A5018953105- Advanced Fluorescence Microscopy Techniques
- Reproductive Biology and Fertility
- Advanced Electron Microscopy Techniques and Applications
- Photonic and Optical Devices
- Near-Field Optical Microscopy
- Semiconductor Quantum Structures and Devices
- Infrared Target Detection Methodologies
- Optical Coherence Tomography Applications
- Semiconductor Lasers and Optical Devices
- Photoacoustic and Ultrasonic Imaging
- CCD and CMOS Imaging Sensors
- Animal Genetics and Reproduction
- Digital Holography and Microscopy
- Luminescence Properties of Advanced Materials
- Photosynthetic Processes and Mechanisms
- Diamond and Carbon-based Materials Research
- Receptor Mechanisms and Signaling
- Advanced Optical Imaging Technologies
- Ion-surface interactions and analysis
- Integrated Circuits and Semiconductor Failure Analysis
- Plasmonic and Surface Plasmon Research
- Advanced Optical Sensing Technologies
- Cell Image Analysis Techniques
- Ovarian function and disorders
- Luminescence and Fluorescent Materials
Institute of Physics
2023-2024
Czech Academy of Sciences, Institute of Physics
2024
Chinese Academy of Sciences
2024
Cornell University
1994-2023
Peking University
2013-2022
Northwestern University
2022
University of Central Florida
2012-2016
Xi'an University of Technology
2008-2015
Tsinghua University
2013
State Key Laboratory of Low-Dimensional Quantum Physics
2013
Stimulated emission depletion (STED) microscopy has become a powerful imaging and localized excitation method beating the diffraction barrier for improved lateral spatial resolution in cellular imaging, lithography, etc. Due to specimen-induced aberrations scattering distortion, it been great challenge STED maintain consistent deeply inside specimens. Here we report on deep by using Gaussian beam hollow Bessel (GB-STED). The proposed scheme shows depth up ~155{\mu}m solid agarose sample,...
Abstract Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve regulate functionality. Conventional fluorescence microscopy can only provide ~300 nm resolution, which insufficient visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) study dynamic cristae live cells with superresolution technique. The low saturation intensity...
Fluorescence polarization microscopy (FPM) aims to detect the dipole orientation of fluorophores and resolve structural information for labeled organelles via wide-field or confocal microscopy. Conventional FPM often suffers from presence a large number molecules within diffraction-limited volume, with averaged fluorescence collected group dipoles different orientations. Here, we apply sparse deconvolution least-squares estimation modulation data demonstrate super-resolution mapping (SDOM)...
1300 nm three-photon calcium imaging has emerged as a useful technique to allow in deep brain regions. Application large-scale neural activity entails careful balance between recording fidelity and perturbation the sample. We calculated experimentally verified excitation pulse energy achieve minimum photon count required for detection of transients GCaMP6s-expressing neurons 920 two-photon 1320 excitation. By considering combined effects in-focus signal attenuation out-of-focus background...
Axial excitation confinement beyond the diffraction limit is crucial to development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral using a donut-beam depletion, but its axial resolution still over 500 nm. Total internal reflection fluorescence microscopy widely used for single-molecule localization, ability detect molecules limited within evanescent field ~100 nm from cell attachment surface. We find here that thickness point...
Quantum dots (QDs) are well known as bright and photostable inorganic fluorescent probes for microscopy imaging, with many attractive features superior to those found in organic dyes. However, their broadband excitation spectrum emission blinking property have limited the applicability of QDs modern super-resolution techniques. In this work, we systematically investigate practical approaches overcoming these drawbacks provide examples use across commercially available systems now accessible...
The orientation of fluorophores can reveal crucial information about the structure and dynamics their associated subcellular organelles. Despite significant progress in super-resolution, fluorescence polarization microscopy remains limited to unique samples with relatively strong modulation not applicable weak signals due excessive background noise. Here we apply optical lock-in detection amplify super-resolution. This novel technique, termed super-resolution dipole mapping (OLID-SDOM),...
Three-photon microscopy (3PM) was shown to allow deeper imaging than two-photon (2PM) in scattering biological tissues, such as the mouse brain, since longer excitation wavelength reduces tissue and higher-order non-linear suppresses out-of-focus background fluorescence. Imaging depth resolution can further be improved by aberration correction using adaptive optics (AO) techniques where a spatial light modulator (SLM) is used correct wavefront aberrations. Here, we present analyze 3PM AO...
