A5088010877- Pneumonia and Respiratory Infections
- SARS-CoV-2 detection and testing
- Bacterial Infections and Vaccines
- Biosensors and Analytical Detection
- Antibiotics Pharmacokinetics and Efficacy
- Antifungal resistance and susceptibility
- SARS-CoV-2 and COVID-19 Research
- Fungal Infections and Studies
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Helicobacter pylori-related gastroenterology studies
- Streptococcal Infections and Treatments
- Infectious Diseases and Tuberculosis
- COVID-19 Clinical Research Studies
- Hematological disorders and diagnostics
- Sinusitis and nasal conditions
- Orthopedic Infections and Treatments
- Antibiotic Use and Resistance
- Viral gastroenteritis research and epidemiology
- Gastrointestinal disorders and treatments
- Clostridium difficile and Clostridium perfringens research
- Medical Coding and Health Information
- Gastroesophageal reflux and treatments
- Toxoplasma gondii Research Studies
Hospital Pablo Tobon Uribe
2011-2024
Queensborough Community College, CUNY
2011-2023
City University of New York
2015-2023
Stockholm Environment Institute
2023
The Graduate Center, CUNY
2021-2022
Clínica CES
2014-2022
Universidad Pontificia Bolivariana
2022
New School
2021-2022
Queens College, CUNY
2021-2022
Digital Health Cooperative Research Centre
2017
Tracking SARS-CoV-2 genetic diversity is strongly indicated because diversifying selection may lead to the emergence of novel variants resistant naturally acquired or vaccine-induced immunity. To monitor New York City (NYC) for presence variants, we deep sequence most receptor binding domain coding S protein isolated from wastewater. Here report detecting increasing frequencies cryptic lineages not recognized in GISAID's EpiCoV database. These contain mutations that had been rarely observed...
Objectives. To determine the etiology of community-acquired pneumonia in ambulatory children and to compare responses treatment with azithromycin, amoxicillin-clavulanate or erythromycin estolate. Methods. Ambulatory patients were identified at Children's Medical Center Dallas, TX. Children age 6 months 16 years radiographic clinical evidence enrolled randomized receive either azithromycin suspension for 5 days a 10-day course those <5 estolate ≥5 years. Blood culture was obtained all we...
Global assessment of antimicrobial agents prescribed to infants in the neonatal intensive care unit (NICU) may inform stewardship efforts.We conducted a one-day global point prevalence study all antimicrobials provided NICU infants. Demographic, clinical, and microbiologic data were obtained including level, census, birth weight, gestational/chronologic age, diagnoses, therapy (reason for use; length therapy), program (ASP), 30-day in-hospital mortality.On July 1, 2019, 26% (580/2,265;...
Abstract Background Pneumonia is the leading cause of mortality in pediatric population. The etiology pneumonia this population variable and changes according to age disease severity where study conducted. Our aim was determine community-acquired (CAP) children aged 1 month 17 years admitted 13 Colombian hospitals. Methods Prospective cohort study. Hospitalized with radiologically confirmed CAP ≤ 15 days symptoms were included followed together a control group. Induced sputum (IS) submitted...
Wastewater-based epidemiology (WBE) is an effective way of tracking the appearance and spread SARS-COV-2 lineages through communities. Beginning in early 2021, we implemented a targeted approach to amplify sequence receptor binding domain (RBD) characterize viral present sewersheds. Over course reproducibly detected multiple RBD that have never been observed patient samples 9 sewersheds located 3 states USA. These cryptic contained between 4 24 amino acid substitutions were intermittently...
SARS-CoV-2 surveillance of viral populations in wastewater samples is recognized as a useful tool for monitoring epidemic waves and boosting health preparedness. Next generation sequencing RNA isolated from convenient cost-effective strategy to understand the molecular epidemiology provide insights on population dynamics variants at community level. However, low- middle-income countries, groups have performed data has not been extensively shared scientific community. Here we report results...
SARS-CoV-2 viral loads in New York City were significantly correlated with clinical case rates corresponding sewersheds.
Forty-seven patients with active paracoccidioidomycosis were treated itraconazole. The majority of the (45) had chronic adult form disease while remaining exhibited juvenile form. All received itraconazole, 100 mg day-1, exception two for whom clinical condition necessitated a larger dose. mean duration therapy was 6 months, range 3-24 months. Clinically, skin and mucous membrane lesions as well abdominal pain, auscultatory alterations dysphonia improved markedly during treatment concomitant...
Background : We assessed the efficacy, compliance, and tolerability of twice‐a‐day triple therapy, amoxycillin, omeprazole, clarithromycin, for Helicobacter pylori studied effect treatment duration (7, 10 or 14 days) on these factors. Methods One‐hundred fifty subjects with H. infection documented by 13 C‐urea breath test were randomly assigned to a 7, 14‐day course amoxycillin 1 g b.d., omeprazole 20 mg b.d. clarithromycin 500 Subjects returned at end therapy pill count assessment...
