A5035779498- Genomics, phytochemicals, and oxidative stress
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Enzyme Structure and Function
- Nitrogen and Sulfur Effects on Brassica
- Polysaccharides and Plant Cell Walls
- Moringa oleifera research and applications
- Plant tissue culture and regeneration
- Protein Structure and Dynamics
- Plant-Microbe Interactions and Immunity
- Plant Surface Properties and Treatments
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Agricultural Practices and Plant Genetics
- Spectroscopy and Chemometric Analyses
- Plant pathogens and resistance mechanisms
- Leaf Properties and Growth Measurement
- Coenzyme Q10 studies and effects
- Selenium in Biological Systems
- Horticultural and Viticultural Research
- Heat shock proteins research
Ministry of Agriculture and Rural Affairs
2022-2024
Center for Excellence in Molecular Cell Science
2016-2024
Chinese Academy of Sciences
2016-2024
Shanghai University of Traditional Chinese Medicine
2024
Zhejiang A & F University
2011-2023
Duke Medical Center
2021
Howard Hughes Medical Institute
2021
Duke University Hospital
2021
University of Chinese Academy of Sciences
2018-2019
Shanghai Institutes for Biological Sciences
2016
TRiC/CCT assists the folding of ∼10% cytosolic proteins through an ATP-driven conformational cycle and is essential in maintaining protein homeostasis. Here, we determined ensemble cryo-electron microscopy (cryo-EM) structures yeast TRiC at various nucleotide concentrations, with 4 open-state maps resolved near-atomic resolutions, a closed-state map atomic resolution, revealing extra layer unforeseen N-terminal allosteric network. We found that, during ring closure, CCT7 subunit moves first,...
Heat stress is one of the major abiotic factors that limit growth, development, and productivity plants. Both glycine betaine (GB) β-aminobutyric acid (BABA) have received considerable attention due to their roles in stimulating tolerance diverse stresses. In order understand how GB BABA biostimulants alleviate heat a cool-weather Chinese cabbage (
Three Arabidopsis cDNAs, MAM1, CYP79F1, and CYP83A1, required for aliphatic glucosinolate biosynthesis were introduced into Chinese cabbage by Agrobacterium tumefaciens-mediated transformation. The transgenic lines overexpressing MAM1 or CYP83A1 showed wild-type phenotypes. However, all the CYP79F1 displayed phenotypes different from wild type with respect to stem thickness as well leaf width shape. Glucosinolate contents of plants compared those type. In line M1-1, accumulation...
Polygalacturonase (PG), a large hydrolase family in plants, is involved pectin disassembly of the cell wall plants. The present study aims to characterize PG genes and investigate their expression patterns Solanum lycopersicum. We identified 54 tomato genome compared amino acid sequences with Arabidopsis counterpart. Subsequently, we renamed these according homologs. Phylogenetic evolutionary analysis revealed that could be classified into seven clades, within each clade exon/intron...
Brassicaceae vegetables are an important traditional daily food in China and around the world, which provide nutrients phytochemicals that beneficial for human health. Among them, Brassica Raphanus widely cultivated eaten, have been evolved and/or bred special characteristics during long history of cultivation. Epidemiological studies suggest health benefits mainly associated with glucosinolates (GSLs) their hydrolytic products. In this review, we discuss diversity common consumed GSL...
Heat stress is one of the most common agrometeorological risks in crop production middle and lower reaches Yangtze River China. This study aimed to investigate whether glutamic acid (Glu) or poly-γ-glutamic (γ-PGA) biostimulants can improve thermotolerance a cool-season Chinese cabbage (Brassica rapa L. ssp. pekinensis) crop. Priming with Glu (2.0 mM) γ-PGA (20 mg·L−1) was conducted at third leaf stage by applying as daily foliar sprays for 5 days before heat (45 °C 16-h light/35 8-h dark)....
Chinese cabbage (Brassica rapa L. ssp. pekinensis) is sensitive to high temperature, which will cause the B. remain in a semi-dormancy state. Foliar spray of GB prior heat stress was proven enhance thermotolerance. In order understand molecular mechanisms GB-primed resistance or adaptation towards stress, we investigated transcriptomes and non-primed heat-sensitive 'Beijing No. 3' variety by RNA-Seq analysis. A total 582 differentially expressed genes (DEGs) were identified from plants...
Indole glucosinolates (IG) play important roles in plant defense, plant-insect interactions, and stress responses plants. In an attempt to metabolically engineer the IG pathway flux Chinese cabbage, three Arabidopsis cDNAs, CYP79B2, CYP79B3, CYP83B1, were introduced into cabbage by Agrobacterium-meAiateA transformation. Overexpression of CYP79B3 or CYP83B1 did not affect accumulation levels, overexpression CYP79B2 prevented transformed callus from being regenerated, displaying phenotype...
Okra is a kind of flavonoid-rich food which was reported to have variety health functions. Flavonoids are the major polyphenolic compounds in okra and thought play role reducing risk disease. The aim this study isolate identify flavonoids composition pods explore activity main components identified on inhibiting tumor cell proliferation vitro. Six individual were by HPLC-MS/MS: quercetin-3-gentiobioside (Q3G), quercetin-3-sambubioside (Q3S), rutin, quercetin-7-glucoside (Q7G), isoquercitrin...
Sclerotinia stem rot caused by sclerotiorum is one of the most destructive diseases in Brassica rapa. Verticillium dahliae Aspf2-like protein (VDAL) a secretory V. which has been shown to enhance resistance against fungal infections several plants. Nonetheless, molecular mechanisms VDAL-primed disease are still poorly understood. In this study, we performed physiological, biochemical, and transcriptomic analyses rapa order understand how VDAL confers S. sclerotiorumn The results showed that...
Glossiness is an important quality-related trait of Chinese cabbage, which a leafy vegetable crop in the family Brassicaceae. The glossy caused by abnormal cuticular wax accumulation. In this study, on basis bulked segregant analysis coupled with next-generation sequencing (BSA-seq) and fine-mapping, most likely candidate gene responsible for phenotype cabbage was identified. It subsequently named Brcer2 because it homologous to AtCER2 (At4g24510). A bioinformatics indicated long...
Abstract Unambiguous subunit assignment in a multicomponent complex is critical for thorough understanding of the machinery and its functionality. The eukaryotic group II chaperonin TRiC/CCT folds approximately 10% cytosolic proteins important maintenance cellular homeostasis. TRiC consists two rings each ring has eight homologous but distinct subunits. identification macromolecular machine such as through intermediate or low-resolution cryo-EM map remains challenging. Here we present yeast...