A5053791142- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Vestibular and auditory disorders
- Photoreceptor and optogenetics research
- Hearing, Cochlea, Tinnitus, Genetics
- Ion channel regulation and function
- Cellular transport and secretion
- Retinal Development and Disorders
- Sleep and Wakefulness Research
- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
- Cannabis and Cannabinoid Research
- Neuroscience of respiration and sleep
- Advanced Memory and Neural Computing
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Silk-based biomaterials and applications
- Microwave Engineering and Waveguides
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Superconducting and THz Device Technology
Harvard University
2016-2025
University of California, Davis
2021
Boston University
1996-2020
Broad Institute
2020
Max Planck Institute of Experimental Medicine
2020
Harvard University Press
2001-2012
Massachusetts Institute of Technology
2005
California Institute of Technology
1987-2005
Cellular Research (United States)
2001
Society for Neuroscience
2001
Synapses display remarkable alterations in strength during repetitive use. Different types of synapses exhibit distinctive synaptic plasticity, but the factors giving rise to such diversity are not fully understood. To provide experimental basis for a general model short-term we studied three rat brain slices at 34°C: climbing fiber Purkinje cell synapse, parallel and Schaffer collateral CA1 pyramidal synapse. These exhibited broad range responses regular Poisson stimulus trains. Depression...
Short-term facilitation is a widely observed form of synaptic enhancement that not well understood. Although presynaptic calcium has long been implicated in this process, its role unclear, particularly at synapses the mammalian brain. We tested residual free ([Ca]res) between granule cells and Purkinje rat cerebellar slices. Paired-pulse currents resulted an approximately 2.5-fold decayed with time constant 200 msec, as assessed by voltage-clamp recordings. Measurements [Ca]res using...
Short-term depression is a widespread form of use-dependent plasticity found in the peripheral and central nervous systems invertebrates vertebrates. The mechanism behind this transient decrease synaptic strength thought to be primarily result presynaptic "depletion" readily releasable neurotransmitter pool, which typically recovers with time constant few seconds. We studied dynamics recovery from at climbing fiber Purkinje cell synapse, where marked has been described previously. Climbing...
Abstract The cerebellar cortex is a well-studied brain structure with diverse roles in motor learning, coordination, cognition and autonomic regulation. However, complete inventory of cell types currently lacking. Here, using recent advances high-throughput transcriptional profiling 1–3 , we molecularly define across individual lobules the adult mouse cerebellum. Purkinje neurons showed considerable regional specialization, greatest diversity occurring posterior lobules. For several...
The optogenetic tool channelrhodopsin-2 (ChR2) is widely used to excite neurons study neural circuits. Previous studies of synapses suggest that light-evoked synaptic responses often exhibit artificial depression, which has been attributed either the inability ChR2 reliably fire presynaptic axons or elevating probability release by depolarizing boutons. Here, we compare and electrically evoked for high-frequency stimulation at three in mouse brain. At from Purkinje cells deep cerebellar...
Depolarization of cerebellar Purkinje neurons transiently suppresses IPSCs through a process known as depolarization-induced suppression inhibition (DSI). This IPSC occurs presynaptically and results from an unknown retrograde signal released cells. We recorded voltage-clamped cells in brain slices to identify the for DSI. find that DSI persists presence broad-spectrum metabotropic glutamate receptor antagonist LY341495 GABA(B) CGP55845, suggesting is not acting these receptors. However,...
Cerebellar Purkinje cells receive two distinctive types of excitatory inputs. Climbing fiber (CF) synapses have a high probability release and show paired-pulse depression (PPD), whereas parallel (PF) facilitate low release. We examined both using serial electron microscopic reconstructions in 15-d-old rats to look for anatomical correlates these differences. PF CF were distinguishable by their overall ultrastructural organization. There differences between how many sites within 1 microm...