A5006966763- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Radical Photochemical Reactions
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Melamine detection and toxicity
- Neural dynamics and brain function
- Nuclear Receptors and Signaling
- Respiratory viral infections research
- Advanced Sensor and Energy Harvesting Materials
- T-cell and B-cell Immunology
- Ferroptosis and cancer prognosis
- bioluminescence and chemiluminescence research
- Neurogenesis and neuroplasticity mechanisms
- vaccines and immunoinformatics approaches
- Crystallization and Solubility Studies
- Blood properties and coagulation
Cornell University
2019-2025
Saint Meinrad Seminary and School of Theology
2025
University of Tsukuba
2023-2025
Central Hospital of Putuo District
2023
Harvard University
2017
Nanjing Normal University
2014-2015
John Radcliffe Hospital
2015
University of Oxford
2015
Photochemistry Center
2014
Analysis and Testing Centre
2014
Abstract Zwitterionic hydrogels have received great attention due to their excellent nonfouling and biocompatible properties, but they suffer from weak mechanical strength in the saline environments important for biomedical engineering applications “anti‐polyelectrolyte” effect. Conventional strategies introduce hydrophobic or non‐zwitterionic components increase compromise properties. Here, a highly effective strategy is reported achieve both high properties by constructing pure...
Secondary lymphoid organs (SLOs) are an important target for mRNA delivery in various applications. While the current method relies on drainage of nanoparticles to lymph nodes by intramuscular (IM) or subcutaneous (SC) injections, efficient carrier SLOs-targeting systemic administration (IV) is highly desirable but yet be available. In this study, we developed using phosphatidylserine (PS), a well-known signaling molecule that promotes endocytic activity phagocytes and cellular entry...
1300 nm three-photon calcium imaging has emerged as a useful technique to allow in deep brain regions. Application large-scale neural activity entails careful balance between recording fidelity and perturbation the sample. We calculated experimentally verified excitation pulse energy achieve minimum photon count required for detection of transients GCaMP6s-expressing neurons 920 two-photon 1320 excitation. By considering combined effects in-focus signal attenuation out-of-focus background...
Although recombinant adeno-associated viruses (AAVs) are considered low immunogenic and safe for gene delivery, the immunogenicity of capsids still represents a major obstacle to readministration AAV vectors. Here, we design an immunosuppressive zwitterionic phosphoserine (PS)-containing polypeptide induce AAV-specific immune tolerance eradicate immunological response. AAVs modified with PS maintain their transduction activity tissue tropism but suppress induction antibodies. In hemophilia A...
Extracellular vesicles (EVs) are preeminent carriers of biomarkers and have become the subject intense biomedical research for medical diagnostics using biosensors. To create effective EV-based immunoassays, it is imperative to develop surface chemistry approaches with optimal EV detection targeting transmembrane protein that not affected by cell-to-cell variability. Here, we developed a series immunoassays EVs derived from mouse monocyte cells plasmon resonance (SPR) We chemically...
<title>Abstract</title> Dendritic cells (DCs) can process patient-derived antigens to generate extracellular vesicles (DEVs) vaccines, which be personalized and rapidly adapted the evolving antigenic profile in chronic diseases. These DEVs present stimulate T cell responses, but their clinical efficacy is limited by poorly understood mechanisms. This study reveals how DCs incorporate ancient viral genes produce endogenous virus-like (VLVs) that enable long-range communication. Overexpressing...
The mononuclear phagocytic system is recognized as a major scavenger of mRNA-lipid nanoparticles (LNPs), clearing and redirecting these particles away from their intended targets thus diminishing delivery efficacy. Understanding the mechanism by which mRNA-LNPs interact with phagocytes how this interaction affects mRNA transfection critical to enhancing mRNA. In study, we temporarily depleted both circulating resident macrophages (MF) evaluated efficiency biodistribution mRNA-LNPs. We first...
