A5029886646- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- ATP Synthase and ATPases Research
- Bacterial biofilms and quorum sensing
- Cancer, Hypoxia, and Metabolism
- Bacterial Identification and Susceptibility Testing
- Genetics, Aging, and Longevity in Model Organisms
- Advanced biosensing and bioanalysis techniques
- Plant-Microbe Interactions and Immunity
- Biosensors and Analytical Detection
- Gene Regulatory Network Analysis
- Machine Learning and Algorithms
- Streptococcal Infections and Treatments
- SARS-CoV-2 detection and testing
- Collaboration in agile enterprises
- Erythrocyte Function and Pathophysiology
- semigroups and automata theory
- Antibiotic Resistance in Bacteria
- Yeasts and Rust Fungi Studies
- Advanced Statistical Process Monitoring
- Microbial infections and disease research
- Microbial Community Ecology and Physiology
Institute for Stem Cell Biology and Regenerative Medicine
2016-2024
Augusta University
2023-2024
Academy of Scientific and Innovative Research
2024
Centre for Cellular and Molecular Biology
2022-2024
University of Kansas
2014-2021
ORCID
2020-2021
National Centre for Biological Sciences
2016
Kansas State University
2012-2013
Indian Institute of Science Bangalore
1973
Abstract The COVID-19 pandemic has strained testing capabilities worldwide. There is an urgent need to find economical and scalable ways test more people. We present Tapestry , a novel quantitative nonadaptive pooling scheme many samples using only few tests. underlying molecular diagnostic any real-time RT-PCR panel approved for the detection of SARS-CoV-2 virus. In cases where most are negative virus, accurately identifies status each individual sample with single round in fewer tests than...
Enterococcus faecalis is a commensal bacterium found in the gastrointestinal tract of most mammals, including humans, and one leading causes nosocomial infections. One hallmarks E. pathogenesis its unusual ability to tolerate high concentrations lysozyme, which an important innate immune component host. Previous studies have shown that presence lysozyme leads activation SigV, extracytoplasmic function (ECF) sigma factor faecalis, deletion sigV increases susceptibility toward lysozyme. Here,...
How phenotypically distinct states in isogenic cell populations appear and stably co-exist remains unresolved. We find that within a mature, clonal yeast colony developing low glucose, cells arrange into metabolically disparate groups. Using this system, we model experimentally identify metabolic constraints sufficient to drive such self-assembly. Beginning uniformly gluconeogenic state, exhibiting contrary, high pentose phosphate pathway activity spontaneously proliferate, spatially...
Multidrug-resistant Enterococcus faecalis possess numerous mobile elements that encode virulence and antibiotic resistance traits as well new metabolic pathways, often constituting over one-quarter of the genome. It was interest to determine how this large accretion affects competitive growth in gastrointestinal (GI) tract consortium. We unexpectedly observed prototype clinical isolate strain V583 actively killed by GI flora, whereas commensal enterococci flourished. found killing resulted...
Enterococcus faecalis V583 is a vancomycin-resistant clinical isolate which belongs to the hospital-adapted clade, CC2. This strain harbours several factors that have been associated with virulence, including fsr quorum-sensing regulatory system known control expression of GelE and SprE proteases. To discriminate between genes directly regulated by Fsr, those indirectly as result protease or activity, we compared gene in isogenic mutants variously defective either Fsr quorum sensing...
Enterococci are leading causes of hospital-acquired infection in the United States and continue to develop resistances new antibiotics. Many Enterococcus faecalis isolates harbor pheromone-responsive plasmids that mediate horizontal transfer even large blocks chromosomal genes, resulting hospital-adapted strains over a quarter whose genomes consist mobile elements. Pheromones which donor cells respond derive from lipoprotein signal peptides. Using novel bacterial killing assay dependent on...
Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing ∆ubp3 cells, orthogonal approaches,...
Previously, we found that in glucose-limited Saccharomyces cerevisiae colonies, metabolic constraints drive cells into groups exhibiting gluconeogenic or glycolytic states. In study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization state, serving as carbon source fuels glycolysis (Varahan et al., 2019). We now discover plasticity use non-limiting resource, aspartate, both resource production heterogeneous cell states, based...
Enterococcus faecalis causes health care-associated infections and displays resistance to a variety of antibiotics molecules the innate immune system. SigV has been shown play an important role in enterococcal lysozyme resistance.
Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing ∆ubp3 cells, orthogonal approaches,...
The alternative sigma factor σ54 has been shown to regulate the expression of a wide array virulence-associated genes, as well central metabolism, in bacterial pathogens. In Gram-positive organisms, is commonly associated with carbon metabolism. this study, we show that Enterococcus faecalis (RpoN) and its cognate enhancer binding protein MptR are essential for mannose utilization primary contributors glucose uptake through Mpt phosphotransferase system. To gain further insight into how RpoN...
Enterococcus faecalis is an opportunistic pathogen capable of causing infections, including endocarditis and urinary tract infections (UTI). One the well-characterized quorum-sensing pathways in E. involves coordination conjugal transfer pheromone-responsive plasmids by PrgX, a member RRNPP protein family. Members this family various Firmicutes have also been shown to contribute numerous cellular processes, sporulation, competence, conjugation, nutrient sensing, biofilm formation, virulence....
Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal approaches,...
Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal approaches,...
Abstract Previously, we discovered that in glucose-limited yeast colonies, metabolic constraints drive cells into groups exhibiting gluconeogenic and glycolytic states. Here, threshold amounts of trehalose - a limiting, produced resource, controls the emergence self-organization state, by acting as carbon source to fuel these demands (Varahan et al., 2019). We now discover plasticity use non-limiting aspartate, both resource production heterogeneous cell states, based on differential...
Abstract We have previously described Tapestry Pooling, a scheme to enhance the capacity of RT-qPCR testing, and provided experimental evidence with spiked synthetic RNA show that it can help scale testing restart economy. Here we report on validation studies Covid19 patient samples for Pooling prevalence in range 1% 2%. pooled extracted from were tested Covid19, sending each sample three pools. Following different pooling schemes, pipetted 320 into 48 pools pool size 20 at rate 1.6%, 500 60...
Abstract Many cells in high glucose repress mitochondrial respiration, as observed the Crabtree and Warburg effects. Our understanding of biochemical constraints for activation is limited. Using a Saccharomyces cerevisiae screen, we identified conserved deubiquitinase Ubp3 (Usp10), necessary repression. mutants have increased activity despite abundant glucose, along with decreased glycolytic enzymes, rewired metabolic network trehalose production. Utilizing Δubp3 cells, orthogonal...