A5066785148- Advanced Fluorescence Microscopy Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Laser Material Processing Techniques
- Barrier Structure and Function Studies
- Photoacoustic and Ultrasonic Imaging
- Neurological Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Nonlinear Optical Materials Studies
- Optical Imaging and Spectroscopy Techniques
- Acute Ischemic Stroke Management
- Photoreceptor and optogenetics research
- Ocular and Laser Science Research
- Neurological Disorders and Treatments
- Cell Image Analysis Techniques
- Zebrafish Biomedical Research Applications
- Optical Coherence Tomography Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced Fiber Laser Technologies
- Laser-Matter Interactions and Applications
- Intracerebral and Subarachnoid Hemorrhage Research
- Neuroscience and Neuropharmacology Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- 3D Printing in Biomedical Research
- Laser-induced spectroscopy and plasma
Cornell University
2015-2024
Ithaca College
2023
Kobe University
2021
Saint Meinrad Seminary and School of Theology
2016
Harvard University
1997-2011
Goethe University Frankfurt
2011
Massachusetts General Hospital
2011
University of California, San Diego
2003-2007
Harvard University Press
1998-2006
Center for Theoretical Biological Physics
2005-2006
Using tightly focused femtosecond laser pulses of just 5 nJ, we produce optical breakdown and structural change in bulk transparent materials demonstrate micromachining by use unamplified lasers. We present measurements the threshold for Corning 0211 glass as well a study morphology structures produced single multiple pulses. At high repetition rate, dominated cumulative heating material successive this effect, write single-mode waveguides inside glass, using only oscillator.
Laser-induced breakdown and damage to transparent materials has remained an active area of research for four decades. In this paper we review the basic mechanisms that lead laser-induced present a summary some open questions in field. We method measuring threshold intensity required produce bulk, as opposed on surface, material. Using technique, measure material band-gap laser-wavelength dependence bulk using femtosecond laser pulses. Based these thresholds, determine relative role different...
We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that 1280-nm through in vivo and ex TPM of fluorescently-labeled blood vessels mouse brain. high contrast at approximately twice as excitation. An 1 mm can be adult brains 1280 nm 1-nJ pulse energy sample surface. Blood flow speed measurements a 900 mum are performed.
A highly interconnected network of arterioles overlies mammalian cortex to route blood the cortical mantle. Here we test if this angioarchitecture can ensure that supply is redistributed after vascular occlusion. We use rodent parietal as a model system and image flow red cells in individual microvessels. Changes are quantified response photothrombotic occlusions pial well physical middle cerebral artery (MCA), primary source network. observe perfusion rapidly reestablished at first branch...
Inhibiting endothelial cell contractility reverses the deleterious effects of age-related matrix stiffening on normal function, which could help prevent development atherosclerosis.
Penetrating arterioles bridge the mesh of communicating on surface cortex with subsurface microvascular bed that feeds underlying neural tissue. We tested conjecture penetrating arterioles, which are positioned to regulate delivery blood, loci severe ischemia in event occlusion. Focal photothrombosis was used occlude single rat parietal cortex, and resultant changes flow red blood cells were measured two-photon laser-scanning microscopy individual microvessels surround observed average...
Metastasis through the bloodstream contributes to poor prognosis in many types of cancer. Mounting evidence implicates selectin-based adhesive interactions between cancer cells and blood vessel wall as facilitating this process, a manner similar leukocyte trafficking during inflammation. Here, we describe unique approach target kill colon prostate that causes circulating leukocytes present cancer-specific TNF-related apoptosis inducing ligand (TRAIL) on their surface along with E-selectin...
Using time-resolved imaging and scattering techniques, we directly indirectly monitor the breakdown dynamics induced in water by femtosecond laser pulses over eight orders of magnitude time. We resolve, for first time, picosecond plasma observe a 20 ps delay before laser-produced expands. attribute this to electron-ion energy transfer
Subtle alterations in cerebral blood flow can impact the health and function of brain cells are linked to cognitive decline dementia. To understand hemodynamics three-dimensional vascular network cortex, we applied two-photon excited fluorescence microscopy measure motion red (RBCs) individual microvessels throughout hierarchy anesthetized mice. resolve heartbeat- respiration-dependent dynamics, simultaneously recorded electrocardiogram respiratory waveform. We found that centerline RBC...
Abstract The ApoE4 allele is associated with increased risk of small vessel disease, which a cause vascular cognitive impairment. Here, we report that mice targeted replacement (TR) the ApoE gene human have reduced neocortical cerebral blood flow compared to ApoE3-TR mice, an effect due density rather than slowing microvascular red cell flow. Furthermore, homeostatic mechanisms matching local delivery brain activity are impaired in ApoE4-TR mice. In model hypoperfusion, these cerebrovascular...
We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across 8 mm by 10 field.The lateral is approximately 1 µm and maximum scan speed 5 mm/ms.The pathway employs large diameter compound lenses to minimize aberrations performs near theoretical limits.We demonstrate special utility recording resting-state vasomotion both hemispheres brain through transcranial window imaging histological sections without need stitch.
Epileptic events initiate a large focal increase in metabolism and cerebral blood flow (CBF) to the ictal focus. In contrast, decreases CBF have been demonstrated surrounding focus, etiology of which is unknown (i.e., arising either from active shunting or passive steal). The relationship between these neuronal activity are also unknown. We investigated neurovascular neurometabolic coupling surround using optical imaging light scattering volume, autofluorescence flavoprotein (AFI), direct...