A5065253909- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- Insect symbiosis and bacterial influences
- Transgenic Plants and Applications
- Celiac Disease Research and Management
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Protein purification and stability
University of Massachusetts Chan Medical School
2020-2021
Abstract COVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for prevention or treatment to curtail mortality and morbidity. No vaccine boost mucosal immunity, as therapeutic, yet been developed SARS-CoV-2. In this study, we discover characterize cross-reactive human IgA monoclonal antibody, MAb362. MAb362 binds both SARS-CoV spike proteins competitively blocks ACE2 receptor binding, overlapping structural binding epitope. Furthermore,...
Abstract Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide. Development of vaccines against ETEC very challenging due the vast heterogeneity strains. An effective would have be multicomponent provide coverage over ten strains with genetic variabilities. There currently no vaccine licensed prevent ETEC. Nanobodies are successful new biologics in treating mucosal infectious disease as they...
COVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for prevention or treatment to curtail mortality and morbidity. No vaccine boost mucosal immunity as therapeutic yet been developed SARS-CoV-2. In this study we discover characterize cross-reactive human IgA monoclonal antibody, MAb362. MAb362 binds both SARS-CoV spike proteins competitively blocks hACE2 receptor binding, completely overlapping structural binding epitope. Furthermore,...
Disrupting transmission of Borrelia burgdorferi sensu lato complex (B. burgdorferi) from infected ticks to humans is one strategy prevent the significant morbidity Lyme disease. We have previously shown that an anti-OspA human mAb, 2217, prevents B. in animal models. Maintenance a protective plasma concentration mAb for tick season presents challenge preexposure prophylaxis strategy. Here, we describe optimization 2217 by amino acid substitutions (2217LS: M428L and N434S) Fc domain. The LS...
Mucosal surfaces of the gastrointestinal tract play an important role in immune homeostasis and defense may be compromised by enteric disorders or infection. Therapeutic intervention using monoclonal antibody (mAb) offers potential for treatment with minimal off-target effects as well possibility limited systemic exposure when administered orally. Critically, to achieve efficacy at luminal surfaces, mAb must remain stable functionally active environment. To better understand impact isotype,...
Abstract Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide. There currently no vaccine licensed prevent ETEC. Development of prophylaxis against ETEC challenging due the vast heterogeneity strains. The discovery nanobodies has emerged as a successful new biologics in treating mucosal infectious disease can recognize conserved epitopes on hypervariable pathogens. In this study, we performed large...