Stimulated emission depletion (STED) and structured illumination (SIM) are two commonly used techniques for super-resolution imaging. However, the performance of these has never been quantitatively compared side-by-side. Taking advantage non-photobleaching characteristic NV centres in fluorescent nanodiamond (FND), we performed a comparative study resolution methods with 35 nm FNDs at single particle level, as well FND grown bulk diamond material. Results show that STED provides more...
Bovine follicular oocytes were collected from ovarian antral follicles (2 to 7 mm in diameter) slaughtered cattle. They matured vitro (IVM) for 23 24 h and then activated. In Experiment 1, 4 concentrations of ethanol compared. The activation rates 4, 12, 36 27%, respectively, following exposure min 0, 5, 10% ethanol. 2, 7% was tested with times 7.5 10 min, 6, 32, 27 33% the activated, respectively. 3 synergistic effect electric pulse compared within treatments: A) alone, B) C) first...
By manipulating the spectral dispersion of detected photons, spectroscopic single-molecule localization microscopy (sSMLM) permits concurrent high-throughput single-molecular analysis and imaging. Despite its promising potential, using discrete optical components managing delicate balance between spatial compromise performance, including non-uniform dispersion, high transmission loss grating, alignment demands, reduced precision. We designed a dual-wedge prism (DWP)-based monolithic imaging...
Abstract Sub-diffraction limited localization of fluorescent emitters is a key goal microscopy imaging. Here, we report that single upconversion nanoparticles, containing multiple emission centres with random orientations, can generate series unique, bright and position-sensitive patterns in the spatial domain when placed on top mirror. Supported by our numerical simulation, attribute this effect to sum each emitter’s interference its own mirror image. As result, configuration generates...
The broad applicability of super-resolution microscopy has been widely demonstrated in various areas and disciplines. optimization improvement algorithms used are great importance for achieving optimal quality imaging. In this review, we comprehensively discuss the computational methods different types microscopy, including deconvolution polarization-based structured illumination image scanning optical fluctuation imaging single-molecule localization Bayesian stimulated emission depletion...
Rabbit embryonic stem-like cells, characterized by embryoid body formation and differentiation into cell types representative of all three germ layers, were studied for their ability to promote early development after nuclear transfer. After culture the reconstructed embryos, 23% (n = 35) developed successfully morulae or blastocysts, compared with 34% 62) cloned embryos derived from transfer blastomeres. The cells appeared normal, an average 26% inner mass similar that control...
Data are presented showing that lithographic vertical‐cavity surface‐emitting lasers (VCSELs) produce minimal junction temperature rise compared to oxide VCSELs. Eliminating the thermal block caused by internal oxides combined with improved mirror materials reduces temperature. The elimination of oxide, lower and reduced strain promise increased reliability in new Power conversion efficiencies excess ∼50% reported, even for very small
Abstract Upconverting luminescent nanoparticles are photostable, nonblinking, and low chemically toxic fluorophores that emerging as promising fluorescent probes at the single molecule level. High luminescence intensity upconversion (UCNPs) have previously been achieved by doping with high amounts of rare-earth ions using excitation power (>2.5 MW/cm 2 ). However, such particles inadequate for in vitro live-cell imaging single-particle tracking, can cause photodamage. Here, we compared...
Light-field particle imaging velocimetry (LF-PIV) is widely used in large-scale flow field measurement scenarios due to its instant 3D capability. However, conventional LF-PIV systems suffer low axial resolution and thereby have limited application high-resolution volumetric velocity measurements. Here, we report the use of synthetic color-and-depth-encoded (sCade) illumination improve LF-PIV. The sCade illuminated region with a color-and-depth encoded beam synthesized by structured beams...
The recent advances in chromosome configuration capture (3C)‐based series molecular methods and optical super‐resolution (SR) techniques offer powerful tools to investigate three dimensional (3D) genomic structure prokaryotic eukaryotic cell nucleus. In this review, we focus on the progress during last decade exciting field. Here at first introduce briefly genome organization chromosome, domain sub‐domain level, respectively; then provide a short introduction various microscopy which can be...