A5001797311- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- RNA Research and Splicing
- RNA modifications and cancer
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Animal Disease Management and Epidemiology
- RNA Interference and Gene Delivery
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Viral gastroenteritis research and epidemiology
- Insect Resistance and Genetics
- Genomics and Chromatin Dynamics
- Animal Virus Infections Studies
- Polyamine Metabolism and Applications
- Probiotics and Fermented Foods
- Telomeres, Telomerase, and Senescence
- RNA regulation and disease
- Polymer Surface Interaction Studies
- CRISPR and Genetic Engineering
- Studies on Chitinases and Chitosanases
- interferon and immune responses
- Genomics and Phylogenetic Studies
National Institute of Advanced Industrial Science and Technology
2008-2025
The University of Tokyo
2006-2014
Biomed Research Institute
2012
Nestin is a type VI intermediate filament protein and well-known neural stem cell marker. It also expressed in high-grade cancer cells, forming copolymerized filaments with vimentin. We previously showed that nestin inhibits the binding of vimentin's tail domain to actin (AFs) by steric hindrance through its large (NTD), thereby increasing three-dimensional cytoskeleton network mobility, enhancing flexibility, promoting progression. Further, we found itself stably binds AFs via NTD....
Summary Viral infection triggers the activation of host innate immune responses, including integrated stress response (ISR). The antiviral is mediated by phosphorylation eukaryotic translation initiation factor 2 alpha (eIF2α) catalyzed eIF2α kinase, resulting in suppression global protein synthesis and induction selected genes cell. To evade and/or subvert signalling pathway, viruses have evolved harnessed elaborate counter-attack mechanisms to repress kinase. In insects, kinase-mimic PK2...
Replication and transcription of viral RNA genomes rely on host-donated proteins. Qβ virus infects Escherichia coli replicates transcribes its own genomic by replicase. replicase requires the virus-encoded RNA-dependent polymerase (β-subunit), translational elongation factors EF-Tu -Ts, as active core subunits for polymerization activity. Here, we present crystal structure replicase, comprising β-subunit, -Ts. The β-subunit has a right-handed structure, EF-Tu:Ts binary complex maintains...
Ribosomal protein S1, consisting of six contiguous OB-folds, is the largest ribosomal and essential for translation initiation in Escherichia coli. S1 also one three host-derived subunits Qβ replicase, together with EF-Tu EF-Ts, RNA replication E. We analyzed crystal structure virus-encoded RNA-dependent polymerase (β-subunit), EF-Tu, EF-Ts N-terminal half which capable initiating replication. Structural biochemical studies revealed that two OB-folds anchor onto β-subunit, third OB-fold...
Abstract Saccharomyces cerevisiae Est1p is a telomerase‐associated protein essential for telomere length homeostasis. hEST1A one of the three human homologues and considered to be involved not only in regulation elongation or capping but also nonsense‐mediated degradation RNA. composed two conserved regions, homology PIN ( Pi lT N ‐terminus) domains. The present study shows crystal structure domain at 1.8 Å resolution. overall an α/β fold core similar counterpart 5′ nucleases extended absent...
Abstract Troponin C (TnC) is the Ca 2+ -sensing subunit of troponin that triggers contraction striated muscles. In scallops, muscles consume little ATP energy in sustaining strong contractile forces. The N-terminal domain TnC works as sensor vertebrates, whereas scallop uses C-terminal sensor, suggesting there are differences mechanism -dependent regulation between invertebrates and vertebrates. Here, we report crystal structure Akazara ( Chlamys nipponensis akazara ) adductor muscle (asTnC...
Chiral molecule (R)-3-quinuclidinol, a valuable compound for the production of various pharmaceuticals, is efficiently synthesized from 3-quinuclidinone by using NADPH-dependent reductase (RrQR) Rhodotorula rubra. Here, we report crystal structure RrQR and structure-based mutational analysis. The enzyme forms tetramer, in which core each protomer exhibits α/β Rossmann fold contains one NADPH, whereas characteristic substructures small lobe variable loop are localized around substrate-binding...
(R)-3-Quinuclidinol is a useful chiral building block for the synthesis of various pharmaceuticals and can be produced from 3-quinuclidinone by asymmetric reduction. A novel reductase Agrobacterium tumefaciens (AtQR) catalyzes stereospecific reduction to (R)-3-quinuclidinol with NADH as cofactor. Recombinant AtQR was overexpressed in Escherichia coli, purified crystallized using sitting-drop vapour-diffusion method at 293 K. Crystals were obtained reservoir solution containing PEG 3350...
Human EST1A (ever shorter telomeres 1A) is associated with most or all active telomerase in cell extracts and involved either directly indirectly telomere elongation capping. The C-terminal region of contains the PIN (PilT N-terminus) domain, a putative nuclease domain. domain human was expressed, purified crystallized by sitting-drop vapour-diffusion method. crystals belonged to space group C2, unit-cell parameters = 107.3, b 51.6, c 100.5 angstroms, beta 119.3 degrees, diffracted X-rays...
(R)-3-Quinuclidinol is a useful compound that applicable to the synthesis of various pharmaceuticals. The NADPH-dependent carbonyl reductase 3-quinuclidinone from Rhodotorula rubra catalyzes stereospecific reduction (R)-3-quinuclidinol and expected be utilized in industrial production this alcohol. 3-Quinuclidinone R. was expressed Escherichia coli purified using Ni-affinity ion-exchange column chromatography. Crystals protein were obtained by sitting-drop vapour-diffusion method PEG 8000 as...
Human Dicer protein contains two RNase III domains (RNase IIIa and IIIb) which are involved in the production of short interfering RNAs (siRNAs). The C-terminal domain human was expressed, purified crystallized by sitting-drop vapour-diffusion method. crystals belonged to space group C2221, with unit-cell parameters a = 88.6, b 199.7, c 119.6 Å, diffracted X-rays 2.0 Å resolution. asymmetric unit contained three molecules IIIb solvent content 67%.
Phosphorylation of the α subunit eukaryotic translation initiation factor 2 (eIF2α) by eIF2α kinases is a common mechanism to regulate under stress conditions. The PK2 protein from baculovirus Autographica californica multiple nucleopolyhedrovirus (AcMNPV) binds and inhibits ensure efficient virus propagation. C-terminal region shares homology with C-lobe kinases, but N-terminal unique orthologous proteins. In order understand detailed structure function PK2, both full-length its truncated...
Abstract Periplasmic solute-binding proteins (SBPs) specific for chitooligosaccharides, (GlcNAc) n (n = 2, 3, 4, 5 and 6), are involved in the uptake of chitinous nutrients negative control chitin signal transduction Vibrios . Most translocation processes by SBPs across inner membrane have been explained thus far two-domain open/closed mechanism. Here we propose three-domain mechanism based on experiments using a recombinant Vc CBP, SBP from Vibrio cholerae X-ray crystal structures...