Extracellular vesicles (EVs) are emerging as key diagnostic biomarkers due to their widespread presence in body fluids and the proteins on surfaces, which reflect identity condition of parent cells. Research has focused detecting EVs with biosensors that target individual transmembrane (TMPs) like tetraspanins. However, TMP heterogeneity formation tetraspanin-enriched microdomains (TEMs), cotargeting multiple TMPs is a promising strategy for enhancing EV detection. In this work, we introduce...
B7-H3, a recently identified B7 family member, has different isoforms in human and mouse. Mouse B7-H3 gene only one isoform (2IgB7-H3) with two Ig-like domains, whereas (2IgB7-H3 4IgB7-H3). In this study systematic genomic survey across various species from teleost fishes to mammals revealed that 4IgB7-H3 also appeared pigs, guinea cows, dogs, African elephants, pandas, megabats higher primate animals, which resulted tandem exon duplication. Further sequence analysis indicated duplication...
A general sub-stoichimetric hydrazine hydrate-mediated aerobic oxidative <italic>ipso</italic>-hydroxylation of aryl/heteroaryl boronic acids to phenols.
Sentinel lymph node (SLN) is the first regional where tumor cells metastasize, and its identification treatment are of great significance for prevention metastasis. However, current clinical modalities SLN still far from satisfactory owing to their high cost, invasiveness low accuracy. We aim design a novel nanomedicine system imaging with efficacy.We designed prepared hollow mesoporous carbon spheres (HMCS) loaded chemotherapeutic drug doxorubicin (DOX), which then modified polyvinyl...
Extracellular vesicles (EVs) are a population of vesicular bodies originating from cells, and EVs have been proven to the potential deliver different cargos, such as RNAs. However, conventional methods not able encapsulate long RNAs into efficiently or may compromise integrity EVs. In this study, we devised strategy circRNAs (>1000 nt) by harnessing sorting mechanisms cells. This utilizes inherent richness circular in genetic engineering method increase cytoplasmic concentration target...
A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result.
As some of the most widely utilised intercellular signalling molecules, transforming growth factor β (TGFβ) superfamily members play critical roles in normal development and become disrupted human disease. Establishing appropriate levels TGFβ involves positive negative feedback, which are coupled driven by same signal transduction components (R-Smad transcription complexes), but whether how regulation two can be distinguished unknown. Genome-wide comparison published ChIP-seq datasets...
Abstract 1300-nm three-photon calcium imaging has emerged as a useful technique to allow in deep brain regions. Application large-scale neural activity entails careful balance between recording fidelity and tissue heating. We calculated experimentally verified the excitation pulse energy achieve minimum photon count required for detection of transients GCaMP6s-expressing neurons 920-nm two-photon 1320-nm excitation, respectively. Brain heating by continuous was simulated with Monte Carlo...
Activity-regulated cytoskeleton-associated protein (Arc) forms virus-like capsids for mRNA transport between neurons. Unlike HIV-1 Group-specific Antigen (Gag), which uses its Nucleocapsid (NC) domain to bind genomic mRNA, mammalian Arc lacks the NC domain, and their direct binding interactions remain underexplored. This study examined rat Arc's 5' UTR (A5U), (H5U), GFP mRNAs, revealing weak with no significant preference. Adding rArc's C-terminus significantly improved H5U, while also...
Abstract Systemic delivery of mRNAs into disease neurons is first limited by the blood-brain-barrier (BBB). Leukocyte-derived extracellular vesicles (EVs) can cross BBB at inflammatory sites, emerging as promising carriers to target brain. However, efficient mRNA loading EVs and their uptake remain challenges. Here we incorporated inside endogenous retrovirus-like Arc protein capsids, stabilized 5’UTR RNA elements, effectively load deliver mRNAs. Produced from self-derived leukocytes,...
Herein, a rapid, facile, and colourimetric sensor for the detection of cyromazine in raw milk is reported using an aptamer based on gold nanoparticles (AuNPs). A sequence-specific called Tcyr1 designed to absorb surface AuNPs electrostatically interacts with poly(diallyldimethylammonium chloride) (PDDA), which prevents from aggregating. It can also self-assemble form G-quadruplex-CYR complex cyromazine. Because its specificity stability, introduction would influence protection thus following...