A5067957989- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Gene Regulatory Network Analysis
- Plant Molecular Biology Research
- MicroRNA in disease regulation
- Plant responses to water stress
- Medicinal Plant Pharmacodynamics Research
- Neuroendocrine regulation and behavior
- Coastal wetland ecosystem dynamics
- Olfactory and Sensory Function Studies
- Soybean genetics and cultivation
- Hearing, Cochlea, Tinnitus, Genetics
- Neurogenesis and neuroplasticity mechanisms
- Neonatal Respiratory Health Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Vestibular and auditory disorders
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Neuroscience of respiration and sleep
- Muscle metabolism and nutrition
- Molecular Biology Techniques and Applications
- Nerve injury and regeneration
Massachusetts Institute of Technology
2024
Whitehead Institute for Biomedical Research
2024
Stanley Center for Psychiatric Research
2018-2022
Broad Institute
2018-2022
William & Mary
2017
Williams (United States)
2017
Centre de recherche Translationnelle en Médecine moléculaire
2007
Spatial positions of cells in tissues strongly influence function, yet a high-throughput, genome-wide readout gene expression with cellular resolution is lacking. We developed Slide-seq, method for transferring RNA from tissue sections onto surface covered DNA-barcoded beads known positions, allowing the locations to be inferred by sequencing. Using we localized cell types identified single-cell sequencing datasets within cerebellum and hippocampus, characterized spatial patterns Purkinje...
Abstract The cerebellar cortex is a well-studied brain structure with diverse roles in motor learning, coordination, cognition and autonomic regulation. However, complete inventory of cell types currently lacking. Here, using recent advances high-throughput transcriptional profiling 1–3 , we molecularly define across individual lobules the adult mouse cerebellum. Purkinje neurons showed considerable regional specialization, greatest diversity occurring posterior lobules. For several...
The cerebellum is a well-studied brain structure with diverse roles in motor learning, coordination, cognition, and autonomic regulation. Nonetheless, complete inventory of cerebellar cell types presently lacking. We used high-throughput transcriptional profiling to molecularly define across individual lobules the adult mouse cerebellum. Purkinje granule neurons showed considerable regional specialization, greatest diversity occurring posterior lobules. For multiple interneurons, molecular...
Summary Defining cell types requires integrating diverse measurements from multiple experiments and biological contexts. Recent technological developments in single-cell analysis have enabled high-throughput profiling of gene expression, epigenetic regulation, spatial relationships amongst cells complex tissues, but computational approaches that deliver a sensitive specific joint these datasets are lacking. We developed LIGER, an algorithm delineates shared dataset-specific features...
Summary Aging triggers several abnormalities in muscle glycolytic fibers including increased proteolysis, reactive oxygen species (ROS) production and apoptosis. Since the mitochondria are main site of substrate oxidation, ROS programmed cell death, we tried to know whether cellular disorders encountered sarcopenia due abnormal mitochondrial functioning. Gastrocnemius were extracted from adult (6 months) aged (21 male Wistar rats. Respiration parameters, opening permeability transition pore...
The brainstem preBötzinger complex (preBötC) generates the inspiratory breathing rhythm, and its core rhythmogenic interneurons are derived from Dbx1-expressing progenitors. To study neural bases of breathing, tamoxifen-inducible Cre-driver mice Cre-dependent reporters used to identify, record, perturb Dbx1 preBötC neurons. However, relationship between tamoxifen administration reporter protein expression in neurons glia has not been quantified. address this problem, we crossed that express...
Abstract The spatial organization of cells in tissue has a profound influence on their function, yet high-throughput, genome-wide readout gene expression with cellular resolution is lacking. Here, we introduce Slide-seq, highly scalable method that enables facile generation large volumes unbiased transcriptomes 10 µm resolution, comparable to the size individual cells. In RNA transferred from freshly frozen sections onto surface covered DNA-barcoded beads known positions, allowing locations...
Summary Endosperm is a key evolutionary innovation associated with the origin of angiosperms (flowering plants). This altruistic seed tissue supports growth and development embryo by mediating relationship mother plant as nutrient source to compatriot sink. The endosperm primary site gene imprinting in plants (where expression an allele depends on which parent it was inherited from) parent-specific epigenetic modifications like DNA methylation, are differentially patterned during male female...
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