A5082676016- Chromatin Remodeling and Cancer
- Retinoids in leukemia and cellular processes
- Protein Degradation and Inhibitors
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Research on Leishmaniasis Studies
- Hematopoietic Stem Cell Transplantation
- Acute Lymphoblastic Leukemia research
- Erythrocyte Function and Pathophysiology
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Microtubule and mitosis dynamics
- Diet and metabolism studies
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- RNA regulation and disease
- CAR-T cell therapy research
- Dietary Effects on Health
Baylor College of Medicine
2021-2025
Texas Children's Hospital
2021-2024
AstraZeneca (United States)
2024
Haematopoietic stem cells maintain blood production throughout life1. Although extensively characterized using the laboratory mouse, little is known about clonal selection and population dynamics of haematopoietic cell pool during murine ageing. We isolated progenitors from young old mice, identifying 221,890 somatic mutations genome-wide in 1,845 single-cell-derived colonies. Mouse accrue approximately 45 per year, a rate only threefold greater than human despite vastly different organismal...
Abstract In acute myeloid leukemia (AML), SWI/SNF chromatin remodeling complexes sustain leukemic identity by driving high levels of MYC. Previous studies have implicated the hematopoietic transcription factor PU.1 (SPI1) as an important target inhibition, but is widely regarded to pioneer-like activity. As a result, many questions remained regarding interplay between and in AML well normal hematopoiesis. Here we found that binds most its targets SWI/SNF-independent manner recruits promote...
During aging, blood cell production becomes dominated by a limited number of variant hematopoietic stem (HSC) clones. Differentiated progeny HSCs are thought to mediate the detrimental effects such clonal hematopoiesis on organismal health, but mechanisms poorly understood. While somatic mutations in
No method has been developed for single-cell analysis of the large repositories preserved whole blood samples stored in PAXgene Blood RNA tubes. To address this gap, two nuclei isolation techniques single-nucleus sequencing were evaluated: mechanical separation (MS), using an Acrodisc filter, and cell lysis (CL). While both methods captured from all major immune types, CL resulted up to orders magnitude higher yields less biased proportions cells than MS. High ambient globin gene counts...
Tatton-Brown-Rahman syndrome (TBRS) is an overgrowth disorder caused by germline heterozygous mutations in the DNA methyltransferase DNMT3A. DNMT3A a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic are frequent hematologic malignancies clonal hematopoiesis. Yet, impact constitutive mutation on hematopoiesis TBRS undefined. In order to establish how impacts blood development we gathered clinical data analyzed parameters 18 individuals with TBRS. We also...
Abstract Haematopoietic stem cells maintain blood production throughout life. While extensively characterised using the laboratory mouse, little is known about how population sustained and evolves with age. We isolated progenitors from young old mice, identifying 221,890 somatic mutations genome-wide in 1845 single cell-derived colonies, used phylogenetic analysis to infer ontogeny dynamics of cell pool. Mouse accrue ∼45 per year, a rate only 2-fold greater than human despite vastly...
IKAROS family zinc finger 1 (IKZF1) alterations represent a diverse group of genetic lesions that are associated with an increased risk relapse in B-cell acute lymphoblastic leukemia. Due to the heterogeneity concomitant lesions, it remains unclear how IKZF1 abnormalities directly affect cell function and therapy resistance, whether their consideration as prognostic indicator is valuable improving outcome. CRISPR/Cas9 strategies were used engineer multiple panels isogeneic lymphoid leukemia...
Abstract Breast cancer is one of the most diagnosed cancers and has a complex tumor microenvironment (TME). tumors are subtyped by receptor expression, all have high degree cellular heterogeneity. We analyzed from 8 individuals with early-stage breast cancer. The patients had either triple negative (TNBC) or an estrogen (ER) positive normal adjacent tissue were profiled single-nuclei gene expression (GEX) ATAC data using 10X multi-ome methodology. Gene set analysis identified specific...
7033 Background: Approximately 20% of children diagnosed with B lymphoblastic leukemia (B-ALL) will suffer a relapse, and most adults B-ALL have poor prognosis. Genome-wide association studies patients identified frequent deletions the gene IKZF1, encoding master regulator lymphoid development, IKAROS. These are associated therapy resistance, increased risk inferior survival. Currently, how loss IKAROS function contributes to resistance relapse is not fully understood. We used CRISPR-Cas9...
<div>Abstract<p>In acute myeloid leukemia (AML), SWI/SNF chromatin remodeling complexes sustain leukemic identity by driving high levels of MYC. Previous studies have implicated the hematopoietic transcription factor PU.1 (SPI1) as an important target inhibition, but is widely regarded to pioneer-like activity. As a result, many questions remained regarding interplay between and in AML well normal hematopoiesis. Here we found that binds most its targets SWI/SNF-independent manner...
<p>Supplementary Materials</p>
<p>Complete blood counts</p>
<p>RNA-seq datasets</p>
<p>Gene set enrichment analyses</p>
<p>RNA-seq datasets</p>
<p>Gene set enrichment analyses</p>
<p>Complete blood counts</p>
<p>Complete blood counts</p>
<p>Motif profiling</p>
<p>Supplementary Materials</p>
<p>Gene set enrichment analyses</p>
<p>Motif profiling</p>
<p>Gene set enrichment analyses</p>