A5002902508- Pediatric Urology and Nephrology Studies
- MRI in cancer diagnosis
- Advanced MRI Techniques and Applications
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Medical Image Segmentation Techniques
- Advanced Neuroimaging Techniques and Applications
- Renal and related cancers
- Iron Metabolism and Disorders
- Nanoparticle-Based Drug Delivery
- Chronic Kidney Disease and Diabetes
- AI in cancer detection
- Renal and Vascular Pathologies
- Acute Kidney Injury Research
- Trace Elements in Health
- Advanced Neural Network Applications
- Lanthanide and Transition Metal Complexes
- Characterization and Applications of Magnetic Nanoparticles
- Cell Image Analysis Techniques
- Neonatal Respiratory Health Research
- Neonatal Health and Biochemistry
- Folate and B Vitamins Research
- MicroRNA in disease regulation
- Renal function and acid-base balance
- Advanced Image and Video Retrieval Techniques
Washington University in St. Louis
2013-2024
Mallinckrodt (United States)
2019-2024
University of Hawaiʻi at Mānoa
2013-2019
Arizona State University
2009-2016
University of Hawaii System
2014
University of Virginia Medical Center
2013
Phoenix (United States)
2010
St. Joseph's Hospital and Medical Center
2009
Neurological Surgery
2009
Barrow Neurological Institute
2009
Abstract Experience with diffusion‐weighted imaging (DWI) shows that signal attenuation is consistent a multicompartmental theory of water diffusion in the brain. The source this so‐called nonexponential behavior topic debate, because cerebral cortex contains considerable microscopic heterogeneity and therefore difficult to model. To account for understand its implications current models diffusion, stretched‐exponential function was developed describe diffusion‐related decay as continuous...
Abstract The integrity of the basement membrane is essential for tissue cellular growth and often altered in disease. In this work a method noninvasively detecting structural membrane, based on delivery cationic iron‐oxide nanoparticles, was developed. Cationic particles accumulate due to highly negative charge proteoglycans membrane. kidney used test technique because its fenestrated endothelia well‐established disease models manipulate barrier. After systemic injection or native ferritin...
The goal of this work was to nondestructively measure glomerular (and thereby nephron) number in the whole kidney. Variations and size glomeruli have been linked many renal systemic diseases. Here, we develop a robust magnetic resonance imaging (MRI) technique based on injection cationic ferritin (CF) produce an accurate measurement individual glomeruli. High-field (19 Tesla) gradient-echo MR images perfused rat kidneys after vivo intravenous CF showed specific labeling with throughout We...
Nephron number (N(glom)) and size (V(glom)) are correlated with risk for chronic cardiovascular kidney disease may be predictive of renal allograft viability. Unfortunately, there no techniques to assess N(glom) V(glom) in intact kidneys. This work demonstrates the use cationized ferritin (CF) as a magnetic resonance imaging (MRI) contrast agent measure viable human kidneys donated science. The were obtained from patients varying levels disease. CF was intravenously injected into three A...
Recent advances in medical imaging technology have greatly enhanced imaging-based diagnosis which requires computational effective and accurate algorithms to process the images (e.g., measure objects) for quantitative assessment. In this research, we are interested one type of objects: small blobs. Examples blob objects cells histopathology images, glomeruli MR etc. This problem is particularly challenging because blobs often homogeneous intensity distribution an indistinct boundary against...
number is highly variable in humans and thought to play an important role renal health. Chronic kidney disease (CKD) the result of too few nephrons maintain homeostasis. Currently, nephron can only be determined invasively or as a terminal assessment. Due lack tools measure track living, early stages CKD often go unrecognized, preventing intervention that might halt progression CKD. In this work, we present technique directly glomerular ( N
Abstract The α diffusion‐weighted imaging ( DWI ) method was developed to study heterogeneous water diffusion in the human brain using magnetic resonance (MRI). An advantage of this model is that it does not require an assumption about shape intravoxel distribution apparent rates, and has a calculable relationship distribution. α‐ technique useful for detecting microstructural tissue changes associated with tumor invasion, may be directing therapy invading cells. In previous work, performed...
Techniques to measure morphological parameters, such as glomerular (and thereby nephron) number, size, and kidney volume, have been vital understanding factors contributing chronic disease (CKD). These techniques also important the associations between CKD other systemic cardiovascular diseases led identification of developmental risk for these pathologies. However, existing in quantitative morphology are resource- time-consuming destructive organ. This review discusses emerging generation...
The identification of small structures (blobs) from medical images to quantify clinically relevant features, such as size and shape, is important in many applications. One particular application explored here the automated detection kidney glomeruli after targeted contrast enhancement magnetic resonance imaging. We propose a computationally efficient algorithm, termed Hessian-based Difference Gaussians (HDoG), segment blobs (e.g. kidney) 3D based on local convexity, intensity shape...
Abstract Imaging biomarkers are being rapidly developed for early diagnosis and staging of disease. The development these requires advances in both image acquisition analysis. Detecting segmenting objects from images often the first steps quantitative measurement biomarkers. challenges detecting images, particularly small known as blobs, include low resolution, noise overlap between blobs. Difference Gaussian (DoG) detector has been used to overcome blob detection. However, DoG is...
To assess the feasibility of imaging deep-lying internal organs at high spatial resolution by kidney glomeruli in a rodent model with use newly developed, wireless amplified nuclear magnetic resonance (MR) detector.This study was approved Animal Care and Use Committee National Institutes Health/National Institute Neurologic Disorder Stroke. As preclinical demonstration this new detection technology, five different millimeter-scale MR detectors configured as double frequency resonators were...
Abstract The hypothesis was tested that the intravoxel distribution of water diffusion rates, as measured with a stretched‐exponential model diffusion‐weighted imaging (DWI), is marker brain tumor invasion. Eight rats underwent intracerebral inoculation C6 glioma cells. In three rats, cells were labeled fluorescent dye for microscopy. One rat inoculated saline solution, and five more imaged without controls. Five healthy uninoculated also imaged. DWI performed 14–15 days after inoculation,...
The development of chronic kidney disease (CKD) is associated with the loss functional nephrons. However, there are no methods to directly measure nephron number in living subjects. Thus, track early stages progressive CKD before changes total renal function. In this work, we used cationic ferritin-enhanced magnetic resonance imaging (CFE-MRI) enable measurements glomerular ( N glom ) and apparent volume (aV vivo healthy wild-type (WT) mice n = 4) oligosyndactylism (Os /+ ; 4), a model...
Abstract Magnetoferritin nanoparticles have been developed as high‐relaxivity, functional contrast agents for MRI. Several previous techniques relied on unloading native ferritin and re‐incorporation of iron into the core, often resulting in a polydisperse sample. Here, simplified technique is using commercially available horse spleen apoferritin to create monodisperse magnetoferritin. Iron oxide atoms were incorporated protein core via step‐wise Fe(II)Chloride addition solution under low O...
Abstract The goal of the work was to establish toxicity and biodistribution superparamagnetic protein cationized ferritin (CF) after intravenous injection. Intravenously injected CF has been used target extracellular matrix with high specificity in kidney glomerulus, allowing measurements individual glomeruli using T ‐weighted MRI. For routine use as an matrix‐specific tracer, it is important determine whether toxic. In this work, we investigated renal hepatic toxicity, leukocyte count,...