A5079931881- Hemophilia Treatment and Research
- Protein purification and stability
- Prion Diseases and Protein Misfolding
- Cardiac electrophysiology and arrhythmias
- Cardiac pacing and defibrillation studies
- Cardiac Arrhythmias and Treatments
- Hemostasis and retained surgical items
- Blood Coagulation and Thrombosis Mechanisms
- Atrial Fibrillation Management and Outcomes
- Blood groups and transfusion
- nanoparticles nucleation surface interactions
- Parvovirus B19 Infection Studies
- Blood donation and transfusion practices
- Viral Infectious Diseases and Gene Expression in Insects
- Fluid Dynamics and Mixing
- Proteins in Food Systems
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Cardiovascular Function and Risk Factors
- Non-Invasive Vital Sign Monitoring
- Freezing and Crystallization Processes
- Blood properties and coagulation
- Cardiac Imaging and Diagnostics
- Respiratory Support and Mechanisms
The Alan Turing Institute
2024
Scottish National Blood Transfusion Service
1997-2013
Methodist Hospital
1990-2001
Nashville Oncology Associates
2001
Indiana University – Purdue University Indianapolis
1975-1999
Houston Methodist
1999
Florida SouthWestern State College
1999
Tufts University
1988-1993
Element Six (United States)
1987
Indiana University School of Medicine
1977-1981
Abstract The freeze denaturation of model proteins, LDH, ADH, and catalase, was investigated in absence cryoprotectants using a microcryostage under well‐controlled freezing thawing rates. Most the experimental data were obtained from study dilute solution with an enzyme concentration 0.025 g/l. dependence activity recovery proteins on rates showed reciprocal independent effect, that is, slow (at rate about 1°C/min) fast >10°C/min) produced higher recovery, whereas resulted more severe...
Changes in the ventricular diastolic excitability threshold following occlusion of left anterior descending coronary artery (LAD) were studied open-chest anesthetized dogs by using a new automatic threshold-following pacemaker (ATFP). The ATFP measures successively decreasing duration regularly occurring pacing stimuli until ventricle fails to respond. Under control conditions, stimulus was 60 +/- 4 (mean SEM) musec. In first 1-3 minutes LAD, ischemic zone (IZ) decreased 51 5 musec and then...
In this study, we describe the findings in 18 young patients (age range 4 days to 24 years, mean 16.6 years) who had ventricular tachycardia and/or fibrillation and were followed for 4--70 months (mean 22.4 months). Patients a variety of problems associated with their arrhythmia, including mitral valve prolapse, cardiomyopathy, myocarditis, prolonged QT syndrome hypokalemia. Six no clinically recognizable cardiac abnormality. The showed left bundle branch block contour 10 17 patients, right...
Abstract Background and Objectives: To identify if any process steps used in plasma fractionation may have a capability of removing agents human transmissible spongiform encephalopathy (TSE). Materials Methods: Sixteen were investigated separately by adding preparation hamster adapted scrapie 263K to the starting material at each step determining distribution into resultant fractions protease‐K‐resistant (abnormal) prion protein Western blot analysis. Results: A number operations found...
To identify if any process steps used in plasma fractionation may have a capability of removing agents human transmissible spongiform encephalopathy (TSE).Sixteen were investigated separately by adding preparation hamster adapted scrapie 263K to the starting material at each step and determining distribution into resultant fractions protease-K-resistant (abnormal) prion protein Western blot analysis.A number operations found remove abnormal limit detection assay. These cold ethanol...
Abstract Protein solubility can be adequately represented by the classical Cohn equation for salting‐out of alcohol dehydrogenase and fumarase from clarified yeast homogenate with ammonium sulfate. However, constant β in this is a function contacting procedure employed. The kinetics continuous were similar fumarase. overall rate precipitation had variable order which was high initially, up to 3.1, but approached unity on completion precipitation. This followed partial resolution stage first...
Background —In patients with implantable cardioverter-defibrillators (ICDs). inappropriate shocks have been reported exposure to electronic article surveillance systems. The risk ICDs of walking through or lingering near systems requires further investigation. Methods and Results —We evaluated the response in ICD function 170 subjects during a 10- 15-second midgate walk-through extreme (2 minutes within 6 gate) 3 common Complete testing was done 169 subjects. During (very slow) systems, no...
Abstract Early detection of sepsis is key to ensure timely clinical intervention. Since very few end-to-end pipelines are publicly available, fair comparisons between methodologies difficult if not impossible. Progress further limited by discrepancies in the reconstruction onset time. This retrospective cohort study highlights variation performance predictive models under three subtly different interpretations from sepsis-III definition and compares this against inter-model differences. The...
Background and Objectives There is still uncertainty over how the agent of variant Creutzfeld‐Jakob disease (vCJD) would partition during manufacture plasma derivatives. In this study, a BSE‐derived was used as vCJD model to determine extent which infectivity could be removed by selected steps in intravenous immunoglobulin (IVIG). Materials Methods Murine‐passaged BSE (strain 301V), form microsomal fraction prepared from infected brain, ‘spike’ starting material three experiments. The...
Unexplained Syncope in AVID. Introduction: A prospective registry and substudy were conducted the Antiarrhythmics Versus Implantable Defibrillators (AVID) Study to clarify prognosis recurrent event rate, risk factors, impact of implantable cardioverter defibrillator (ICD) therapy patients with unexplained syncope, structural heart disease, inducible ventricular tachyarrhythmias. Methods Results: Included AVID from all participating sites who had “out hospital syncope disease EP‐inducible...
Cryoprecipitation is commonly used as the primary step in preparation of clinical factor VIII concentrates; yet recovery usually very low. Much this loss due to poor temperature control and a process continuous plasma thawing has been designed overcome this. A substantial improvement resulted, with an increase both yield purity VIII:C over 50% comparison conventional batch thaw process.
The effects of aprindine HCL on cardiac tissues were studied in anesthetized, open-chest dogs pretreated with atropine (0.8 mg/kg) and propranolol (1.0 mg/kg). Aprindine (10(-5)-10(-3) g/ml), injected into the sinus node artery, decreased spontaneous rate; when atrioventricular nodal (10(-4) g/ml) prolonged conduction time functional refractory period node. Intravenous administration (1.4, 2.8 4.2 mg/kg cumulative dose) atrial ventricular effective both tissues. results from this study...
Background and Objectives The risk of haemophiliacs contracting variant Creutzfeldt‐Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent which vCJD agent might be removed during preparation concentrate, partitioning a bovine spongiform encephalopathy (BSE)‐derived was measured over main purification step used prepare Scottish National Blood Transfusion Service high‐purity concentrate (Liberate®). Materials Methods...
Three groups of dogs were given ouabain (mean 60 mug/kg) until an accelerated ventricular escape (AVE) and repetitive response (RVR) followed cessation pacing. In a group six control dogs, the AVE RVR found to occur at stable intervals for periods least three hours. A second received various antiarrhythmic agents in attempt suppress RVR. Quinidine, diphenylhydration, lidocaine, procainamide, propranolol, successful only 0 33% trials. Potassium canrenoate, 12 mg/kg was unsuccessful dogs....
Samples taken at various stages of preparation intermediate purity factor VIII concentrate were assayed for coagulant activity (VIII:C), antigen (VIIIC:Ag) and related (VIIIR:Ag). The results used to assess mechanical loss during fractionation as these markers are relatively stable. In contrast VIII:C is sensitive both inactivation losses. major occurred the cryoprecipitation extraction step was due inactivation. Losses before after this largely VIII. Assay VIIIR:Ag in concentrates presented...