A5016038344- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Marine and coastal plant biology
- HIV/AIDS drug development and treatment
- Marine Biology and Ecology Research
- SARS-CoV-2 and COVID-19 Research
- Coastal wetland ecosystem dynamics
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- RNA Interference and Gene Delivery
- Genetic diversity and population structure
- Bacteriophages and microbial interactions
- Lichen and fungal ecology
- Protein purification and stability
- Animal Virus Infections Studies
- Immunodeficiency and Autoimmune Disorders
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Transgenic Plants and Applications
University of Massachusetts Chan Medical School
2012-2022
Massachusetts Academy of Math and Science
2013
Seattle University
2009
Center for Infectious Disease Research
2009
University of New Hampshire
2003-2006
Abstract COVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for prevention or treatment to curtail mortality and morbidity. No vaccine boost mucosal immunity, as therapeutic, yet been developed SARS-CoV-2. In this study, we discover characterize cross-reactive human IgA monoclonal antibody, MAb362. MAb362 binds both SARS-CoV spike proteins competitively blocks ACE2 receptor binding, overlapping structural binding epitope. Furthermore,...
Abstract Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide. Development of vaccines against ETEC very challenging due the vast heterogeneity strains. An effective would have be multicomponent provide coverage over ten strains with genetic variabilities. There currently no vaccine licensed prevent ETEC. Nanobodies are successful new biologics in treating mucosal infectious disease as they...
The high degree of morphological plasticity displayed by species the brown algal genus Fucus L. is well documented. Such variation especially pronounced for those estuarine taxa lacking holdfasts (termed ecads) that often bear little resemblance to attached from which they are derived. To better understand systematics salt marsh fucoids, we developed a suite four microsatellite‐containing loci capable distinguishing between F. vesiculosus and spiralis genetic markers were used determine...
Six species of Porphyra have commonly been recognized in the north-western Atlantic from Long Island Sound to Canadian Maritimes: P. amplissima, leucosticra, linearis, miniata, purpurea, and umbilicalis. Distinguishing them with certainty has problematic. A DNA-based system molecular identification was developed using partial sequences nuclear small subunit ribosomal RNA gene (SSU) or plastid ribulose-1,5-bisphosphate carboxylase–oxygenase large (rbcL). Multiple samples each taxon were...
Glycoprotein gp120 is a surface antigen and virulence factor of human immunodeficiency virus 1. Broadly neutralizing antibodies (bNAbs) that react to from variety HIV isolates offer hope for the development broadly effective immunogens vaccination purposes, if interactions between bNAbs can be understood. From structural perspective, particularly difficult system because its size, presence multiple flexible regions, large amount glycosylation, all which are important in gp120–bNAb...
COVID-19 caused by SARS-CoV-2 has become a global pandemic requiring the development of interventions for prevention or treatment to curtail mortality and morbidity. No vaccine boost mucosal immunity as therapeutic yet been developed SARS-CoV-2. In this study we discover characterize cross-reactive human IgA monoclonal antibody, MAb362. MAb362 binds both SARS-CoV spike proteins competitively blocks hACE2 receptor binding, completely overlapping structural binding epitope. Furthermore,...
The majority of available monoclonal antibodies (MAbs) in the current HIV vaccine field are generated from HIV-1-infected people. In contrast, preclinical immunogenicity studies have mainly focused on polyclonal antibody responses experimental animals. Although rabbits been widely used for studies, there has no report using rabbit MAbs to dissect specificity AIDS development. Here we production a panel 12 New Zealand White (NZW) that was immunized with an HIV-1 JR-FL gp120 DNA prime and...
Dwarf embedded Fucus populations in the Northwest Atlantic Ocean are restricted to upper intertidal zone sandy salt marsh environments; they lack holdfasts and from attached parental of F. spiralis or spiralis×F. vesiculosus hybrids after breakage entanglement with halophytic grasses. forms dichotomously branched, flat, have a mean overall length width 20.3 1.3 mm, respectively. Thus, longer than Irish (mean 9.3 mm) Alaskan 15.0 identified as cottonii. Reciprocal transplants different taxa...
ABSTRACT HIV-1 R5 viruses vary extensively in their capacity to infect macrophages. that confer efficient infection of macrophages are able exploit low levels CD4 for and predominate brain tissue, where a major target infection. founder transmitted were reported be non-macrophage-tropic. Here, we investigated the sensitivities macrophage-tropic non-macrophage-tropic envelopes neutralizing antibodies. We observed striking differences Env + pseudovirions soluble (sCD4) monoclonal antibodies...
