Microalgae are a source of potentially healthy and sustainable nutrients. However, the bioaccessibility these nutrients remains uncertain. In this study, we analyzed biomass composition five commercial

The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable performing posttranslational modifications like glycosylation secreted proteins. However, activity OCH1 encoded α-1,6-mannosyltransferase triggers hypermannosylation proteins at great heterogeneity, considerably hampering downstream processing and reproducibility. Horseradish peroxidases are versatile enzymes with applications in diagnostics, bioremediation cancer treatment. Despite...

Microalgae are attractive for the food and cosmetic industries because of their nutrient composition. However, bioaccessibility extractability nutrients in microalgae limited by rigid indigestible cell wall. The goal this study is to explore wall polysaccharides (CWPSs) composition morphology heterotrophic Crypthecodinium cohnii Chlorella vulgaris biomasses during growth. Our results showed that glucose was major component CWPSs exopolysaccharides C. cohnii. have a similar sugar profile...

The polyextremophilic Galdieria sulphuraria is emerging as a promising microalgal species for food applications. This work explores the potential of heterotrophically cultivated G. protein producer human consumption. To this end, performances four strains grown under same conditions were compared. Amino acid profiles varied among and growth phases, but all samples met FAO dietary requirements adults. specific rates between 1.01 1.48 day−1. After glucose depletion, showed an increase 38–49 %...

Microalgal biomass is an emerging source of several health-related compounds, including polyunsaturated fatty acids. Herein, Chlorella vulgaris was cultivated heterotrophically in a 16-L stirred tank bioreactor. The lipid oxidative stability and bioaccessibility the harvested during exponential stationary phases were evaluated. phase showed lower oxidation than that phase, likely due to higher content antioxidants former. In both biomasses, hexanal propanal profiles only moderate increase...

Microalgae are a promising source of polyunsaturated fatty acids as well bioactive antioxidant compounds such carotenoids, phenolics and tocopherols. However, the accumulation these biomolecules is often promoted by conflicting growth conditions. In this study, phased bioprocessing strategy was developed to simultaneously enhance lipid amounts tailoring nitrogen content in cultivation medium applying light stress. This approach increased overall contents total acids, phenolics, α-tocopherol...

Determining total protein content is a routine operation in many laboratories. Despite substantial work on assay optimization interferences, the widely used bicinchoninic acid (BCA) remains recognized for its robustness. Especially field of bioprocess engineering inaccuracy caused by interfering substances hardly predictable and not well understood. Since introduction assay, sample pre-treatment trichloroacetic (TCA) precipitation has been indicated as necessary sufficient to minimize...

No AccessJournal of UrologyInvestigative Urology1 Oct 2011Lectin Mediated Biorecognition as a Novel Strategy for Targeted Delivery to Bladder Cancer Lukas Neutsch, Verena E. Plattner, Sonja Polster-Wildhofen, Agnes Zidar, Andreas Chott, Gerrit Borchard, Othmar Zechner, Franz Gabor, and Michael Wirth NeutschLukas Neutsch Department Pharmaceutical Technology Biopharmaceutics, University Vienna, Austria , PlattnerVerena Plattner Polster-WildhofenSonja Polster-Wildhofen Urology,...

The use of bioadhesive polymers as nanodevices has emerged a promising strategy for oral delivery therapeutics. In this regard, poly(anhydride) nanoparticles have shown great potential drug and vaccine purposes. However, despite extensive research into the biomedical pharmaceutical applications nanoparticles, there are no studies to evaluate interaction these at cellular level. Therefore, main objectives study were cytotoxicity well cell different nanoparticles: conventional (NP), containing...

Many vital components in bioprocess media are prone to photo-conversion or photo-degradation upon exposure ambient light, with severe negative consequences for biomass yield and overall productivity. However, there is only limited awareness of light irradiation as a potential risk factor when working transparent glass bioreactors, storage vessels disposable bag systems. The chemical complexity most renders root-cause analysis difficult. This study investigated novel, holistic approach how...

Therapeutic and diagnostic carriers can be functionalized with active targeters to induce tissue-specific delivery. However, the possible impact of adsorbed steric stabilizer such as frequently used poloxamers (Pluronics) on surface modification poly(D,L-lactide-co-glycolide) (PLGA) particles has not been examined so far. Therefore, three model ligands different molecular weights (653; 36,000; 155,000 g/mol) covering size range important were conjugated PLGA microparticles in presence...

Iron is crucial for bacterial growth and virulence. Under iron-deficiency bacteria produce siderophores, iron chelators that facilitate the uptake into cell via specific receptors. Erwinia amylovora, causative agent of fire blight, produces hydroxamate-type desferrioxamine siderophores (DFO). The presented study reassesses impact DFO as a virulence factor E. amylovora during its epiphytic phase on apple flower. When inoculated in semisterile Golden Delicious flowers no difference replication...

Monitoring of bioprocesses, especially biopharmaceutical production processes, is utmost importance to ensure constant product quality and availability. Economical bioprocess development depends on time-efficient process development, aiming for in-depth understanding the its critical parameters. Online monitoring bioprocesses allows assessment processing data in real time. This a prerequisite permit real-time management. Various devices automated sampling analysis, as well analysis...

Urinary tract infections (UTIs) are among the most common bacterial infections. In an increasing number of cases, pathogen (multi-)resistance hampers durable treatment success via standard therapies. On functional level, activity urinary excreted antibiotics is compromized by efficient tissue colonization mechanism uropathogenic Escherichia coli (UPEC). Advanced drug delivery systems aim at exploiting a glycan-mediated targeting mechanism, similar to UPEC invasion pathway, increase...