A5004031189- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Foot and Ankle Surgery
- Urological Disorders and Treatments
- Glioma Diagnosis and Treatment
- Orthopedic Surgery and Rehabilitation
- Neurological disorders and treatments
- Congenital Diaphragmatic Hernia Studies
- Advanced Memory and Neural Computing
- Functional Brain Connectivity Studies
- Cerebrospinal fluid and hydrocephalus
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Elbow and Forearm Trauma Treatment
- Urinary and Genital Oncology Studies
Medanta The Medicity
2024
Max Planck Institute for Brain Research
2018-2024
Radboud University Nijmegen
2022
The dense circuit structure of mammalian cerebral cortex is still unknown. With developments in three-dimensional electron microscopy, the imaging sizable volumes neuropil has become possible, but reconstruction connectomes limiting step. We reconstructed a volume ~500,000 cubic micrometers from layer 4 mouse barrel cortex, ~300 times larger than previous reconstructions cortex. connectomic data allowed extraction inhibitory and excitatory neuron subtypes that were not predictable geometric...
The human cerebral cortex houses 1000 times more neurons than that of the a mouse, but possible differences in synaptic circuits between these species are still poorly understood. We used three-dimensional electron microscopy macaque, and cortical samples to study their cell type composition circuit architecture. 2.5-fold increase interneurons humans compared with mice was compensated by change axonal connection probabilities therefore did not yield commensurate inhibitory-versus-excitatory...
ABSTRACT The cerebral cortex of mammals has long been proposed to comprise unit-modules, so-called cortical columns. detailed synaptic-level circuitry such a neuronal network about 10 4 neurons is still unknown. Here, using 3-dimensional electron microscopy, AI-based image processing and automated proofreading, we report the connectomic reconstruction defined column in mouse barrel cortex. appears as structural feature connectome, without need for geometrical or morphological landmarks. We...
Sensory signals are transmitted via the thalamus primarily to layer 4 (L4) of primary sensory cortices. While information about average neuronal connectivity in L4 is available, its detailed higher-order circuit structure not known. Here, we used three-dimensional electron microscopy for a connectomic analysis thalamus-driven inhibitory network L4. We find that thalamic input drives subset interneurons with high specificity, which turn target excitatory neurons subtype specificity. These...
ABSTRACT The dense circuit structure of the mammalian cerebral cortex is still unknown. With developments in 3-dimensional (3D) electron microscopy, imaging sizeable volumes neuropil has become possible, but reconstruction connectomes from such image data limiting step. Here, we report a volume about 500,000 μm 3 layer 4 mouse barrel cortex, 300 times larger than previous reconstructions cortex. Using novel technique, FocusEM, were able to reconstruct total 0.9 meters dendrites and 1.8 axons...
ABSTRACT In mammals, sensory signals are transmitted via the thalamus primarily to layer 4 of primary cortices. While information about average neuronal connectivity in this is available, detailed and higher-order circuit structure not known. Here, we used 3-dimensional electron microscopy for a connectomic analysis thalamus-driven inhibitory network barrel. We find that thalamic input drives subset interneurons with high specificity. These turn target spiny stellate star pyramidal...