A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer mitochondria-targeting unit, was synthesized. The showed direct linear relationship between the fluorescence intensity ratio BODIPY to or lifetime media viscosity, which allowed us determine average mitochondrial in living HeLa cells as ca. 62 cP (cp). Upon treatment an ionophore, monensin, nystatin, observed increase 110 cP.

We report here a mitochondria-targetable pH-sensitive probe that allows for quantitative measurement of mitochondrial pH changes, as well the real-time monitoring pH-related physiological effects in live cells. This system consists piperazine-linked naphthalimide fluorescence off–on signaling unit, cationic triphenylphosphonium group targeting, and reactive benzyl chloride subunit fixation. It operates environment within whole cells displays desirable response to acidification. Moreover,...

We present here, the design, synthesis, spectroscopic characterization, and in vitro biological assessment of a gemcitabine-coumarin-biotin conjugate (5). Probe 5 is multifunctional molecule composed thiol-specific cleavable disulfide bond, coumarin moiety as fluorescent reporter, gemcitabine (GMC) model active drug, biotin cancer-targeting unit. Upon addition free thiols that are relatively abundant tumor cells, bond cleavage occurs well drug GMC release concomitantly fluorescence intensity...

Hightech-pH-Indikator: Ein pH-"Chamäleon" aus Rhodamin- (rot, siehe Schema) und Fluorescein-Einheiten (grün) emittiert bei Wellenlängen von 580 nm 512 nm, wo die Intensitäten gegenläufig auf pH-Änderungen ansprechen. Konfokalmikroskopische Aufnahmen HeLa-Zellen mit diesem Indikator zeigen rote grüne Flecken; das Verhältnis dieser Signale kann kalibriert daraus der pH-Wert Organelle ermittelt werden. As a service to our authors and readers, this journal provides supporting information...

A series of heptamethine cyanine (1–3) derivatives bearing a carbamate ethyl disulfide group and gemcitabine, an anticancer drug, have been newly synthesized. Their bonds are readily cleaved by various thiols including glutathione, to result in subsequent decomposition the into amine followed release active which can be monitored fluorescence changes. In biological experiment, prodrug 1 is preferentially up-taken folate-positive KB cells over folate-negative A549 via receptor-mediated...

Two novel Cu²⁺sensors, bearing naphthalimide and a DPA moiety, were synthesized to study copper accumulation in organelles serve as valuable tool elucidate the subcellular location of ions.

We present the design, synthesis, optical properties and in vitro biological assessments of theranostic prodrug 6 which a near IR fluorophore is conjugated with cancer cell-directing biotin unit; further it linked anti-cancer drug gemcitabine via self-immolative spacer, disulfide bond. The able to monitor delivery cellular imaging.

Thioredoxin (Trx) is a redox-active protein that plays key role in mitigating the effects of oxidative stress. The secretion Trx on plasma membrane has been suggested as distinctive feature inflammation. However, selective monitoring membrane-associated activity proved challenging because ubiquity action cells. Here, we report Trx-specific probe allows visualization associated with membranes via fluorescence microscopy. ability this to act possible screening tool for agents modulate was...

We investigated the effect of two brush parameters, thickness and dispersity in molecular weight distribution, on adhesion bacteria to pH-responsive poly(acrylic acid) (PAA) brushes synthesized using surface-initiated atom transfer radical polymerization. The attachment detachment Staphylococcus epidermidis PAA at pH 4 9, respectively, were examined with confocal microscopy. An optimal range thickness, 13-18 nm, was identified for minimizing bacterial 4, did not depend dispersity. Increasing...

Increased shear thinning arising due to strong attractive interactions between colloidal particles is thought obscure thickening. Here, we demonstrate how moderate attractions, induced by adding a nonadsorbing polymer, can instead enhance We measure the rheology of suspensions at constant particle volume fraction ϕ=0.40 with dilute weakly semidilute concentrations three polyacrylamide depletants different molecular weights. Suspensions containing large polymer exhibit increased thickening...

Abstract Background Many studies have been performed to identify various genomic loci and genes associated with the meat quality in pigs. However, full genetic architecture of trait still remains unclear part because lack accurate identification related structural variations (SVs) which resulted from shortage target breeds, limitations sequencing data, incompleteness genome assemblies. The recent generation a new pig breed superior quality, called Nanchukmacdon, its chromosome-level assembly...

Pig as a food source serves daily dietary demand to wide population around the world. Preference of meat depends on various factors with muscle play central role. In this regards, selective breeding abled us develop "Nanchukmacdon" pig breeds an enhanced variety and high fertility rate. To identify genomic regions under selection we performed whole-genome resequencing, transcriptome, bisulfite sequencing from Nanchukmacdon muscles samples used published data for three other such Landrace,...

Abstract Background DNA methylation is an important epigenetic modification that known to regulate gene expression. Whole-genome bisulfite sequencing (WGBS) a powerful method for studying cytosine in whole genome. However, it difficult obtain profiles using the WGBS raw reads and necessary be proficient all types of bioinformatic tools study methylation. In addition, recent end-to-end pipelines analyses are not sufficient addressing those difficulties. Results Here we present msPIPE,...