Mucosal surfaces of the gastrointestinal tract play an important role in immune homeostasis and defense may be compromised by enteric disorders or infection. Therapeutic intervention using monoclonal antibody (mAb) offers potential for treatment with minimal off-target effects as well possibility limited systemic exposure when administered orally. Critically, to achieve efficacy at luminal surfaces, mAb must remain stable functionally active environment. To better understand impact isotype,...
ABSTRACT HIV-1 R5 variants exploit CCR5 as a coreceptor to infect both T cells and macrophages. viruses that are transmitted or derived from immune tissue peripheral blood mainly inefficient at mediating infection of In contrast, highly macrophage-tropic (mac-tropic) predominate in brain can be detected cerebrospinal fluid but infrequent even late disease. These mac-tropic carry envelope glycoproteins (Envs) adapted low levels CD4 on macrophages induce infection. However, it is unclear...
Anti-COVID antibody therapeutics have been developed but not widely used due to their high cost and escape of neutralization from the emerging variants. Here, we describe development VHH-IgA1.1, a nanobody IgA fusion molecule as an inhalable, affordable less invasive prophylactic therapeutic treatment against SARS-CoV-2 Omicron VHH-IgA1.1 recognizes conserved epitope spike protein Receptor Binding Domain (RBD) potently neutralizes major global variants concern (VOC) including variant its sub...
In the current study, immune responses induced by Gag DNA vaccines with different designs were evaluated in Balb/C mice. The results demonstrated that vaccine full length wild type gag gene (Wt-Gag) mainly produced antigens intracellularly and a higher level of cell-mediated (CMI) responses, as measured IFN-gamma ELISPOT, intracellular cytokine staining (ICS), cytotoxic T lymphocytes (CTL) assays against dominant CD8(+) cell epitope (AMQMLKETI). contrast, addition tissue plasminogen...
Our previous preclinical studies and a Phase I clinical trial DP6-001 have indicated that polyvalent Env formulation was able to elicit broadly reactive antibody responses including low titer neutralizing against viral isolates of subtypes A, B, C AE. In the current report, panel 62 gp120 immunogens were screened in rabbit model identify can improved binding some them be included next formulation. Only about 19% this antibodies greater than 50% viruses high throughput PhenoSense...
In this study we examined whether human immunodeficiency virus type 1 (HIV-1) is equally susceptible to neutralization by a given antibody when the epitope of introduced at different positions within viral envelope glycoprotein (Env). To end, two exogenous "epitope tags" locations three major Env regions in distinct HIV-1 isolates. We how introduction epitopes affects expression, incorporation into virions, fusogenic potential, and susceptibility neutralization. Our data indicate that even...
Broadly neutralizing, anti-HIV-1 gp120 mAbs have been isolated from infected individuals, and there is considerable interest in developing these reagents for Ab-based immunoprophylaxis treatment. As a means to identify potentially new anti-HIV Abs, we exploited humanized NOD-
Immunoglobulin A (IgA) antibodies are critical to mucosal protection, specifically dimeric IgA (dIgA) and secretory (sIgA), which rely on the J chain polymerize. There is an absence of monoclonal that can bind polymeric without need denature molecule. We generated a panel highly specific mouse anti-J react with both intact denatured nonhuman primate dIgA human sIgA IgA1 IgA2 subclass. expanded use this antibody for quantification using biolayer interferometry or enzyme-linked immunosorbent...
Background HIV-1 R5 viruses vary extensively in their capacity to infect macrophages. that confer efficient infection of macrophages are able exploit low levels CD4 for and they predominate brain tissue where a major target infection. variants transmitted generally non-macrophage-tropic. Here, we investigated the sensitivity macrophage-tropic non-macrophage-tropic envelopes neutralizing antibodies.
Background The identification of phenotypic features the HIV-1 envelope glycoprotein that correlate with neutralization breadth is an important goal HIV vaccine research. Recently we compared immunogenic potential two gp120s differing in their ability to utilize CD4; B33 (highly macrophage topic) and LN40 (non-macrophage tropic). Using a DNA prime protein boost regimen New Zealand White Rabbits, LN40-primed sera displayed enhanced B33-primed group, differences immunogenicity between groups...
Abstract Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide. There currently no vaccine licensed prevent ETEC. Development of prophylaxis against ETEC challenging due the vast heterogeneity strains. The discovery nanobodies has emerged as a successful new biologics in treating mucosal infectious disease can recognize conserved epitopes on hypervariable pathogens. In this study, we performed large...