A5008796571- Steroid Chemistry and Biochemistry
- Hormonal Regulation and Hypertension
- Metabolomics and Mass Spectrometry Studies
- Hormonal and reproductive studies
- Plant Molecular Biology Research
- Enzyme Structure and Function
- Genomics and Chromatin Dynamics
- Protein Structure and Dynamics
- Chemical Reactions and Isotopes
- Neuroscience and Neuropharmacology Research
- Eicosanoids and Hypertension Pharmacology
- ATP Synthase and ATPases Research
- Pharmacogenetics and Drug Metabolism
- thermodynamics and calorimetric analyses
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Photosynthetic Processes and Mechanisms
- Adenosine and Purinergic Signaling
- Bacteriophages and microbial interactions
- Protein Interaction Studies and Fluorescence Analysis
- Plant Gene Expression Analysis
- Metabolism, Diabetes, and Cancer
- Plant Stress Responses and Tolerance
- Neurological Disorders and Treatments
University of Nebraska–Lincoln
2010-2012
International Sakharov Environmental Institute
1997-2009
Max Delbrück Center
1997-2002
Humboldt-Universität zu Berlin
1996
Charité - Universitätsmedizin Berlin
1996
Institute of Bioorganic Chemistry
1986
The steroid hydroxylating system of adrenal cortex mitochondria consists the membrane-attached NADPH-dependent adrenodoxin reductase (AR), soluble one-electron transport protein (Adx), and a membrane-integrated cytochrome P450 CYP11 family. In 2.3-Å resolution crystal structure Adx·AR complex, 580 Å<sup>2</sup> partly polar surface are buried. Main interaction sites centered around Asp<sup>79</sup>, Asp<sup>76</sup>, Asp<sup>72</sup>, Asp<sup>39</sup> Adx Arg<sup>211</sup>,...
Tri-methylated H3 lysine 4 (H3K4me3) is associated with transcriptionally active genes, but its function in the transcription process still unclear. Point mutations catalytic domain of ATX1 (ARABIDOPSIS TRITHORAX1), a H3K4 methyltransferase, and RNAi knockdowns subunits AtCOMPASS–like (Arabidopsis Complex Proteins Associated Set) were used to address this question. We demonstrate that both are required for high level accumulation TBP (TATA-binding protein) Pol II at promoters requirement...
Background Changes in gene expression enable organisms to respond environmental stress. Levels of cellular lipid second messengers, such as the phosphoinositide PtdIns5P, change response a variety stresses and can modulate localization, conformation activity number intracellular proteins. The plant trithorax factor (ATX1) tri-methylates lysine 4 residue histone H3 (H3K4me3) at coding sequences, which positively correlates with transcription. Microarray analysis has identified target (WRKY70)...
Plants respond to environmental stresses by altering transcription of genes involved in the response. The chromatin modifier ATX1 regulates expression a large number genes; consequently, factors that affect activity would also influence from ATX1-regulated genes. Here, we demonstrate dehydration is such factor implicating plant's response drought. In addition, report hitherto unknown Arabidopsis gene, At3g10550, encodes phosphoinositide 3'-phosphatase related animal myotubularins (AtMTM1)....
NADPH‐dependent adrenodoxin reductase, and several diverse cytochromes P450 constitute the mitochondrial steroid hydroxylase system of vertebrates. During reaction cycle, transfers electrons from FAD reductase to heme iron catalytically active cytochrome (P450scc). A shuttle model for or an organized cluster all three components has been discussed explain electron transfer P450. Here, we characterize new covalent, zero‐length crosslinks mediated by 1‐ethyl‐3‐(3‐dimethylaminopropyl)...
Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating activity. Here, we report that novel member family has role unrelated to chromatin. It is encoded an internal in ATX1 isoform containing only SET domain (soloSET). located exclusively cytoplasm and its substrate...
NADPH-dependent adrenodoxin reductase, and several diverse cytochromes P450 constitute the mitochondrial steroid hydroxylase system of vertebrates. During reaction cycle, transfers electrons from FAD reductase to heme iron catalytically active cytochrome (P450scc). A shuttle model for or an organized cluster all three components has been discussed explain electron transfer P450. Here, we characterize new covalent, zero-length crosslinks mediated by 1-ethyl-3-(3-dimethylaminopropyl)...
Bovine adrenodoxin was cross-linked to reductase with 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide. Mass spectrometry showed the reaction product be a 1:1 complex of two proteins M(r) = 64,790 +/- 50. The cytochrome c activity and could crystallized by hanging-drop vapor diffusion. Crystals adrenodoxin-adrenodoxin are hexagonal, space group P6(1)22 or P6(5)22, 93.26 A 612.20 diffract 2.9 resolution at 100 K. Assuming complexes per asymmetric unit yields reasonable V(M) 2.97 A3/Da.
Biotechnology methods allow getting homogenous biologically active preparations in virtually unlimited quantities. Recombinant protein purification technology is being constantly improved to increase expression level and minimization of number stages. This paper introduces the new method one-stage recombinant His-Tag fusion adrenodoxin cloning. It keeps all vector advantages for its periplasmatic expression. In this issue, optimized detergents system effective lysis bacteria cells has been described.