A5038613907- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Molecular Sensors and Ion Detection
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Nanoplatforms for cancer theranostics
- Optical Coherence Tomography Applications
- Analytical Chemistry and Sensors
- Photodynamic Therapy Research Studies
- Image Processing Techniques and Applications
- Near-Field Optical Microscopy
- Lipid Membrane Structure and Behavior
- Photochemistry and Electron Transfer Studies
- Carbon and Quantum Dots Applications
- Nonlinear Optical Materials Studies
- Photosynthetic Processes and Mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- Electron Spin Resonance Studies
- Photoreceptor and optogenetics research
- Sphingolipid Metabolism and Signaling
- 2D Materials and Applications
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
Shenzhen University
2016-2025
Ningxia Medical University
2024-2025
Harbin Engineering University
2016-2024
North China University of Science and Technology
2013-2024
Yanshan University
2012-2023
University of Hong Kong
2022
Shanghai University of Medicine and Health Sciences
2021
Jiading District Central Hospital
2021
Beijing University of Chinese Medicine
2020
Korea University
2012-2018
Abstract Nonlayered materials are constructed with chemical covalent bonds in all three dimensions, distinct from layered materials, which contain evident structural differences the horizontal and vertical directions. As a consequence, liquid‐phase exfoliation (LPE), widely explored technique to obtain 2D nanoarchitectures, has not yet been fully characterized for realization of nonlayered nanostructures. Herein, by virtue typical chain‐like structure crystalline bulk Te strong TeTe...
A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer mitochondria-targeting unit, was synthesized. The showed direct linear relationship between the fluorescence intensity ratio BODIPY to or lifetime media viscosity, which allowed us determine average mitochondrial in living HeLa cells as ca. 62 cP (cp). Upon treatment an ionophore, monensin, nystatin, observed increase 110 cP.
Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules, diffusion reactive metabolites in live cells. Fluorescent molecular rotors are recently developed reagents used to determine solutions or biological fluid. Due complexity cells, it is important carry out determinations multimode for high reliability accuracy. The first rotor (RY3) capable dual mode fluorescence imaging (ratiometry lifetime imaging) intracellular...
A series of heptamethine cyanine (1–3) derivatives bearing a carbamate ethyl disulfide group and gemcitabine, an anticancer drug, have been newly synthesized. Their bonds are readily cleaved by various thiols including glutathione, to result in subsequent decomposition the into amine followed release active which can be monitored fluorescence changes. In biological experiment, prodrug 1 is preferentially up-taken folate-positive KB cells over folate-negative A549 via receptor-mediated...
Bis-thiocarbono-hydrazones are found to be a class of sensitive, selective, ratiometric, and colorimetric chemosensors for anions such as fluoride (F(-)) or acetate (Ac(-)). The sensitivities, the binding constants sensors with anions, were strongly dependent on substituents appended pi-conjugation framework, delocalization bridge CH==N, aromatic moiety, hetero atom in C==X group (X=O, S) sensors. Single-crystal structures (1)H NMR titration analysis shows that --CH==N-- moiety is...
A specific ratiometric nanoprobe for hypochlorite was constructed as a paradigm of FRET spectral unmixing. The separation pairs' emissions reaches 175 nm, which ensures that the probing is more accurate. This new shows high selectivity and potential in biological systems.
In the thick of it: A carbazole-based cyanine derivative has been used as a ratiometric fluorescent sensor to detect mitochondrial viscosities over range 1–950 cP within live cells and living tissues by using two-photon microscopy. Its success is based on fast- low-barrier rotation around its methine bond (see figure) huge increase fluorescence quantum yield (QY) from loss mobility in viscous media.
Abstract Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve regulate functionality. Conventional fluorescence microscopy can only provide ~300 nm resolution, which insufficient visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) study dynamic cristae live cells with superresolution technique. The low saturation intensity...
We herein report a fluorescent bimodal probe (1) capable of determining ER viscosity and polarity changes using FLIM fluorescence ratiometry, respectively; during stress caused by tunicamycin, the was increased from ca. 129.5 to 182.0 cP (dielectric constant, ε) enhanced 18.5 21.1.
Abstract Flexible organic materials that exhibit dynamic ultralong room temperature phosphorescence (DURTP) via photoactivation have attracted increasing research interest for their fascinating functions of reversibly writing-reading-erasing graphic information in the form a long afterglow. However, due to existence nonnegligible activation threshold initial exposure dose, display mode these has thus far been limited binary patterns. By resorting halogen element doping carbon dots (CDs)...
Abstract Super-resolution optical imaging is crucial to the study of cellular processes. Current super-resolution fluorescence microscopy restricted by need special fluorophores or sophisticated systems, long acquisition and computational times. In this work, we present a deep-learning-based technique confocal microscopy. We devise two-channel attention network (TCAN), which takes advantage both spatial representations frequency contents learn more precise mapping from low-resolution images...
A dual functional fluorescent molecular rotor was developed to trigger intracellular ER autophagy and quantify the local viscosity variations by FLIM imaging.
Various strategies for TSQ-induced fluorophore stabilization and their application in sm-FRET as well super-resolution imaging microscopy are thoroughly reviewed.
A versatile twisted-intramolecular-charge-transfer (TICT)-based near-infrared (NIR) fluorescent probe (L) has been judiciously designed and synthesized that could be utilized for potential cancer diagnosis to track lymph node(s) in mice through distinct emission signals. Essentially, the rendered capability preferentially recognize cells over noncancer by polarity-guided lipid droplet specific differential bioimaging (in green channel) studies. The also exhibited selective turn-on...
Stimulated emission depletion (STED) microscopy is a powerful super-resolution technique that has achieved significant results in breaking the resolution limit and relevant applications.