- Biofuel production and bioconversion
- Sugarcane Cultivation and Processing
- Polysaccharides and Plant Cell Walls
- Food composition and properties
- Natural Products and Biological Research
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Lignin and Wood Chemistry
- Microbial Natural Products and Biosynthesis
- Transgenic Plants and Applications
- Seed Germination and Physiology
- Chromatography in Natural Products
- Analytical Chemistry and Chromatography
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Anaerobic Digestion and Biogas Production
Norwegian University of Life Sciences
2019-2021
Universidade de São Paulo
2013-2016
Biotech (Brazil)
2014
Universidade Federal de Viçosa
2011
Abstract The saccharification of lignocellulosic materials like Norway spruce is challenging due to the recalcitrant nature biomass, and it requires optimized efficient pretreatment enzymatic hydrolysis processes make industrially feasible. In this study, we report successful sulfite‐pulped (Borregaard's BALI™ process) at demonstration scale, achieved through controlled delivery hydrogen peroxide (H 2 O ) for activation lytic polysaccharide monooxygenases (LPMOs) present in cellulolytic...
Sugar cane internodes can be divided diagonally into four fractions, of which the two innermost ones are least recalcitrant pith and moderately accessible pith-rind interface. These fractions differ in enzymatic hydrolyzability due to structural differences. In general, cellulose hydrolysis plants is hindered by its physical interaction with hemicellulose lignin. Lignin believed linked covalently through hydroxycinnamic acids, forming a compact matrix around polysaccharides. Acetyl xylan...
Grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim produce transportation fuels and a variety of chemicals. However, polysaccharides contained in grass cell walls built recalcitrant composite. Deconstruction these is still challenge energy-efficient economically viable transformation materials. The varied tissue-specific distribution wall components adds complexity origins recalcitrance grasses. This usually led...
Abstract Background The recent discovery that LPMOs can work under anaerobic conditions when supplied with low amounts H 2 O opens the possibility of using as enzyme aids in biogas reactors to increase methane yields from lignocellulosic materials. We have explored this by studying digestion various materials: Avicel, milled spruce and birch wood, a lignin-rich hydrolysis residue steam-exploded birch. digestions were added cellulolytic cocktails carried out or without addition . Results In...
Research on oligosaccharides, including the complicated product mixtures generated by lytic polysaccharide monooxygenases (LPMOs), is growing at a rapid pace. LPMOs are gaining major interest, and ability to efficiently accurately separate quantify their native oxidized products chromatographically essential in furthering our understanding of these oxidative enzymes. Here we present novel set methods based dual electrolytic eluent generation, where conventional sodium acetate/sodium...