A5065464798- Enzyme Structure and Function
- Microfluidic and Capillary Electrophoresis Applications
- RNA and protein synthesis mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Electron Microscopy Techniques and Applications
- Nanofabrication and Lithography Techniques
- Protein Structure and Dynamics
- Multiple Sclerosis Research Studies
- Mass Spectrometry Techniques and Applications
- Surface Modification and Superhydrophobicity
- Polymer Surface Interaction Studies
- Endoplasmic Reticulum Stress and Disease
- Magnetic and Electromagnetic Effects
- Advanced X-ray Imaging Techniques
- Nanopore and Nanochannel Transport Studies
- Crystallization and Solubility Studies
- Peripheral Neuropathies and Disorders
- Biochemical and Molecular Research
Cornell University
2023
Ithaca College
2023
University of Massachusetts Amherst
2016-2021
Universidad Nacional Autónoma de México
2019
Institute of Physical Chemistry
2019
Bulgarian Academy of Sciences
2019
Tohoku University
2019
Amherst College
2016
The advent of biocatalysts designed computationally and optimized by laboratory evolution provides an opportunity to explore molecular strategies for augmenting catalytic function. Applying a suite nuclear magnetic resonance, crystallography, stopped-flow techniques enzyme elementary proton transfer reaction, we show how directed gradually altered the conformational ensemble protein scaffold populate narrow, highly active accelerate this transformation nearly nine orders magnitude. Mutations...
Microfluidic strategies to enable the growth and subsequent serial crystallographic analysis of micro-crystals have potential facilitate both structural characterization dynamic studies protein targets that been resistant single-crystal strategies. However, adapting microfluidic crystallization platforms for micro-crystallography requires a dramatic decrease in overall device thickness. We report robust strategy straightforward incorporation single-layer graphene into ultra-thin devices....
Capturing protein structural dynamics in real-time has tremendous potential elucidating biological functions and providing information for structure-based drug design. While time-resolved structure determination long been considered inaccessible a vast majority of targets, serial methods crystallography have remarkable facilitating such analyses. Here, we review the impact microfluidic technologies on crystal growth X-ray diffraction analysis. In particular, focus applications microfluidics...
We report the fabrication, properties, and bacteria-resistance of polyelectrolyte complex (PEC) coatings free-standing films. Poly(4-styrenesulfonic acid), poly(diallyldimethyl-ammonium chloride), salt were spin-coated into PEC After thermal annealing in a humid environment, highly transparent, mechanically strong, chemically robust films formed. Notably, we demonstrate that significantly reduce attachment Escherichia coli K12 without killing micro-organisms. suggest forming...
RNA macromolecules, like proteins, fold to assume shapes that are intimately connected their broadly recognized biological functions; however, because of high charge and dynamic nature, structures far more challenging determine. We introduce an approach exploits the brilliance x-ray free-electron laser sources reveal formation ready identification angstrom-scale features in structured unstructured RNAs. Previously unrecognized structural signatures secondary tertiary identified through...
Here, we describe a novel microfluidic platform for use in electrocrystallization experiments. The device incorporates ultra-thin graphene-based films as electrodes and X-ray transparent windows to enable situ diffraction analysis. Furthermore, large-area graphene serve gas barrier, creating stable sample environment over time. We characterize different methods fabricating electrodes, validate the electrical capabilities of our through methyl viologen, redox-sensitive dye. Proof-of-concept...
A novel capillary-based microfluidic strategy to accelerate the process of small-molecule-compound screening by room-temperature X-ray crystallography using protein crystals is reported. The ultra-thin devices are composed a UV-curable polymer, patterned cleanroom photolithography, and have nine capillary channels per chip. chip was designed for ease sample manipulation, stability minimal background. 3D-printed frames cassettes conforming SBS standards used house chips, providing additional...
RNA macromolecules, like proteins, fold to assume shapes that are intimately connected their broadly recognized biological functions; however, because of high charge and dynamic nature, structures far more challenging determine. We introduce an approach exploits the brilliance x-ray free electron laser sources reveal formation ready identification Å scale features in structured unstructured RNAs. New structural signatures secondary tertiary identified through wide angle solution scattering...