Background Cerebrospinal fluid (CSF) values in the noninfected neonate are not well-delineated. Studies analyzing these inconsistent criteria used to define population. The purpose of our study was examine CSF neonates first 30 days life whom infection more thoroughly excluded than previous reports. Stringent inclusion defined population, and recently available polymerase chain reaction (PCR) for enteroviruses addition cultures help exclude viral disease. Results were also stratified by age...
ABSTRACT With the emergence of β-lactam antibiotic resistance among strains Streptococcus pneumoniae , vancomycin has assumed an important role in treatment bacterial meningitis. Using rabbit meningitis model, we evaluated pharmacokinetics and pharmacodynamics this setting. Animals were given 80 mg/kg body weight daily two or four divided doses to determine penetration activity cerebrospinal fluid (CSF); each regimen was administered with without dexamethasone. Mean peak (2 h) concentrations...
Multidrug-resistant Gram-negative infections represent a growing problem and serious global threat. Data in children are scarce. Klebsiella pneumoniae carbapenemases (KPC) the most common mechanism of resistance this organism has developed. We report clinical characteristics outcomes from cohort infected or colonized with carbapenem-resistant K. (CRKp) at tertiary care center Medellín, Colombia.We performed retrospective chart review all pediatric cases whom CRKp isolates were obtained 2008...
Abstract Tracking SARS-CoV-2 genetic diversity is strongly indicated because diversifying selection may lead to the emergence of novel variants resistant naturally acquired or vaccine-induced immunity. To monitor New York City (NYC) for presence variants, we amplified regions Spike protein gene from RNA all 14 NYC wastewater treatment plants (WWTPs) and ascertained lineages these samples using high throughput sequencing. Here report detection increasing frequencies not recognized in GISAID’s...
Adequate concentrations of beta-lactam antibiotics in cerebrospinal fluid (CSF) are difficult to achieve for meningitis caused by drug-resistant Streptococcus pneumoniae. Ceftriaxone dosages 150 or 400 mg/kg body weight per day, given one two doses, was used the treatment experimental highly cephalosporin-resistant (MIC and MBC, 4 microg/ml) pneumococcal meningitis. The bacterial killing rate (delta log10 CFU milliliter hour) pharmacokinetic indices, including percentage time antibiotic...
We have expressed catalytically active Toxoplasma gondii dihydrofolate−thymidylate synthase (DHFR-TS) and the individual TS DHFR domains in Escherichia coli using T7 promoter of pET-15b. DHFR-TS constituted approximately 10% total soluble cell protein was purified methotrexate-Sepharose chromatography to yield 10 mg homogeneous per liter culture. The domain recovered as insoluble inclusion bodies which could be unfolded refolded recover soluble, enzyme. overexpressed a by growing cells at 24...
The following protocol describes our workflow for processing wastewater with the goal of detecting genetic signal SARS-CoV-2. steps include pasteurization, virus concentration, RNA extraction, and quantification by RT-qPCR. We auxiliary that provide new users tools strategies will help troubleshoot key in process. This is one safest, cheapest, most reproducible approaches detection SARS-CoV-2 wastewater. Owing to a pasteurization step, it safe use BSL2 facility. In addition making personnel...
Introduction Invasive Pneumococcal Disease (IPD) causes significant morbidity and mortality in children under 5 y. Colombia introduced PCV10 vaccination 2012, the Neumocolombia network has been monitoring IPD pediatric patients since 2008. Materials methods This study is a secondary analysis of prospective cohort involving with admitted to 17 hospitals Colombia, from January 1st, 2017, December 31st, 2022. We present data on serotypes (Spn), clinical characteristics, resistance patterns....
Interleukin-10 (IL-10) is a cytokine with antiinflammatory effects. In rabbit model of meningitis, IL-10 was given intracisternally or intravenously to evaluate the impact on inflammation induced by lipooligosaccharide (LOS), Haemophilus influenzae type b (Hib), Listeria monocytogenes. Intracisternal in concentrations >1 microg significantly reduced tumor necrosis factor-alpha (TNF-alpha) and lactate values cerebrospinal fluid (CSF). Intravenous (1 mg/kg) two doses after intracisternal LOS...
Fungal infections have become an important cause of morbidity and mortality in hospitalized children due to many complicating underlying conditions. We present the case a newborn infant with fungemia Kodamaea ohmeri who had good outcome infection after using combination antifungal treatment central venous catheter removal.
CP-99,219 is a new fluoroquinolone that has excellent activity against gram-positive organisms including penicillin- and cephalosporin-resistant Streptococcus pneumoniae strains. In our well-established rabbit model of meningitis, we conducted experiments to determine the concentrations in cerebrospinal fluid (CSF) after intravenous administration its ability eradicate two penicillin-resistant pneumococcal isolates. The peak trough CSF were from 19 25% simultaneously obtained serum...