thought to play an important role renal health. Chronic kidney disease (CKD) the result of too few nephrons maintain homeostasis. Currently, nephron can only be determined invasively or as a terminal assessment. Due lack tools measure track living, early stages CKD often go unrecognized, preventing intervention that might halt progression CKD. In this work, we present technique directly glomerular ( N
The identification of small structures (blobs) from medical images to quantify clinically relevant features, such as size and shape, is important in many applications. One particular application explored here the automated detection kidney glomeruli after targeted contrast enhancement magnetic resonance imaging. We propose a computationally efficient algorithm, termed Hessian-based Difference Gaussians (HDoG), segment blobs (e.g. kidney) 3D based on local convexity, intensity shape...
The development of chronic kidney disease (CKD) is associated with the loss functional nephrons. However, there are no methods to directly measure nephron number in living subjects. Thus, track early stages progressive CKD before changes total renal function. In this work, we used cationic ferritin-enhanced magnetic resonance imaging (CFE-MRI) enable measurements glomerular ( N glom ) and apparent volume (aV vivo healthy wild-type (WT) mice n = 4) oligosyndactylism (Os /+ ; 4), a model...
Inorganic doping was used to create flexible, paramagnetic nanoparticle contrast agents for in vivo molecular magnetic resonance imaging (MRI) with low transverse relaxivity ( r 2 ). Most formed from superparamagnetic metal oxides are developed high . While sensitive, they can have limited detection due a number of constraints T or *‐weighted imaging. 1 ‐weighted is often preferred MRI, but most ‐shortening small chelates payload nanoparticles that also shorten and limit the range...
Abstract Acute kidney injury (AKI) in premature neonates is common due to the administration of life‐saving therapies. The impact AKI on renal morphology and susceptibility further damage poorly understood. Recent advances radiological imaging have allowed integration soft tissue intact organ, facilitating a more complete understanding changes microstructure associated with pathology. Here, we applied magnetic resonance (MRI) detect both glomerular vascular rabbit model neonatal AKI, induced...
Kidney pathologies are often highly heterogeneous. To comprehensively understand kidney structure and pathology, it is critical to develop tools map tissue microstructure in the context of whole, intact organ. Magnetic resonance imaging (MRI) can provide a unique, three-dimensional view allows for measurements multiple pathological features. Here, we developed platform systematically render gross microstructural features human based on MRI. These include pyramid number morphology as well...
Accumulating evidence supports an association between nephron number and susceptibility to kidney disease. However, it is not yet possible directly measure in a clinical setting. Recent studies have used glomerular density from single biopsy whole cortical volume imaging estimate filtration rate. the accuracy of these estimates individual subjects unknown. Furthermore, clear how sample size or location may influence estimates. These questions are critical study design, potential translation...
Nephron number varies widely in humans. A low nephron endowment at birth or a loss of functioning nephrons is strongly linked to increased susceptibility chronic kidney disease. In this work, we developed contrast agent, radiolabeled cationic ferritin (RadioCF), map glomeruli vivo the using positron emission tomography (PET). PET radiotracers can be detected trace doses (<30 nmol), making them useful for rapid clinical translation. RadioCF formed from (CF) and with radioisotope, Cu-64,...
Glomerular volume is an important metric reflecting glomerular filtration surface area within the kidney. hypertrophy, or increased volume, may be marker for renal stress. Current stereological techniques report average (AVglom) These cannot assess spatial regional heterogeneity common in developing pathology. Here, we a novel "unfolding" technique to measure actual distribution of individual volumes kidney from two-dimensional glomerulus profiles observed by optical microscopy. The...
Abstract Background A significant barrier to biomarker development in the field of acute kidney injury (AKI) is use function identify candidates. Progress imaging technology makes it possible detect early structural changes prior a decline function. Early identification those who will advance chronic disease (CKD) would allow for initiation interventions halt progression. The goal this study was phenotype defined by magnetic resonance and histology discovery during transition from AKI CKD....
This work demonstrates the development and use of resting-state MRI to detect low-frequency, spontaneous physiological fluctuations in kidney consistent with previously observed perfusion potentially due autoregulatory function. These are detectable rat human kidneys, power these is affected by diabetic nephropathy rats.
In this work, we develop recombinant human cationic ferritin (rHCF) as a contrast agent to detect glomeruli in the kidney using positron emission tomography (PET). We first expressed (rHF)