Abstract Advancements in next-generation sequencing (NGS) technologies have led to a substantial increase the availability of population genetic variant data, thus prompting development various analysis tools enhance our understanding structure and evolution. The that are currently used analyze data generally require different environments, parameters, formats input which can act as barrier preventing wide-spread usage such by general researchers who may not be familiar with bioinformatics....

Abstract As plentiful high-quality genome assemblies have been accumulated, reference-guided assembly can be a good approach to reconstruct assembly. Here, we present chromosome-level of the Korean crossbred pig called Nanchukmacdon (the NCMD assembly) using with short and long reads. The contains 20 scaffolds total size 2.38 Gbp (N50: 138.77 Mbp). Its BUSCO score is 93.1%, which comparable reference assembly, 20,588 protein-coding genes, 8,651 non-coding 996.14 Mbp repetitive elements are...

The phase behavior of colloid–polymer mixtures containing unary or binary polymers as depletants could be superimposed onto a single diagram using an effective polymer concentration.

Baculovirus expression systems have been widely used to produce recombinant mammalian proteins owing the lack of viral replication in vertebrates. Although several lines evidence demonstrated impacts baculovirus infection hosts, genome-wide effects not fully elucidated. Here, we provide comparative transcriptome profiles and host-immune response genes baculovirus-infected insect cells. Specifically, decipher cells, conducted total RNA-seq on human 293TT cells Sf9 infected with baculovirus....

Metagenomic assembly using high-throughput sequencing data is a powerful method to construct microbial genomes in environmental samples without cultivation. However, metagenomic assembly, especially when only short reads are available, complex and challenging task because mixed of multiple microorganisms constitute the metagenome. Although long read technologies have been developed begun be used for many studies performed based on generation requires higher cost than reads.In this study, we...

We developed a model depletion system with colloidal particles that were refractive index- and density-matched to 80 (w/w)% glycerol in water, characterized the effect of interparticle interactions on structure dynamics non-equilibrium phases. 2,2,2-trifluoroethyl methacrylate-co-tert-butyl methacrylate copolymer synthesized following Kodger et al. (Sci. Rep. 5, 14635 (2015)). Particles dispersed glycerol/water solutions generate suspensions good control over electrostatic moderately high...

We report a colloid-polymer model system with tunable bridging interactions for microscopic studies of structure and dynamics using confocal imaging. The between trifluoroethyl methacrylate-co-tert-butyl methacrylate copolymer particles poly(acrylic acid) (PAA) polymers were controllable via polymer concentration pH. strength adsorption PAA on the particles, driven by pH-dependent brush stabilizers particle surfaces, was tuned solution Particle-polymer suspensions formulated at low pH, where...

본 연구의 목적은 알럼 슬러지를 사용하여 입상흡착제를 제조하기 위한 압출 공정을 최적화하는 것이다. 함수율과 바인더인 메틸 셀룰로스의 함량이 가능성과 입상흡착제의 물리적 특성에 미치는 영향을 연구하였다. 특성은 질소 흡착실험과 압축 강도 측정을 통해서 분석하였다. 증류수와 슬러지 가공분말의 비가 63/100인 경우가 외형이 원통형으로 잘 성형되었고, 압축강도도 가장 높게 나타났다. 함량을 증가시키면 압축강도가 개선되었으나 비표면적이 감소하였다. 성형된 소성과정을 거치면 표면적이 크게 증가하여 황화수소의 파과 시간이 획기적으로 증가하였으며, 황화수소 용량은 1,700 mg/g 이상을 얻을 수 있었다. The objective of this study is optimization extrusion process for preparation pellet-type adsorbents employed with alum sludge. Effects water content and...

본 연구에서는 정수장 슬러지를 사용하여 제조한 정수슬러지 가공 분말의 압출공정을 통해서 입상흡착제를 제조하였다. 바인더 첨가와 소성과정이 입상형 흡착제의 물리 화학적 특성에 미치는 영향을 질소흡착, 압축강도, 주사전자현미경, 엑스선회절, 피리딘흡착 적외선분광법 등으로 분석하였다. 바인더의 함량을 5 wt%까지 증가시키면 압축강도가 3배 이상으로 개선되었으나 트리메틸아민을 흡착할 수 있는 표면적이 30% 정도 감소하여 입상흡착제의 트리메틸아민 파과시간이 단축되었다. 성형된 소성과정을 표면에 브뢴스테드산점과 루이스산점으로 구성된 산점이 발현되어, 염기성 기체인 트리메틸아민의 파과 시간이 증가하였다. In this study, the pellet-type adsorbents were prepared by extrusion using water treatment sludge. Effects of binder and calcination on physical chemical...

This study aims to assess the impact of cultural heritage night tours—a policy initiative leveraging assets—on regional image and visitation intent among travel industry professionals. By reviewing prior research, this identifies measurement variables for attributes tours (attractiveness, uniqueness, educational value, convenience), image, intent. The analysis shows that while all positively affect only value convenience influence can be attributed characteristics professionals surveyed, who...