A5066379209- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- RNA regulation and disease
- Chromosomal and Genetic Variations
Harvard University
2017-2020
École Polytechnique
2017-2020
Centre National de la Recherche Scientifique
2017-2020
Inserm
2017-2020
Université Paris-Saclay
2017
Abstract To extend the frontier of genome editing and enable repetitive elements mammalian genomes, we made use a set dead-Cas9 base editor (dBE) variants that allow at tens thousands loci per cell by overcoming death associated with DNA double-strand breaks single-strand breaks. We used gRNAs targeting elements—ranging in target copy number from about 32 to 161 000 cell. dBEs enabled survival after large-scale editing, allowing targeted mutations up ∼13 200 ∼12 293T human induced...
Multiplex genome editing is the simultaneous introduction of multiple distinct modifications to a given genome. Though in its infancy, maturation this field will facilitate powerful new biomedical research approaches and enable host far-reaching biological engineering applications, including therapeutic modalities industrial as well "genome writing" de-extinction efforts. In Perspective, we focus on multiplex large eukaryotic genomes. We describe current state multiplexed editing, limits our...
Abstract To extend the frontier of genome editing and enable radical redesign mammalian genomes, we developed a set dead-Cas9 base editor (dBE) variants that allow at tens thousands loci per cell by overcoming death associated with DNA double-strand breaks (DSBs) single-strand (SSBs). We used gRNAs targeting repetitive elements – ranging in target copy number from about 31 to 124,000 cell. dBEs enabled survival after large-scale editing, allowing targeted mutations up ~13,200 ~2610 293T...
Abstract We have exploited the repetitive nature of transposable elements human genome to generate synthetic circuits. Transposable such as LINE-1 and Alu successfully replicated in mammalian genomes throughout evolution reach a copy number ranging from thousands more than million. Targeting these with programmable DNA nucleases CRISPR-Cas9 rapidly induce extremely high levels cell death. use this genotoxic feature build biocontainment circuits: CRISPR defense system (CRISPR-DS) capable...