A5089989887- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Lipid Membrane Structure and Behavior
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Ion channel regulation and function
- Antibiotics Pharmacokinetics and Efficacy
- Mechanical Systems and Engineering
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant and Biological Electrophysiology Studies
- Soil Mechanics and Vehicle Dynamics
- Polyamine Metabolism and Applications
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Mechanical Engineering and Vibrations Research
- Vibrio bacteria research studies
- Urinary Tract Infections Management
- Pneumocystis jirovecii pneumonia detection and treatment
- Infections and bacterial resistance
- Antimicrobial Peptides and Activities
- Molecular Sensors and Ion Detection
- Pediatric Hepatobiliary Diseases and Treatments
- DNA and Nucleic Acid Chemistry
- Antibiotic Use and Resistance
Entasis Therapeutics (United States)
2017-2023
Innoviva (United States)
2023
Vertex Pharmaceuticals (United States)
2015-2018
Howard Hughes Medical Institute
2002-2007
Tufts University
2005
University of Houston
1997-2003
Brandeis University
2002
We characterized the role of catabolite control protein A (ccpA) in physiology and virulence Streptococcus pneumoniae. S. pneumoniae has a large percentage its genome devoted to sugar uptake metabolism, therefore, regulation these processes is likely be crucial for fitness nasopharynx may play during invasive disease. In many bacteria, carbon repression (CCR) central such regulation, influencing hierarchical utilization growth rates. CcpA major transcriptional regulator CCR several...
OmpAAb is a conserved, abundantly expressed outer membrane porin in Acinetobacter baumannii whose presumed role antibiotic permeation has not been clearly demonstrated. In this report, we use titratable heterologous expression system to express isolation and demonstrate selective passage of small molecule antibiotics through OmpAAb. ETX2514, recently discovered broad-spectrum β-lactamase inhibitor, combination with sulbactam, currently clinical testing for the treatment drug-resistant A....
The effects of four polyamines (putrescine, cadaverine, spermidine, and spermine) on the activity bacterial porins OmpC OmpF were investigated by electrophysiology. Membrane vesicles made from outer membrane Escherichia colistrains expressing only or reconstituted into liposomes probed patch clamp. channel was recorded in control solutions presence increasing concentrations a specific polyamine. In all cases, concentration- voltage-dependent inhibitory observed. They include both suppression...
Summary We characterized two sucrose‐metabolizing systems – sus and scr describe their roles in the physiology virulence of Streptococcus pneumoniae murine models carriage pneumonia. The are regulated by LacI family repressors SusR ScrR respectively. regulates an adjacent ABC transporter ( susT1 / susT2 susX ) sucrose‐6‐phosphate (S‐6‐P) hydrolase susH ). controls PTS scrT ), fructokinase scrK second S‐6‐P scrH play niche‐specific virulence. locus mutants attenuated lung, but dispensable...
ABSTRACT Acinetobacter baumannii - calcoaceticus complex (ABC) causes severe infections that are difficult to treat due pre-existing antibiotic resistance. Sulbactam-durlobactam (SUL-DUR) is a targeted β-lactam/β-lactamase inhibitor combination designed serious caused by , including multidrug- and carbapenem-resistant strains. In recent global surveillance study of 5,032 ABC clinical isolates collected from 2016 2021, less than 2% had SUL-DUR MIC values >4 µg/mL. Molecular...
A direct assay of efflux by Escherichia coli AcrAB-TolC and related multidrug pumps would have great value in discovery new Gram-negative antibiotics. The current understanding how is affected the chemical structure physical properties molecules extremely limited, derived from antibacterial data for compounds that inhibit growth wild-type E. coli. We adapted a previously described fluorescent to 96-well microplate format measured ability test compete with Nile Red (an environment-sensitive...
A general method for determining bacterial uptake of compounds independent antibacterial activity would be a valuable tool in drug discovery. LC-MS/MS assays have been described, but it has not shown whether the data can used directly to inform medicinal chemistry. We describe evaluation an assay measuring association with bacteria, using set over hundred (inhibitors NAD-dependent DNA ligase, LigA) which vitro potency and had determined. All were active against efflux-deficient strain...
The global emergence of antibiotic resistance, especially in Gram-negative bacteria, is an urgent threat to public health. Discovery novel classes antibiotics with activity against these pathogens has been impeded by a fundamental lack understanding the molecular drivers underlying small molecule uptake. Although it well-known that outer membrane porins represent main route entry for small, hydrophilic molecules across cell envelope, structure–permeation relationship porin passage yet be...
The L3 loop is an important feature of the OmpF porin structure, contributing to both channel size and electrostatic properties. Colicins A N, spermine, antibiotics that use penetrate cell, were used investigate structure–function relationships L3. Spermine was found protect efficiently cells expressing wild-type from colicin action. Among other solutes, sugars had minor effects on activity, whereas competitions between antibiotic fluxes observed. tested, cefepime appeared most efficient....
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