A5014386804- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neural dynamics and brain function
- Cancer Cells and Metastasis
- Skin and Cellular Biology Research
- Machine Learning in Materials Science
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Immunotherapy and Immune Responses
- Olfactory and Sensory Function Studies
- Cutaneous Melanoma Detection and Management
- Electron and X-Ray Spectroscopy Techniques
- Advanced Memory and Neural Computing
- Bee Products Chemical Analysis
- Interconnection Networks and Systems
- Cellular Mechanics and Interactions
- Nonmelanoma Skin Cancer Studies
- Biochemical Analysis and Sensing Techniques
- Digital Holography and Microscopy
- Manufacturing Process and Optimization
- Spectroscopy and Chemometric Analyses
- Microtubule and mitosis dynamics
- Advanced X-ray Imaging Techniques
- Functional Brain Connectivity Studies
The Francis Crick Institute
2024
Max Planck Institute for Brain Research
2015-2022
German Cancer Research Center
2015-2017
Heidelberg University
2015-2017
Max Planck Institute for Medical Research
2012
The dense circuit structure of mammalian cerebral cortex is still unknown. With developments in three-dimensional electron microscopy, the imaging sizable volumes neuropil has become possible, but reconstruction connectomes limiting step. We reconstructed a volume ~500,000 cubic micrometers from layer 4 mouse barrel cortex, ~300 times larger than previous reconstructions cortex. connectomic data allowed extraction inhibitory and excitatory neuron subtypes that were not predictable geometric...
Nerve tissue contains a high density of chemical synapses, about 1 per µm3 in the mammalian cerebral cortex. Thus, even for small blocks nerve tissue, dense connectomic mapping requires identification millions to billions synapses. While focus data analysis has been on neurite reconstruction, synapse detection becomes limiting when datasets grow size and is required. Here, we report SynEM, method automated synapses from conventionally en-bloc stained 3D electron microscopy image stacks. The...
Understanding the function of biological tissues requires a coordinated study physiology and structure, exploring volumes that contain complete functional units at detail resolves relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing region where was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded imaged with synchrotron X-ray computed tomography propagation-based phase contrast (SXRT)....
Three-dimensional in vitro skin and cancer models help to dissect epidermal-dermal tumor-stroma interactions. In the model presented here, normal human dermal fibroblasts isolated from adult self-assembled into equivalents with their specific fibroblast-derived matrix (fdmDE) over 4 weeks. The fdmDE represented a complex extracellular that was stabilized by its own heterogeneous collagen fiber meshwork, largely resembling vivo architecture. Complemented epidermal keratinocytes, equivalent...
ABSTRACT The dense circuit structure of the mammalian cerebral cortex is still unknown. With developments in 3-dimensional (3D) electron microscopy, imaging sizeable volumes neuropil has become possible, but reconstruction connectomes from such image data limiting step. Here, we report a volume about 500,000 μm 3 layer 4 mouse barrel cortex, 300 times larger than previous reconstructions cortex. Using novel technique, FocusEM, were able to reconstruct total 0.9 meters dendrites and 1.8 axons...
At present, tumor diagnostic imaging is commonly based on hematoxylin and eosin or immunohistochemical staining of biopsies, which requires tissue excision, fixation, with exogenous marker molecules. Here, we report label-free using confocal spontaneous Raman scattering microspectroscopy, exploits the intrinsic vibrational contrast endogenous biomolecular species. We present a chemically specific quantitative approach to monitoring normal human skin cells (keratinocytes fibroblasts) as well...
Despite decades of skin research, regulation proliferation and homeostasis in human epidermis is still insufficiently understood. To address the role mitoses tissue regulation, we utilized long-term equivalents systematically assessed during early epidermal development regeneration. We now demonstrate four different orientations: (1) horizontal, i.e., parallel to basement membrane (BM) suggestive symmetric divisions; (2) oblique with an angle 45°-70°; or (3) perpendicular, asymmetric...
Abstract Attributing in vivo neurophysiology to the brains’ ultrastructure requires a large field of view containing contextual anatomy. Electron microscopy (EM) is gold standard technique identify ultrastructure, yet acquiring volumes full mammalian neural circuits challenging and time consuming using EM. Here, we show that synchrotron X-ray computed tomography (SXRT) provides rapid imaging EM-prepared tissue several cubic millimetres. Resolution was sufficient for distinguishing cell...
ABSTRACT Nerve tissue contains a high density of chemical synapses, about 1 per µm 3 in the mammalian cerebral cortex. Thus, even for small blocks nerve tissue, dense connectomic mapping requires identification millions to billions which 80-90% are excitatory synapses. While focus data analysis has been on neurite reconstruction, synapse detection becomes limiting when datasets grow size and is required. Here, we report SynEM, method automated synapses from conventionally en-bloc stained 3D...
Biological soft tissues are functional agglomerates of cells. They constitute the microenvironment where intercellular communication occurs. In turn, their woven structure underlies mechanical properties that contribute to roles in context organs and organisms contain them. Therefore, determining density spatial distribution cells within tissue offers key information for understanding its physiological state. X-ray holographic nanotomography is a non-destructive imaging technique capable...
Abstract 3D in vitro skin models are valuable research tools to investigate normal keratinocyte as well carcinoma cell interaction with dermal fibroblasts. Currently, the lack either a structured vital human extracellular matrix or long-term stability and longevity. Therefore, we aimed at new model for epidermis tumorigenic stages of cancer. This is based on equivalents containing highly cell-derived (CDM) produced by First, show that CDM equivalent supports high structural integrity...
Abstract Adverse effects of melanoma therapy by the BRAFV600E-targeting small-molecule inhibitor Vemurafenib lead to development skin keratoacanthomas (KAs) and squamous cell carcinomas (SCCs) in a significant number patients. Since KAs SCCs are observed within few weeks after onset therapy, we hypothesize that treatment is directly involved progression preexisting oncogenic keratinocyte lesions. Using monolayer cultures normal human dermal epidermal cells as well 3D organotypic co-cultures...